Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium
Despite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatili...
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer Nature
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9141 https://link.springer.com/article/10.1007/s00284-021-02467-z https://doi.org/10.1007/s00284-021-02467-z |
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| author | Hernandez Guijarro, Keren De Geronimo, Eduardo Erijman, Leonardo |
| author_browse | De Geronimo, Eduardo Erijman, Leonardo Hernandez Guijarro, Keren |
| author_facet | Hernandez Guijarro, Keren De Geronimo, Eduardo Erijman, Leonardo |
| author_sort | Hernandez Guijarro, Keren |
| collection | INTA Digital |
| description | Despite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was designed to target a specific region of the glycine oxidase gene (thiO), involved in the oxidation of glyphosate to AMPA, from known sequences of Bradyrhizobium species. The abundance of the thiO gene was determined in response to herbicide application in soils with different GP exposure history both under field and microcosm conditions. The gene coding for RNA polymerase subunitB (rpoB) was used as a reference for the abundance of total Bradyrhizobia. The assay using the designed primers was linear over a very large concentration range of the target and showed high efficiency and specificity. In a field experiment, there was a differential response related to the history of glyphosate use and the native Bradyrhizobium genotypes. In a soil without previous exposure to herbicides, thiO gene increased over time after glyphosate application with most genotypes belonging to the B. jicamae and B. elkanni supergroups. Conversely, in an agricultural soil with more than 10 years of continuous glyphosate application, the abundance of thiO gene decreased and most genotypes belonged to B. japonicum supergroup. In a microcosm assay, the amount of herbicide degraded after a single application was positively correlated to the number of thiO copies in different agricultural soils from the Pampean Region. Our results suggest that Bradyrhizobium species are differently involved in glyphosate degradation, denoting the existence of metabolically versatile microorganisms which can be explored for sustainable agriculture practices. The relationship between the abundance of thiO gene and the GP degraded in soil point to the use of thiO gene as a proxy for GP degradation in soil. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9141 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA91412021-04-21T11:42:28Z Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium Hernandez Guijarro, Keren De Geronimo, Eduardo Erijman, Leonardo Microorganismos Bradyrhizobium Degradación del Suelo Glifosato Microorganisms Soil Degradation Glyphosate Despite the intensive use of glyphosate (GP) and its ubiquitous presence in the environment, studies addressing the presence of microbial genes involved in glyphosate degradation in natural conditions are scarce. Based on the agronomical importance of Bradyrhizobium genus and its metabolic versatility, we tested the hypothesis that species or genotypes of Bradyrhizobium could be a proxy for GP degrader potential in soil. A quantitative PCR assay was designed to target a specific region of the glycine oxidase gene (thiO), involved in the oxidation of glyphosate to AMPA, from known sequences of Bradyrhizobium species. The abundance of the thiO gene was determined in response to herbicide application in soils with different GP exposure history both under field and microcosm conditions. The gene coding for RNA polymerase subunitB (rpoB) was used as a reference for the abundance of total Bradyrhizobia. The assay using the designed primers was linear over a very large concentration range of the target and showed high efficiency and specificity. In a field experiment, there was a differential response related to the history of glyphosate use and the native Bradyrhizobium genotypes. In a soil without previous exposure to herbicides, thiO gene increased over time after glyphosate application with most genotypes belonging to the B. jicamae and B. elkanni supergroups. Conversely, in an agricultural soil with more than 10 years of continuous glyphosate application, the abundance of thiO gene decreased and most genotypes belonged to B. japonicum supergroup. In a microcosm assay, the amount of herbicide degraded after a single application was positively correlated to the number of thiO copies in different agricultural soils from the Pampean Region. Our results suggest that Bradyrhizobium species are differently involved in glyphosate degradation, denoting the existence of metabolically versatile microorganisms which can be explored for sustainable agriculture practices. The relationship between the abundance of thiO gene and the GP degraded in soil point to the use of thiO gene as a proxy for GP degradation in soil. EEA Balcarce Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: De Gerónimo, Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: De Gerónimo, Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Erijman, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Erijman, Leonardo. Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2021-04-21T11:32:40Z 2021-04-21T11:32:40Z 2021-04-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9141 https://link.springer.com/article/10.1007/s00284-021-02467-z 0343-8651 1432-0991 https://doi.org/10.1007/s00284-021-02467-z eng info:eu-repograntAgreement/INTA/2019-PD-E2-I039-002/2019-PD-E2-I039-002/AR./REMEDIACIÓN DE SUELOS Y AGUAS Y RESTAURACIÓN ECOLÓGICA DE SISTEMAS DEGRADADOS POR USO AGROPECUARIO, AGROINDUSTRIAL Y ACTIVIDADES EXTRACTIVAS info:eu-repo/semantics/restrictedAccess application/pdf Springer Nature Current Microbiology (2021) |
| spellingShingle | Microorganismos Bradyrhizobium Degradación del Suelo Glifosato Microorganisms Soil Degradation Glyphosate Hernandez Guijarro, Keren De Geronimo, Eduardo Erijman, Leonardo Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title | Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title_full | Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title_fullStr | Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title_full_unstemmed | Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title_short | Glyphosate Biodegradation Potential in Soil Based on Glycine Oxidase Gene (thiO) from Bradyrhizobium |
| title_sort | glyphosate biodegradation potential in soil based on glycine oxidase gene thio from bradyrhizobium |
| topic | Microorganismos Bradyrhizobium Degradación del Suelo Glifosato Microorganisms Soil Degradation Glyphosate |
| url | http://hdl.handle.net/20.500.12123/9141 https://link.springer.com/article/10.1007/s00284-021-02467-z https://doi.org/10.1007/s00284-021-02467-z |
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