Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats
Small ruminant brucellosis is caused by the Gram negative cocci-bacillus Brucella (B.) melitensis, the most virulent Brucella species for humans. In goats and sheep, middle to late-term gestation abortion, stillbirths and the delivery of weak infected offspring are the characteristic clinical signs...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9111 https://www.sciencedirect.com/science/article/pii/S0264410X20314730 https://doi.org/10.1016/j.vaccine.2020.11.033 |
| _version_ | 1855484332893798400 |
|---|---|
| author | Castaño Zubieta, Mirta Raquel Rossetti, Carlos Alberto Garcia-Gonzalez, Daniel G. Maurizio, Estefanía Hensel, Martha E. Rice-Ficht, Allison C. Ficht, Thomas A. Arenas-Gamboa, Angela M. |
| author_browse | Arenas-Gamboa, Angela M. Castaño Zubieta, Mirta Raquel Ficht, Thomas A. Garcia-Gonzalez, Daniel G. Hensel, Martha E. Maurizio, Estefanía Rice-Ficht, Allison C. Rossetti, Carlos Alberto |
| author_facet | Castaño Zubieta, Mirta Raquel Rossetti, Carlos Alberto Garcia-Gonzalez, Daniel G. Maurizio, Estefanía Hensel, Martha E. Rice-Ficht, Allison C. Ficht, Thomas A. Arenas-Gamboa, Angela M. |
| author_sort | Castaño Zubieta, Mirta Raquel |
| collection | INTA Digital |
| description | Small ruminant brucellosis is caused by the Gram negative cocci-bacillus Brucella (B.) melitensis, the most virulent Brucella species for humans. In goats and sheep, middle to late-term gestation abortion, stillbirths and the delivery of weak infected offspring are the characteristic clinical signs of the disease. Vaccination with the currently available Rev. 1 vaccine is the best option to prevent and control the disease, although it is far from ideal. In this study, we investigate the safety of the B. melitensis 16MΔvjbR strain during a 15-month period beginning at vaccination of young goats, impregnation, delivery and lactation. Forty, 4 to 6 months old, healthy female crossbreed goats were randomly divided into four groups (n = 10) and immunized subcutaneously with a single vaccine dose containing 1x109 CFU of B. melitensis 16MΔvjbR delivered in alginate microcapsules or non-encapsulated. Controls received empty capsules or the commercially available Rev.1 vaccine. Seven months post-vaccination, when animals were sexually mature, all goats were naturally bred using brucellosis-free males, and allowed to carry pregnancies to term. Blood samples to assess the humoral immune response were collected throughout the study. At two months post-delivery, all dams and their offspring were euthanized and a necropsy was performed to collect samples for bacteriology and histology. Interestingly, none of the animals that received the vaccine candidate regardless of the formulation exhibited any clinical signs associated with vaccination nor shed the vaccine strain through saliva, vagina or the milk. Gross and histopathologic changes in all nannies and offspring were unremarkable with no evidence of tissue colonization or vertical transmission to fetuses. Altogether, these data demonstrate that vaccination with the mutant strain 16MΔvjbR is safe for use in the non-pregnant primary host. |
| format | Artículo |
| id | INTA9111 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA91112021-04-16T14:50:17Z Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats Castaño Zubieta, Mirta Raquel Rossetti, Carlos Alberto Garcia-Gonzalez, Daniel G. Maurizio, Estefanía Hensel, Martha E. Rice-Ficht, Allison C. Ficht, Thomas A. Arenas-Gamboa, Angela M. Brucella melitensis Brucelosis Vacuna Microencapsulación Goats Brucellosis Vaccines Microencapsulation Caprinos Small ruminant brucellosis is caused by the Gram negative cocci-bacillus Brucella (B.) melitensis, the most virulent Brucella species for humans. In goats and sheep, middle to late-term gestation abortion, stillbirths and the delivery of weak infected offspring are the characteristic clinical signs of the disease. Vaccination with the currently available Rev. 1 vaccine is the best option to prevent and control the disease, although it is far from ideal. In this study, we investigate the safety of the B. melitensis 16MΔvjbR strain during a 15-month period beginning at vaccination of young goats, impregnation, delivery and lactation. Forty, 4 to 6 months old, healthy female crossbreed goats were randomly divided into four groups (n = 10) and immunized subcutaneously with a single vaccine dose containing 1x109 CFU of B. melitensis 16MΔvjbR delivered in alginate microcapsules or non-encapsulated. Controls received empty capsules or the commercially available Rev.1 vaccine. Seven months post-vaccination, when animals were sexually mature, all goats were naturally bred using brucellosis-free males, and allowed to carry pregnancies to term. Blood samples to assess the humoral immune response were collected throughout the study. At two months post-delivery, all dams and their offspring were euthanized and a necropsy was performed to collect samples for bacteriology and histology. Interestingly, none of the animals that received the vaccine candidate regardless of the formulation exhibited any clinical signs associated with vaccination nor shed the vaccine strain through saliva, vagina or the milk. Gross and histopathologic changes in all nannies and offspring were unremarkable with no evidence of tissue colonization or vertical transmission to fetuses. Altogether, these data demonstrate that vaccination with the mutant strain 16MΔvjbR is safe for use in the non-pregnant primary host. Instituto de Patobiología Fil: Castaño Zubieta, Mirta Raquel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Garcia-Gonzalez, Daniel G. Texas A&M University. College of Veterinary Medicine & Biomedical Sciences. Department of Veterinary Pathobiology; Estados Unidos Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hensel, Martha E. Texas A&M University. College of Veterinary Medicine & Biomedical Sciences. Department of Veterinary Pathobiology; Estados Unidos Fil: Rice-Ficht, Allison C. Texas A&M University. College of Medicine. Department of Molecular and Cellular Medicine; Estados Unidos Fil: Ficht, Thomas A. Texas A&M University. College of Veterinary Medicine & Biomedical Sciences. Department of Veterinary Pathobiology; Estados Unidos Fil: Arenas-Gamboa, Angela M. Texas A&M University. College of Veterinary Medicine & Biomedical Sciences. Department of Veterinary Pathobiology; Estados Unidos 2021-04-16T14:43:46Z 2021-04-16T14:43:46Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9111 https://www.sciencedirect.com/science/article/pii/S0264410X20314730 0264-410X https://doi.org/10.1016/j.vaccine.2020.11.033 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Vaccine 39 (3) : 617-625 (Enero 2021) |
| spellingShingle | Brucella melitensis Brucelosis Vacuna Microencapsulación Goats Brucellosis Vaccines Microencapsulation Caprinos Castaño Zubieta, Mirta Raquel Rossetti, Carlos Alberto Garcia-Gonzalez, Daniel G. Maurizio, Estefanía Hensel, Martha E. Rice-Ficht, Allison C. Ficht, Thomas A. Arenas-Gamboa, Angela M. Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title | Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title_full | Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title_fullStr | Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title_full_unstemmed | Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title_short | Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16MDvjbR strain in goats |
| title_sort | evaluation of the safety profile of the vaccine candidate brucella melitensis 16mdvjbr strain in goats |
| topic | Brucella melitensis Brucelosis Vacuna Microencapsulación Goats Brucellosis Vaccines Microencapsulation Caprinos |
| url | http://hdl.handle.net/20.500.12123/9111 https://www.sciencedirect.com/science/article/pii/S0264410X20314730 https://doi.org/10.1016/j.vaccine.2020.11.033 |
| work_keys_str_mv | AT castanozubietamirtaraquel evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT rossetticarlosalberto evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT garciagonzalezdanielg evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT maurizioestefania evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT henselmarthae evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT ricefichtallisonc evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT fichtthomasa evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats AT arenasgamboaangelam evaluationofthesafetyprofileofthevaccinecandidatebrucellamelitensis16mdvjbrstrainingoats |