Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presenc...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/910 https://link.springer.com/article/10.1007%2Fs11259-016-9658-5 https://doi.org/10.1007/s11259-016-9658-5 |
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| author | Salimena, Alessandra P.S. Lange, Carla C. Camussone, Cecilia Signorini, Marcelo Calvinho, Luis Fernando Brito, María A.V.P. Borges, Cristiano A.V. Guimaraes, Alessandro S. Ribeiro, Joao B. Mendonca, Leticia C. Piccoli, Roberta H. |
| author_browse | Borges, Cristiano A.V. Brito, María A.V.P. Calvinho, Luis Fernando Camussone, Cecilia Guimaraes, Alessandro S. Lange, Carla C. Mendonca, Leticia C. Piccoli, Roberta H. Ribeiro, Joao B. Salimena, Alessandra P.S. Signorini, Marcelo |
| author_facet | Salimena, Alessandra P.S. Lange, Carla C. Camussone, Cecilia Signorini, Marcelo Calvinho, Luis Fernando Brito, María A.V.P. Borges, Cristiano A.V. Guimaraes, Alessandro S. Ribeiro, Joao B. Mendonca, Leticia C. Piccoli, Roberta H. |
| author_sort | Salimena, Alessandra P.S. |
| collection | INTA Digital |
| description | Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. |
| format | Artículo |
| id | INTA910 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9102018-06-21T13:01:11Z Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms Salimena, Alessandra P.S. Lange, Carla C. Camussone, Cecilia Signorini, Marcelo Calvinho, Luis Fernando Brito, María A.V.P. Borges, Cristiano A.V. Guimaraes, Alessandro S. Ribeiro, Joao B. Mendonca, Leticia C. Piccoli, Roberta H. Staphylococcus aureus Ganado de Leche Genotipos Fenotipos Leche Dairy Cattle Genotypes Phenotypes Milk Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil. EEA Rafaela Fil: Salimena, Alessandra P.S. EMBRAPA Dairy Cattle Research Center; Brasil. Federal University of Lavras. Food Science Department; Brasil Fil: Lange, Carla C. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Brito, María A.V.P. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Borges, Cristiano A.V. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Guimaraes, Alessandro S. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Ribeiro, Joao B. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Mendonca, Leticia C. EMBRAPA Dairy Cattle Research Center; Brasil Fil: Piccoli, Roberta H. Federal University of Lavras. Food Science Department; Brasil 2017-08-07T13:26:11Z 2017-08-07T13:26:11Z 2016-12 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/910 https://link.springer.com/article/10.1007%2Fs11259-016-9658-5 1573-7446 https://doi.org/10.1007/s11259-016-9658-5 eng info:eu-repo/semantics/restrictedAccess application/pdf Brazil (nation) Veterinary research communications 40 (3-4) : 97-106. (2016) |
| spellingShingle | Staphylococcus aureus Ganado de Leche Genotipos Fenotipos Leche Dairy Cattle Genotypes Phenotypes Milk Salimena, Alessandra P.S. Lange, Carla C. Camussone, Cecilia Signorini, Marcelo Calvinho, Luis Fernando Brito, María A.V.P. Borges, Cristiano A.V. Guimaraes, Alessandro S. Ribeiro, Joao B. Mendonca, Leticia C. Piccoli, Roberta H. Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title | Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title_full | Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title_fullStr | Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title_full_unstemmed | Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title_short | Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms |
| title_sort | genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in staphylococcus aureus isolated from bovine milk collected from brazilian dairy farms |
| topic | Staphylococcus aureus Ganado de Leche Genotipos Fenotipos Leche Dairy Cattle Genotypes Phenotypes Milk |
| url | http://hdl.handle.net/20.500.12123/910 https://link.springer.com/article/10.1007%2Fs11259-016-9658-5 https://doi.org/10.1007/s11259-016-9658-5 |
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