Karyotypic analyses and genomic affinity among Argentinean species of Passiflora
The genus Passiflora (Passifloraceae) has more than 500 species, nineteen are native to Argentina. By their austral distribution, these species were included in breeding programs to obtain ornamental varieties tolerant to low temperatures. The aim of this work was to know the cytogenetic characteris...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2021
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| Online Access: | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100286&tlng=en https://doi.org/10.1590/2175-7860202071110 |
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| author | Bugallo, Veronica Lucia Realini, María Florencia Facciuto, Gabriela Rosa Poggio, Lidia |
| author_browse | Bugallo, Veronica Lucia Facciuto, Gabriela Rosa Poggio, Lidia Realini, María Florencia |
| author_facet | Bugallo, Veronica Lucia Realini, María Florencia Facciuto, Gabriela Rosa Poggio, Lidia |
| author_sort | Bugallo, Veronica Lucia |
| collection | INTA Digital |
| description | The genus Passiflora (Passifloraceae) has more than 500 species, nineteen are native to Argentina. By their austral distribution, these species were included in breeding programs to obtain ornamental varieties tolerant to low temperatures. The aim of this work was to know the cytogenetic characteristics of Passiflora genotypes present in a working collection, as an indispensable knowledge for the development of a breeding plan. Chromosomal preparations were performed and karyotypic characteristics, rDNA sites by FISH and affinity among subgenera by GISH were studied. Chromosome counts in fourteen Argentinean species confirmed the basic chromosome numbers previously published: x = 6 for subgenus Decaloba, x = 9 for subgenus Passiflora and x = 10 for Dysosmia. The karyotypic parameters (karyotype, haploid chromosome length and asymmetry indices) and genomic affinities among the subgenera, clarify most of the chromosomal evolution of the genus. The results obtained strongly suggest that the basic number x = 6 would be the original one and that x = 9 was originated by processes of polyploidy and descendent dysploidy. Since subgenus Passiflora possesses the largest genomes, it is postulated that evolutionary process leading to x = 9 was accompanied by unequal distribution of non-coding repetitive DNA, mainly transposable elements. These processes could explain the asymmetrical karyotypes of species of subgenus Passiflora. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9093 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| publisherStr | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | dspace |
| spelling | INTA90932021-04-15T11:47:42Z Karyotypic analyses and genomic affinity among Argentinean species of Passiflora Bugallo, Veronica Lucia Realini, María Florencia Facciuto, Gabriela Rosa Poggio, Lidia Karyotypes Genetics Cariotipos Passiflora Genética Chromosome Numbers In Situ Hybridization Native Passiflora to Argentina Ornamental Plant Breeding Números de Cromosomas Hibridación in Situ Passiflora nativa de Argentina Mejoramiento de Plantas Ornamentales The genus Passiflora (Passifloraceae) has more than 500 species, nineteen are native to Argentina. By their austral distribution, these species were included in breeding programs to obtain ornamental varieties tolerant to low temperatures. The aim of this work was to know the cytogenetic characteristics of Passiflora genotypes present in a working collection, as an indispensable knowledge for the development of a breeding plan. Chromosomal preparations were performed and karyotypic characteristics, rDNA sites by FISH and affinity among subgenera by GISH were studied. Chromosome counts in fourteen Argentinean species confirmed the basic chromosome numbers previously published: x = 6 for subgenus Decaloba, x = 9 for subgenus Passiflora and x = 10 for Dysosmia. The karyotypic parameters (karyotype, haploid chromosome length and asymmetry indices) and genomic affinities among the subgenera, clarify most of the chromosomal evolution of the genus. The results obtained strongly suggest that the basic number x = 6 would be the original one and that x = 9 was originated by processes of polyploidy and descendent dysploidy. Since subgenus Passiflora possesses the largest genomes, it is postulated that evolutionary process leading to x = 9 was accompanied by unequal distribution of non-coding repetitive DNA, mainly transposable elements. These processes could explain the asymmetrical karyotypes of species of subgenus Passiflora. El género Passiflora (Passifloraceae) cuenta con más de 500 especies, diecinueve de ellas son nativas de Argentina. Por su distribución austral, estas especies fueron incluidas en un plan de mejoramiento para obtener variedades ornamentales tolerantes a bajas temperaturas. El objetivo de este trabajo fue conocer las características citogenéticas de los genotipos de Passiflora en la colección de trabajo como conocimiento indispensable para el desarrollo de un plan de mejoramiento. Se realizaron preparados cromosómicos y se estudiaron las características cariotípicas, regiones ADNr por FISH y afinidad genómica entre subgéneros por GISH. Los recuentos cromosómicos realizados en catorce especies argentinas confirmaron los previamente publicados: x = 6 para el subgénero Decaloba, x = 9 para el subgénero Passiflora y x = 10 para el subgénero Dysosmia. Los parámetros cariotípicos (cariotipos, longitud del genoma haploide e índices de asimetría) y las afinidades genómicas analizadas por GISH entre las especies de los tres subgéneros, clarifican la mayor parte de la evolución cromosómica del género. Los resultados obtenidos sugieren que el número básico x = 6 sería el original y que x = 9 se habría originado por procesos de poliploidía y disploidía descendente. Debido a que las especies del subgénero Passiflora poseen los genomas más largos, se postula que el proceso evolutivo que llevó de x = 10 a x = 9 fue acompañado por una distribución desigual en los brazos cromosómicos de ADN repetitivo no codificante, principalmente elementos transponibles. Estos procesos explicarían la asimetría cariotípica del subgénero Passiflora. Fil: Bugallo, Veronica Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina. Universidad de Buenos Aires. Facultad de Agronomía; Argentina Fil: Realini, María Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ecología, Genética y Evolución; Argentina Fil: Facciuto, Gabriela Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Floricultura; Argentina Fil: Poggio, Lidia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento Ecología, Genética y Evolución. Laboratorio de Citogenética y Evolución; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ecología, Genética y Evolución; Argentina 2021-04-15T11:24:00Z 2021-04-15T11:24:00Z 2020-11-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9093 https://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100286&tlng=en 0370-6583 2175-7860 https://doi.org/10.1590/2175-7860202071110 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Instituto de Pesquisas Jardim Botânico do Rio de Janeiro Rodriguésia 71 : e03532018. (2020) |
| spellingShingle | Karyotypes Genetics Cariotipos Passiflora Genética Chromosome Numbers In Situ Hybridization Native Passiflora to Argentina Ornamental Plant Breeding Números de Cromosomas Hibridación in Situ Passiflora nativa de Argentina Mejoramiento de Plantas Ornamentales Bugallo, Veronica Lucia Realini, María Florencia Facciuto, Gabriela Rosa Poggio, Lidia Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title | Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title_full | Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title_fullStr | Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title_full_unstemmed | Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title_short | Karyotypic analyses and genomic affinity among Argentinean species of Passiflora |
| title_sort | karyotypic analyses and genomic affinity among argentinean species of passiflora |
| topic | Karyotypes Genetics Cariotipos Passiflora Genética Chromosome Numbers In Situ Hybridization Native Passiflora to Argentina Ornamental Plant Breeding Números de Cromosomas Hibridación in Situ Passiflora nativa de Argentina Mejoramiento de Plantas Ornamentales |
| url | https://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602020000100286&tlng=en https://doi.org/10.1590/2175-7860202071110 |
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