Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition
In order to reproduce, female tephritid fruit flies need both mates for fertilization and fruit for oviposition. Virgin females are prone to mating and approach males, attracted by their pheromones. Mated females, however, may experience an abrupt reduction of mating propensity and prioritise the se...
| Autores principales: | , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9099 https://www.sciencedirect.com/science/article/abs/pii/S0022191021000056 https://doi.org/10.1016/j.jinsphys.2021.104195 |
| _version_ | 1855484330501996544 |
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| author | Devescovi, Francisco Hurtado, Juan Taylor, Phillip W. |
| author_browse | Devescovi, Francisco Hurtado, Juan Taylor, Phillip W. |
| author_facet | Devescovi, Francisco Hurtado, Juan Taylor, Phillip W. |
| author_sort | Devescovi, Francisco |
| collection | INTA Digital |
| description | In order to reproduce, female tephritid fruit flies need both mates for fertilization and fruit for oviposition. Virgin females are prone to mating and approach males, attracted by their pheromones. Mated females, however, may experience an abrupt reduction of mating propensity and prioritise the search for suitable fruit rather than additional mates. Accordingly, mating in fruit flies may induce a switch in olfactory preferences of females from pheromones to fruit stimuli, and this switch may also be an important mediator of mating-induced sexual inhibition. To test for mating-induced switches in olfactory preference of female Queensland fruit fly, Bactrocera tryoni, we used wind tunnel assays to assess attraction of mated and virgin females to (1) male sex pheromone delivered through a perforated glass sphere or (2) an entire fruit. Electroantennogram (EAG) responses were also used to test for mating-induced changes in olfactory sensitivity to pheromones and fruit odours. Pheromones elicited quicker upwind responses in virgin females than in mated females; during the first six minutes of trials more virgin females than mated females were observed in the upwind end of the wind tunnel where pheromone odours were released. Fruit cues, in contrast, elicited stronger association with the upwind end of the wind tunnel in mated females than in virgin females from the fifth minute onwards. Also, mated females were observed on the fruit for longer periods than virgin females. EAG responses to pheromones and fruit odours were similar in virgin and mated females, indicating that changes in preferences are not a consequence of changes in peripheral sensitivity of antennae to odours but instead appear to be mediated by post-receptor processing. Our results show that mating reduces attraction to male-produced pheromones and increases attraction to fruit stimuli in B. tryoni females. We propose that this behavioural switch from mating stimuli to oviposition stimuli is an important mediator of mating-induced sexual inhibition in this species. |
| format | Artículo |
| id | INTA9099 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA90992021-04-15T16:01:10Z Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition Devescovi, Francisco Hurtado, Juan Taylor, Phillip W. Tephritidae Bactrocera tryoni Comportamiento Sexual Olfacción Hembra Feromonas Oviposición Sexual Behaviour Olfaction Females Pheromones Oviposition Sexual Inhibition Inhibición Sexual Mosca de la Fruta In order to reproduce, female tephritid fruit flies need both mates for fertilization and fruit for oviposition. Virgin females are prone to mating and approach males, attracted by their pheromones. Mated females, however, may experience an abrupt reduction of mating propensity and prioritise the search for suitable fruit rather than additional mates. Accordingly, mating in fruit flies may induce a switch in olfactory preferences of females from pheromones to fruit stimuli, and this switch may also be an important mediator of mating-induced sexual inhibition. To test for mating-induced switches in olfactory preference of female Queensland fruit fly, Bactrocera tryoni, we used wind tunnel assays to assess attraction of mated and virgin females to (1) male sex pheromone delivered through a perforated glass sphere or (2) an entire fruit. Electroantennogram (EAG) responses were also used to test for mating-induced changes in olfactory sensitivity to pheromones and fruit odours. Pheromones elicited quicker upwind responses in virgin females than in mated females; during the first six minutes of trials more virgin females than mated females were observed in the upwind end of the wind tunnel where pheromone odours were released. Fruit cues, in contrast, elicited stronger association with the upwind end of the wind tunnel in mated females than in virgin females from the fifth minute onwards. Also, mated females were observed on the fruit for longer periods than virgin females. EAG responses to pheromones and fruit odours were similar in virgin and mated females, indicating that changes in preferences are not a consequence of changes in peripheral sensitivity of antennae to odours but instead appear to be mediated by post-receptor processing. Our results show that mating reduces attraction to male-produced pheromones and increases attraction to fruit stimuli in B. tryoni females. We propose that this behavioural switch from mating stimuli to oviposition stimuli is an important mediator of mating-induced sexual inhibition in this species. Instituto de Genética Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Devescovi, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hurtado, Juan. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Hurtado, Juan. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Taylor, Phillip W. Macquarie University. Applied BioSciences; Australia 2021-04-15T14:55:37Z 2021-04-15T14:55:37Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9099 https://www.sciencedirect.com/science/article/abs/pii/S0022191021000056 1879-1611 https://doi.org/10.1016/j.jinsphys.2021.104195 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Insect Physiology 129 : 104195 (Marzo 2021) |
| spellingShingle | Tephritidae Bactrocera tryoni Comportamiento Sexual Olfacción Hembra Feromonas Oviposición Sexual Behaviour Olfaction Females Pheromones Oviposition Sexual Inhibition Inhibición Sexual Mosca de la Fruta Devescovi, Francisco Hurtado, Juan Taylor, Phillip W. Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title | Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title_full | Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title_fullStr | Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title_full_unstemmed | Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title_short | Mating-induced changes in responses of female Queensland fruit fly to male pheromones and fruit : a mechanism for mating-induced sexual inhibition |
| title_sort | mating induced changes in responses of female queensland fruit fly to male pheromones and fruit a mechanism for mating induced sexual inhibition |
| topic | Tephritidae Bactrocera tryoni Comportamiento Sexual Olfacción Hembra Feromonas Oviposición Sexual Behaviour Olfaction Females Pheromones Oviposition Sexual Inhibition Inhibición Sexual Mosca de la Fruta |
| url | http://hdl.handle.net/20.500.12123/9099 https://www.sciencedirect.com/science/article/abs/pii/S0022191021000056 https://doi.org/10.1016/j.jinsphys.2021.104195 |
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