Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G prot...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9147 https://www.sciencedirect.com/science/article/pii/S0022175920302350 https://doi.org/10.1016/j.jim.2020.112941 |
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| author | Alonso, Maria Natalia Griffa, Natanael Moyano, Roberto Damián Mon, Maria Laura Colombatti Olivieri, Maria Alejandra Barandiaran, Soledad Martinez Vivot, Marcela Fiorini, Gonzalo Canal, Ana María Santangelo, María De La Paz Singh, Mahavir G. Romano, Maria Isabel |
| author_browse | Alonso, Maria Natalia Barandiaran, Soledad Canal, Ana María Colombatti Olivieri, Maria Alejandra Fiorini, Gonzalo Griffa, Natanael Martinez Vivot, Marcela Mon, Maria Laura Moyano, Roberto Damián Romano, Maria Isabel Santangelo, María De La Paz Singh, Mahavir G. |
| author_facet | Alonso, Maria Natalia Griffa, Natanael Moyano, Roberto Damián Mon, Maria Laura Colombatti Olivieri, Maria Alejandra Barandiaran, Soledad Martinez Vivot, Marcela Fiorini, Gonzalo Canal, Ana María Santangelo, María De La Paz Singh, Mahavir G. Romano, Maria Isabel |
| author_sort | Alonso, Maria Natalia |
| collection | INTA Digital |
| description | Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard).
No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB. |
| format | Artículo |
| id | INTA9147 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA91472021-04-21T16:08:13Z Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis Alonso, Maria Natalia Griffa, Natanael Moyano, Roberto Damián Mon, Maria Laura Colombatti Olivieri, Maria Alejandra Barandiaran, Soledad Martinez Vivot, Marcela Fiorini, Gonzalo Canal, Ana María Santangelo, María De La Paz Singh, Mahavir G. Romano, Maria Isabel Immunological Techniques Mycobacterium bovis Infections Chromatography Tests Antibodies Técnicas Inmunológicas Mycobacterium bovis Tuberculosis Infeccion por Mycobacterium bovis Bovinae Cromatografía Anticuerpos Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard). No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB. Instituto de Biotecnología Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Griffa Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Fiorini, Gonzalo. General Pinto; Argentina Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Singh, Mahavir G. Lionnex Diagnostics & Therapeutics GmbH; Alemania Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-04-21T15:54:09Z 2021-04-21T15:54:09Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9147 https://www.sciencedirect.com/science/article/pii/S0022175920302350 1872-7905 https://doi.org/10.1016/j.jim.2020.112941 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Immunological Methods 491 : 112941 (Abril 2021) |
| spellingShingle | Immunological Techniques Mycobacterium bovis Infections Chromatography Tests Antibodies Técnicas Inmunológicas Mycobacterium bovis Tuberculosis Infeccion por Mycobacterium bovis Bovinae Cromatografía Anticuerpos Alonso, Maria Natalia Griffa, Natanael Moyano, Roberto Damián Mon, Maria Laura Colombatti Olivieri, Maria Alejandra Barandiaran, Soledad Martinez Vivot, Marcela Fiorini, Gonzalo Canal, Ana María Santangelo, María De La Paz Singh, Mahavir G. Romano, Maria Isabel Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title | Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title_full | Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title_fullStr | Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title_full_unstemmed | Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title_short | Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| title_sort | development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis |
| topic | Immunological Techniques Mycobacterium bovis Infections Chromatography Tests Antibodies Técnicas Inmunológicas Mycobacterium bovis Tuberculosis Infeccion por Mycobacterium bovis Bovinae Cromatografía Anticuerpos |
| url | http://hdl.handle.net/20.500.12123/9147 https://www.sciencedirect.com/science/article/pii/S0022175920302350 https://doi.org/10.1016/j.jim.2020.112941 |
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