Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings

The honeybee Apis mellifera is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxifi...

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Autores principales: Macri, Ivana Noelia, Vásquez, Diego E., Pagano, Eduardo A., Zavala, Jorge Alberto, Farina, Walter Marcelo
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: MDPI 2021
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/9112
https://www.mdpi.com/2075-4450/12/2/163
https://doi.org/10.3390/insects12020163
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author Macri, Ivana Noelia
Vásquez, Diego E.
Pagano, Eduardo A.
Zavala, Jorge Alberto
Farina, Walter Marcelo
author_browse Farina, Walter Marcelo
Macri, Ivana Noelia
Pagano, Eduardo A.
Vásquez, Diego E.
Zavala, Jorge Alberto
author_facet Macri, Ivana Noelia
Vásquez, Diego E.
Pagano, Eduardo A.
Zavala, Jorge Alberto
Farina, Walter Marcelo
author_sort Macri, Ivana Noelia
collection INTA Digital
description The honeybee Apis mellifera is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxification in larvae and adult honeybees from three apiaries located in agricultural environments that differ in their proportion of the crop/wild flora. We evaluated these variables before and after the administration of a mixture of three herbicides during the summer season. The expression of several cytochrome P450 monooxygenases decreased significantly in larvae after post-emergence weed control and showed significant differences between apiaries in the case of honeybee workers. Principal component analysis (PCA) revealed that colonies located in the plot near to a wetland area exhibited a different relative gene expression profile after herbicide application compared with the other plots. Moreover, we found significant positive correlations between pollen collection and the pesticide detoxification genes that discriminated between plots in the PCA. Our results suggest that nutrition may modify herbicide impact on honeybees and that larvae are more harmed than adults in agroecosystems, a factor that will alter the colonies’ population growth at the end of the blooming period.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
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spelling INTA91122025-05-07T11:54:05Z Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings Macri, Ivana Noelia Vásquez, Diego E. Pagano, Eduardo A. Zavala, Jorge Alberto Farina, Walter Marcelo Pest Control Herbicides Intensive Farming Pesticide Application Aplicación de los Pesticidas Apidae Abejas Herbicidas Explotación Agrícola Intensiva Control de Plagas The honeybee Apis mellifera is exposed to agricultural intensification, which leads to an improved reliance upon pesticide use and the reduction of floral diversity. In the present study, we assess the changes in the colony activity and the expression profile of genes involved in xenobiotic detoxification in larvae and adult honeybees from three apiaries located in agricultural environments that differ in their proportion of the crop/wild flora. We evaluated these variables before and after the administration of a mixture of three herbicides during the summer season. The expression of several cytochrome P450 monooxygenases decreased significantly in larvae after post-emergence weed control and showed significant differences between apiaries in the case of honeybee workers. Principal component analysis (PCA) revealed that colonies located in the plot near to a wetland area exhibited a different relative gene expression profile after herbicide application compared with the other plots. Moreover, we found significant positive correlations between pollen collection and the pesticide detoxification genes that discriminated between plots in the PCA. Our results suggest that nutrition may modify herbicide impact on honeybees and that larvae are more harmed than adults in agroecosystems, a factor that will alter the colonies’ population growth at the end of the blooming period. Instituto de Ingeniería Rural Fil: Macri, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Ingeniería Rural; Argentina. Fil: Vásquez, Diego E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Vásquez, Diego E. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Pagano, Eduardo A. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina Fil: Pagano, Eduardo A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Zavala, Jorge A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Zavala, Jorge A. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Bioquímica; Argentina Fil: Farina, Walter M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Farina, Walter M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina Fil: Macri, Ivana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Laboratorio de Insectos Sociales; Argentina Fil: Macri, Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina 2021-04-16T15:48:25Z 2021-04-16T15:48:25Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9112 https://www.mdpi.com/2075-4450/12/2/163 2075-4450 https://doi.org/10.3390/insects12020163 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Insects 12 (2) : 163 (2021)
spellingShingle Pest Control
Herbicides
Intensive Farming
Pesticide Application
Aplicación de los Pesticidas
Apidae
Abejas
Herbicidas
Explotación Agrícola Intensiva
Control de Plagas
Macri, Ivana Noelia
Vásquez, Diego E.
Pagano, Eduardo A.
Zavala, Jorge Alberto
Farina, Walter Marcelo
Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title_full Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title_fullStr Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title_full_unstemmed Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title_short Evaluating the Impact of Post-Emergence Weed Control in Honeybee Colonies Located in Different Agricultural Surroundings
title_sort evaluating the impact of post emergence weed control in honeybee colonies located in different agricultural surroundings
topic Pest Control
Herbicides
Intensive Farming
Pesticide Application
Aplicación de los Pesticidas
Apidae
Abejas
Herbicidas
Explotación Agrícola Intensiva
Control de Plagas
url http://hdl.handle.net/20.500.12123/9112
https://www.mdpi.com/2075-4450/12/2/163
https://doi.org/10.3390/insects12020163
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