Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina
This study was conducted to detect Borrelia burgdorferi sensu lato infection in ixodid ticks from the Patagonia region in the south of Argentina. Therefore, ticks were collected on rodents in the provinces of Chubut, Río Negro and Santa Cruz. These ticks were identified as nymphs of Ixodes cf. neuqu...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/918 http://www.sciencedirect.com/science/article/pii/S0001706X16302200?via%3Dihub https://doi.org/10.1016/j.actatropica.2016.06.030 |
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| author | Saracho Bottero, María Noelia Lareschi, Marcela Venzal, José Manuel Carvalho, Luis A. Nava, Santiago Sebastian, Patrick Stephan Mackenstedt, Ute |
| author_browse | Carvalho, Luis A. Lareschi, Marcela Mackenstedt, Ute Nava, Santiago Saracho Bottero, María Noelia Sebastian, Patrick Stephan Venzal, José Manuel |
| author_facet | Saracho Bottero, María Noelia Lareschi, Marcela Venzal, José Manuel Carvalho, Luis A. Nava, Santiago Sebastian, Patrick Stephan Mackenstedt, Ute |
| author_sort | Saracho Bottero, María Noelia |
| collection | INTA Digital |
| description | This study was conducted to detect Borrelia burgdorferi sensu lato infection in ixodid ticks from the Patagonia region in the south of Argentina. Therefore, ticks were collected on rodents in the provinces of Chubut, Río Negro and Santa Cruz. These ticks were identified as nymphs of Ixodes cf. neuquenensis and Ixodes sigelos. The B. burgdorferi s.l. infection was tested by a battery of PCR methods targeting the gene flagellin (fla) and the rrfA-rrlB intergenic spacer region (IGS). Three pools of I. sigelos nymphs from Chubut and Santa Cruz provinces as well as one pool of I. cf. neuquenensis nymphs from Río Negro province were tested positive in the fla-PCR. The samples of I. sigelos were also positive for the IGS-PCR. Phylogenetically, the haplotypes found in the positive ticks belong to the B. burgdorferi s.l. complex, and they were closely related to Borrelia chilensis, a genospecies isolated from Ixodes stilesi in Chile. The pathogenic relevance of the Borrelia genospecies detected in both I. neuquenensis and I. sigelos is unknown |
| format | info:eu-repo/semantics/article |
| id | INTA918 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9182020-04-13T14:32:35Z Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina Saracho Bottero, María Noelia Lareschi, Marcela Venzal, José Manuel Carvalho, Luis A. Nava, Santiago Sebastian, Patrick Stephan Mackenstedt, Ute Ixodes Metastigmata Borrelia burgdorferi Garrapatas Región Patagónica This study was conducted to detect Borrelia burgdorferi sensu lato infection in ixodid ticks from the Patagonia region in the south of Argentina. Therefore, ticks were collected on rodents in the provinces of Chubut, Río Negro and Santa Cruz. These ticks were identified as nymphs of Ixodes cf. neuquenensis and Ixodes sigelos. The B. burgdorferi s.l. infection was tested by a battery of PCR methods targeting the gene flagellin (fla) and the rrfA-rrlB intergenic spacer region (IGS). Three pools of I. sigelos nymphs from Chubut and Santa Cruz provinces as well as one pool of I. cf. neuquenensis nymphs from Río Negro province were tested positive in the fla-PCR. The samples of I. sigelos were also positive for the IGS-PCR. Phylogenetically, the haplotypes found in the positive ticks belong to the B. burgdorferi s.l. complex, and they were closely related to Borrelia chilensis, a genospecies isolated from Ixodes stilesi in Chile. The pathogenic relevance of the Borrelia genospecies detected in both I. neuquenensis and I. sigelos is unknown EEA Rafaela Fil: Saracho Bottero, Maria Noelia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lareschi, Marcela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico La Plata. Centro de Estudios Parasitológicos y de Vectores; Argentina Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Carvalho, Luis A. Universidad de la República. CENUR Litoral Norte-Salto. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sebastian, Patrick S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Grupo Gestión Ambiental y Suelos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Hohenheim. Parasitology Unit; Alemania Fil: Mackenstedt, Ute. University of Hohenheim. Parasitology Unit; Alemania 2017-08-08T12:10:53Z 2017-08-08T12:10:53Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/918 http://www.sciencedirect.com/science/article/pii/S0001706X16302200?via%3Dihub 0001-706X https://doi.org/10.1016/j.actatropica.2016.06.030 eng info:eu-repo/semantics/restrictedAccess application/pdf Acta tropica 162 : 218-221. (2016) |
| spellingShingle | Ixodes Metastigmata Borrelia burgdorferi Garrapatas Región Patagónica Saracho Bottero, María Noelia Lareschi, Marcela Venzal, José Manuel Carvalho, Luis A. Nava, Santiago Sebastian, Patrick Stephan Mackenstedt, Ute Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title | Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title_full | Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title_fullStr | Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title_full_unstemmed | Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title_short | Borrelia burgdorferi sensu lato in Ixodes cf. neuquenensis and Ixodes sigelos ticks from the Patagonian region Argentina |
| title_sort | borrelia burgdorferi sensu lato in ixodes cf neuquenensis and ixodes sigelos ticks from the patagonian region argentina |
| topic | Ixodes Metastigmata Borrelia burgdorferi Garrapatas Región Patagónica |
| url | http://hdl.handle.net/20.500.12123/918 http://www.sciencedirect.com/science/article/pii/S0001706X16302200?via%3Dihub https://doi.org/10.1016/j.actatropica.2016.06.030 |
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