The heart microbiome of insectivorous bats from Central and South Eastern Europe
Host associated microbiome not only may affect the individual health-status or provide insights into the species- or group specific bacterial communities but may act as early warning signs in the assessment of zoonotic reservoirs, offering clues to predict, prevent and control possible episodes of e...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Elsevier
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9160 https://www.sciencedirect.com/science/article/pii/S0147957120301946 https://doi.org/10.1016/j.cimid.2020.101605 |
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| author | Corduneanu, Alexandra Mihalca, Andrei Daniel Sandor, Attila D. Hornok, Sándor Malmberg, Maja Pin Viso, Natalia Daniela Bongcam-Rudloff, Erik |
| author_browse | Bongcam-Rudloff, Erik Corduneanu, Alexandra Hornok, Sándor Malmberg, Maja Mihalca, Andrei Daniel Pin Viso, Natalia Daniela Sandor, Attila D. |
| author_facet | Corduneanu, Alexandra Mihalca, Andrei Daniel Sandor, Attila D. Hornok, Sándor Malmberg, Maja Pin Viso, Natalia Daniela Bongcam-Rudloff, Erik |
| author_sort | Corduneanu, Alexandra |
| collection | INTA Digital |
| description | Host associated microbiome not only may affect the individual health-status or provide insights into the species- or group specific bacterial communities but may act as early warning signs in the assessment of zoonotic reservoirs, offering clues to predict, prevent and control possible episodes of emerging zoonoses. Bats may be carriers and reservoirs of multiple pathogens such as viruses, bacteria and parasites, showing in the same time robust immunity against many of them. The microbiota plays a fundamental role on the induction, training and function of the host immune system and the immune system has largely evolved in order to maintain the symbiotic relationship of the host with these diverse microbes. Thus, expanding our knowledge on bat-associated microbiome it can be usefully in understanding bats’ outstanding immune capacities. The aim of this study was to investigate the presence of different bacterial communities in heart tissue of insectivorous bats, Nyctalus noctula, Pipistrellus pipistrellus and Rhinoplophus hipposideros, from Central and Eastern Europe using high-throughput sequencing of variable regions of the 16S rRNA. In addition, species-specific PCRs were used to validate the presence of the vector-borne pathogens Bartonella spp. and Rickettsia spp. In this study we identified a wide variety of bacterial groups, with the most abundant phyla being Proteobacteria and Firmicutes. The results showed that at individual level, the year or location had no effect on the diversity and composition of the microbiome, however host species determined both structure and abundance of the bacterial community. We report the presence of vector-borne bacteria Bartonella spp. in samples of N. noctula and indications of Rickettsia spp. in R. hipposideros. Our results provide a first insight into the bacterial community found in heart tissue of bats from Central and South Eastern Europe. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9160 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA91602021-04-22T16:56:31Z The heart microbiome of insectivorous bats from Central and South Eastern Europe Corduneanu, Alexandra Mihalca, Andrei Daniel Sandor, Attila D. Hornok, Sándor Malmberg, Maja Pin Viso, Natalia Daniela Bongcam-Rudloff, Erik Bacteria Chiroptera Zoonosis Parásitos Microbiología Europa Bartonella Rickettsia Zoonoses Parasites Microbiology Europe Nyctalus noctula Pipistrellus pipistrellus Rhinoplophus hipposideros Host associated microbiome not only may affect the individual health-status or provide insights into the species- or group specific bacterial communities but may act as early warning signs in the assessment of zoonotic reservoirs, offering clues to predict, prevent and control possible episodes of emerging zoonoses. Bats may be carriers and reservoirs of multiple pathogens such as viruses, bacteria and parasites, showing in the same time robust immunity against many of them. The microbiota plays a fundamental role on the induction, training and function of the host immune system and the immune system has largely evolved in order to maintain the symbiotic relationship of the host with these diverse microbes. Thus, expanding our knowledge on bat-associated microbiome it can be usefully in understanding bats’ outstanding immune capacities. The aim of this study was to investigate the presence of different bacterial communities in heart tissue of insectivorous bats, Nyctalus noctula, Pipistrellus pipistrellus and Rhinoplophus hipposideros, from Central and Eastern Europe using high-throughput sequencing of variable regions of the 16S rRNA. In addition, species-specific PCRs were used to validate the presence of the vector-borne pathogens Bartonella spp. and Rickettsia spp. In this study we identified a wide variety of bacterial groups, with the most abundant phyla being Proteobacteria and Firmicutes. The results showed that at individual level, the year or location had no effect on the diversity and composition of the microbiome, however host species determined both structure and abundance of the bacterial community. We report the presence of vector-borne bacteria Bartonella spp. in samples of N. noctula and indications of Rickettsia spp. in R. hipposideros. Our results provide a first insight into the bacterial community found in heart tissue of bats from Central and South Eastern Europe. Instituto de Biotecnología Fil: Corduneanu, Alexandra. University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca. Department of Parasitology and Parasitic Diseases; Rumania Fil: Mihalca, Andrei Daniel. University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca. Department of Parasitology and Parasitic Diseases; Rumania Fil: Sandor, Attila D. University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca. Department of Parasitology and Parasitic Diseases; Rumania Fil: Sandor, Attila D. University of Veterinary Medicine. Department of Parasitology and Zoology; Hungría Fil: Hornok, Sándor. University of Veterinary Medicine. Department of Parasitology and Zoology; Hungría Fil: Malmberg, Maja. Swedish University of Agricultural Sciences. Department of Biomedical Sciences and Veterinary Public Health. Section of Virology; Suecia Fil: Malmberg, Maja. Swedish University of Agricultural Sciences. Department of Animal Breeding and Genetics. SLU Global Bioinformatics Centre; Suecia Fil: Pin Viso, Natalia Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bongcam-Rudloff, Erik. Swedish University of Agricultural Sciences. Department of Animal Breeding and Genetics. SLU Global Bioinformatics Centre; Suecia 2021-04-22T16:47:54Z 2021-04-22T16:47:54Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9160 https://www.sciencedirect.com/science/article/pii/S0147957120301946 1878-1667 https://doi.org/10.1016/j.cimid.2020.101605 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 75 : 101605 (Abril 2021) |
| spellingShingle | Bacteria Chiroptera Zoonosis Parásitos Microbiología Europa Bartonella Rickettsia Zoonoses Parasites Microbiology Europe Nyctalus noctula Pipistrellus pipistrellus Rhinoplophus hipposideros Corduneanu, Alexandra Mihalca, Andrei Daniel Sandor, Attila D. Hornok, Sándor Malmberg, Maja Pin Viso, Natalia Daniela Bongcam-Rudloff, Erik The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title | The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title_full | The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title_fullStr | The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title_full_unstemmed | The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title_short | The heart microbiome of insectivorous bats from Central and South Eastern Europe |
| title_sort | heart microbiome of insectivorous bats from central and south eastern europe |
| topic | Bacteria Chiroptera Zoonosis Parásitos Microbiología Europa Bartonella Rickettsia Zoonoses Parasites Microbiology Europe Nyctalus noctula Pipistrellus pipistrellus Rhinoplophus hipposideros |
| url | http://hdl.handle.net/20.500.12123/9160 https://www.sciencedirect.com/science/article/pii/S0147957120301946 https://doi.org/10.1016/j.cimid.2020.101605 |
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