Role of PhoPR in the response to stress of Mycobacterium bovis
PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene ex...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9089 https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X https://doi.org/10.1016/j.cimid.2020.101593 |
| _version_ | 1855484328783380480 |
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| author | Garcia, Elizabeth Andrea Blanco, Federico Carlos Klepp, Laura Ines Pazos, Adriana Alejandra McNeil, Michael Jackson, Mary Bigi, Fabiana |
| author_browse | Bigi, Fabiana Blanco, Federico Carlos Garcia, Elizabeth Andrea Jackson, Mary Klepp, Laura Ines McNeil, Michael Pazos, Adriana Alejandra |
| author_facet | Garcia, Elizabeth Andrea Blanco, Federico Carlos Klepp, Laura Ines Pazos, Adriana Alejandra McNeil, Michael Jackson, Mary Bigi, Fabiana |
| author_sort | Garcia, Elizabeth Andrea |
| collection | INTA Digital |
| description | PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis. |
| format | Artículo |
| id | INTA9089 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA90892021-04-14T16:43:09Z Role of PhoPR in the response to stress of Mycobacterium bovis Garcia, Elizabeth Andrea Blanco, Federico Carlos Klepp, Laura Ines Pazos, Adriana Alejandra McNeil, Michael Jackson, Mary Bigi, Fabiana Mycobacterium bovis Estres Virulencia Infeccion por Mycobacterium bovis Amoníaco Stress Virulence Mycobacterium bovis Infections Tuberculosis Ammonia PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis. Instituto de Biotecnología Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pazos, Adriana Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina Fil: McNeil, Michael. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos Fil: Jackson, Mary. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-04-14T16:33:03Z 2021-04-14T16:33:03Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9089 https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X 1878-1667 https://doi.org/10.1016/j.cimid.2020.101593 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative Immunology, Microbiology and Infectious Diseases 74 : 101593 (Febrero 2021) |
| spellingShingle | Mycobacterium bovis Estres Virulencia Infeccion por Mycobacterium bovis Amoníaco Stress Virulence Mycobacterium bovis Infections Tuberculosis Ammonia Garcia, Elizabeth Andrea Blanco, Federico Carlos Klepp, Laura Ines Pazos, Adriana Alejandra McNeil, Michael Jackson, Mary Bigi, Fabiana Role of PhoPR in the response to stress of Mycobacterium bovis |
| title | Role of PhoPR in the response to stress of Mycobacterium bovis |
| title_full | Role of PhoPR in the response to stress of Mycobacterium bovis |
| title_fullStr | Role of PhoPR in the response to stress of Mycobacterium bovis |
| title_full_unstemmed | Role of PhoPR in the response to stress of Mycobacterium bovis |
| title_short | Role of PhoPR in the response to stress of Mycobacterium bovis |
| title_sort | role of phopr in the response to stress of mycobacterium bovis |
| topic | Mycobacterium bovis Estres Virulencia Infeccion por Mycobacterium bovis Amoníaco Stress Virulence Mycobacterium bovis Infections Tuberculosis Ammonia |
| url | http://hdl.handle.net/20.500.12123/9089 https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X https://doi.org/10.1016/j.cimid.2020.101593 |
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