Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor
The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displa...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9088 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13290 https://doi.org/10.1111/ppa.13290 |
| _version_ | 1855036151773003776 |
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| author | Barrios Baron, Maria Pilar Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Nahirñak, Vanesa Almasia, Natalia Ines Vazquez Rovere, Cecilia Distefano, Ana Julia |
| author_browse | Agrofoglio, Yamila Carla Almasia, Natalia Ines Barrios Baron, Maria Pilar Delfosse, Veronica Cecilia Distefano, Ana Julia Nahirñak, Vanesa Vazquez Rovere, Cecilia |
| author_facet | Barrios Baron, Maria Pilar Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Nahirñak, Vanesa Almasia, Natalia Ines Vazquez Rovere, Cecilia Distefano, Ana Julia |
| author_sort | Barrios Baron, Maria Pilar |
| collection | INTA Digital |
| description | The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9088 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA90882021-04-14T16:05:55Z Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor Barrios Baron, Maria Pilar Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Nahirñak, Vanesa Almasia, Natalia Ines Vazquez Rovere, Cecilia Distefano, Ana Julia Potato Leaf Roll Virus Immunosuppressants Luteovirus del Enrollado de Patata Solanum tuberosum Inmunodepresores Argonaute Argonauta The potato leafroll virus (PLRV) P0 protein (P0PL) is a suppressor of RNA silencing. In this study, we showed that P0 protein from an Argentinian isolate of PLRV (P0PL‐Ar) has an additional activity not described for other PLRV or P0 proteins from poleroviruses. Besides reporting that P0PL‐Ar displays both local and systemic silencing suppressor activity, we demonstrated, for the first time, that P0PL‐Ar impedes accumulation of dsRNA‐derived siRNAs. We also showed that P0PL‐Ar interacts with Solanum tuberosum SKP1 orthologue (StSKP1) and triggers destabilization of ARGONAUTE 1 (AGO1) and that these actions are mediated by the F‐box‐like domain. A mutant in the GW/WG motif within the P0PL‐Ar F‐box‐like motif lost the suppression activity, the interaction with StSKP1 and abolished AGO1 decay. Interestingly, a mutant in the L76/P77 residues within the P0PL‐Ar F‐box‐like motif, which lost the suppression activity and the interaction with StSKP1, retained the capacity to enable AGO1 decay. Thus, unlike other P0 proteins of previously characterized poleroviruses, P0PL‐Ar seems to have a dual activity, according to the findings of this study. This protein would act at both an upstream and a downstream step of the RNA silencing pathway: upstream of Dicer‐like enzyme (DCL)‐mediated primary siRNA production and downstream at the RNA‐induced silencing complex (RISC) complex level. Our results contribute to the understanding of the different ways PLRV P0 proteins function as silencing suppressors. Instituto de Biotecnología Fil: Barrios Baron, Maria Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Barrios Baron, Maria Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delfosse, Veronica Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Delfosse, Veronica Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delfosse, Veronica Cecilia. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina Fil: Agrofoglio, Yamila Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Agrofoglio, Yamila Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nahirñak, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Almasia, Natalia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Vazquez Rovere, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Distefano, Ana Julia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Distefano, Ana Julia. Universidad Nacional de Luján (UNLU); Argentina 2021-04-14T15:57:32Z 2021-04-14T15:57:32Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9088 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13290 1365-3059 https://doi.org/10.1111/ppa.13290 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001/2019-PD-E4-I085-001/AR./Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Pathology 70 (2) : 259-274 (Febrero 2021) |
| spellingShingle | Potato Leaf Roll Virus Immunosuppressants Luteovirus del Enrollado de Patata Solanum tuberosum Inmunodepresores Argonaute Argonauta Barrios Baron, Maria Pilar Delfosse, Veronica Cecilia Agrofoglio, Yamila Carla Nahirñak, Vanesa Almasia, Natalia Ines Vazquez Rovere, Cecilia Distefano, Ana Julia Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title | Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title_full | Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title_fullStr | Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title_full_unstemmed | Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title_short | Argentinian potato leafroll virus P0 protein : Novel activities for a previously known suppressor |
| title_sort | argentinian potato leafroll virus p0 protein novel activities for a previously known suppressor |
| topic | Potato Leaf Roll Virus Immunosuppressants Luteovirus del Enrollado de Patata Solanum tuberosum Inmunodepresores Argonaute Argonauta |
| url | http://hdl.handle.net/20.500.12123/9088 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13290 https://doi.org/10.1111/ppa.13290 |
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