Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay
Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixode...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9108 https://www.sciencedirect.com/science/article/pii/S2667114X21000169 https://doi.org/10.1016/j.crpvbd.2021.100022 |
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| author | Félix, María L. Muñoz-Leal, Sebastian Carvalho, Luis A. Queirolo, Diego Remesar Alonso, Susana Nava, Santiago Armúa-Fernández, María T. Venzal, José Manuel |
| author_browse | Armúa-Fernández, María T. Carvalho, Luis A. Félix, María L. Muñoz-Leal, Sebastian Nava, Santiago Queirolo, Diego Remesar Alonso, Susana Venzal, José Manuel |
| author_facet | Félix, María L. Muñoz-Leal, Sebastian Carvalho, Luis A. Queirolo, Diego Remesar Alonso, Susana Nava, Santiago Armúa-Fernández, María T. Venzal, José Manuel |
| author_sort | Félix, María L. |
| collection | INTA Digital |
| description | Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health. |
| format | Artículo |
| id | INTA9108 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA91082021-04-16T13:36:44Z Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay Félix, María L. Muñoz-Leal, Sebastian Carvalho, Luis A. Queirolo, Diego Remesar Alonso, Susana Nava, Santiago Armúa-Fernández, María T. Venzal, José Manuel Ehrlichia Genotipos Ixodes Uruguay Marcadores Genéticos Genotypes Genetic Markers Garrapatas Ixodes auritulus Marcadores Moleculares Ticks Ehrlichia are small intracellular Gram-negative bacteria transmitted by ticks. These microorganisms cause ehrlichiosis, a complex of life-threatening emerging zoonoses and diseases of global veterinary relevance. The aim of this study was to investigate the presence of Ehrlichia in free-living Ixodes auritulus collected in Uruguay. Ticks were collected from vegetation in five localities from the southeast and northeast of the country between 2014 and 2017. Detection of Ehrlichia DNA was performed in pools of adults or nymphs grouped according to the collection site and date. A total of 1,548 I. auritulus ticks were collected in four of the five locations sampled. Fragments of three loci (16S rRNA, dsb and groEL) were obtained by PCR, and phylogenies inferred using Bayesian inference analysis for each gene independently. DNA of Ehrlichia spp. was found in 15 out of 42 tick pools. Based on the topology of the phylogenetic trees, our sequences represent two novel genotypes for the genus named as Ehrlichia sp. Serrana and Ehrlichia sp. Laguna Negra. Both genotypes were closely related to Ehrlichia sp. Magellanica, a species detected in Ixodes uriae and Magellanic penguins. Considering that all stages of I. auritulus and I. uriae are parasites of birds, their phylogenetic relationships, and common eco-epidemiological profiles, it is reasonable to state that these genotypes of Ehrlichia spp. may represent a natural group likely associated with birds. Our results constitute the first characterization of Ehrlichia spp. in Uruguay. Future studies on birds reported as hosts for I. auritulus are needed to further understand the epidemiological cycles of both Ehrlichia genotypes in the country. Finally, I. auritulus does not feed on humans, so the two Ehrlichia species reported herein might have no implications in human health. EEA Rafaela Fil: Félix, María L. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Muñoz-Leal, Sebastian. Universidad de Concepción. Facultad de Ciencias Veterinarias. Departamento de Patología y Medicina Preventiva; Chile Fil: Carvalho, Luis A. AgResearch. Grasslands Research Centre; Nueva Zelanda Fil: Queirolo, Diego. Universidad de La República. CENUR Noreste; Uruguay Fil: Remesar Alonso, Susana. Universidade de Santiago de Compostela. Faculty of Veterinary Sciences. Department of Animal Pathology (INVESAGA Group); España Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Armúa-Fernández, María T. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay Fil: Venzal, José Manuel. Universidad de la República. Facultad de Veterinaria. Laboratorio de Vectores y Enfermedades Transmitidas; Uruguay 2021-04-16T13:33:04Z 2021-04-16T13:33:04Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/9108 https://www.sciencedirect.com/science/article/pii/S2667114X21000169 2667-114X https://doi.org/10.1016/j.crpvbd.2021.100022 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Current Research in Parasitology & Vector-Borne Diseases : 100022 (Available online 13 April 2021) |
| spellingShingle | Ehrlichia Genotipos Ixodes Uruguay Marcadores Genéticos Genotypes Genetic Markers Garrapatas Ixodes auritulus Marcadores Moleculares Ticks Félix, María L. Muñoz-Leal, Sebastian Carvalho, Luis A. Queirolo, Diego Remesar Alonso, Susana Nava, Santiago Armúa-Fernández, María T. Venzal, José Manuel Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title | Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title_full | Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title_fullStr | Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title_full_unstemmed | Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title_short | Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay |
| title_sort | molecular characterization of novel ehrlichia genotypes in ixodes auritulus from uruguay |
| topic | Ehrlichia Genotipos Ixodes Uruguay Marcadores Genéticos Genotypes Genetic Markers Garrapatas Ixodes auritulus Marcadores Moleculares Ticks |
| url | http://hdl.handle.net/20.500.12123/9108 https://www.sciencedirect.com/science/article/pii/S2667114X21000169 https://doi.org/10.1016/j.crpvbd.2021.100022 |
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