Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way
Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Verlag
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9082 https://link.springer.com/article/10.1007/s00299-020-02617-w https://doi.org/10.1007/s00299-020-02617-w |
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| author | Varela, Anabella Ibañez, Verónica Noé Alonso, Rodrigo Zavallo, Diego Asurmendi, Sebastian Gomez Talquenca, Gonzalo Sebastián Marfil, Carlos Federico Berli, Federico Javier |
| author_browse | Alonso, Rodrigo Asurmendi, Sebastian Berli, Federico Javier Gomez Talquenca, Gonzalo Sebastián Ibañez, Verónica Noé Marfil, Carlos Federico Varela, Anabella Zavallo, Diego |
| author_facet | Varela, Anabella Ibañez, Verónica Noé Alonso, Rodrigo Zavallo, Diego Asurmendi, Sebastian Gomez Talquenca, Gonzalo Sebastián Marfil, Carlos Federico Berli, Federico Javier |
| author_sort | Varela, Anabella |
| collection | INTA Digital |
| description | Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9082 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Verlag |
| publisherStr | Springer Verlag |
| record_format | dspace |
| spelling | INTA90822024-04-22T10:24:06Z Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way Varela, Anabella Ibañez, Verónica Noé Alonso, Rodrigo Zavallo, Diego Asurmendi, Sebastian Gomez Talquenca, Gonzalo Sebastián Marfil, Carlos Federico Berli, Federico Javier Epigenetics Methylation Phenotypic Plasticity Vineyards Epigenético Metilación Plasticidad Fenotípica Vitis vinifera Viña Malbec Clonal selection and vegetative propagation determine low genetic variability in grapevine cultivars, although it is common to observe diverse phenotypes. Environmental signals may induce epigenetic changes altering gene expression and phenotype. The range of phenotypes that a genotype expresses in different environments is known as phenotypic plasticity. DNA methylation is the most studied epigenetic mechanism, but only few works evaluated this novel source of variability in grapevines. In the present study, we analyzed the effects on phenotypic traits and epigenome of three Vitis vinifera cv. Malbec clones cultivated in two contrasting vineyards of Mendoza, Argentina. Anonymous genome regions were analyzed using methylation-sensitive amplified polymorphism (MSAP) markers. Clone-dependent phenotypic and epigenetic variability between vineyards were found. The clone that presented the clearer MSAP differentiation between vineyards was selected and analyzed through reduced representation bisulfite sequencing. Twenty-nine differentially methylated regions between vineyards were identified and associated to genes and/or promoters. We discuss about a group of genes related to hormones homeostasis and sensing that could provide a hint of the epigenetic role in the determination of the different phenotypes observed between vineyards and conclude that DNA methylation has an important role in the phenotypic plasticity and that epigenetic modulation is clone-dependent. Instituto de Biotecnología Fil: Varela, Anabella. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Varela, Anabella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ibañez, Verónica N. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Ibañez, Verónica N. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alonso, Rodrigo. Bodega Catena Zapata; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gomez Talquenca, Gonzalo Sebastián. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina Fil: Marfil, Carlos Federico. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Marfil, Carlos Federico. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berli, Federico Javier. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Berli, Federico Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-04-14T11:01:39Z 2021-04-14T11:01:39Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9082 https://link.springer.com/article/10.1007/s00299-020-02617-w 1432-203X https://doi.org/10.1007/s00299-020-02617-w eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Verlag Plant Cell Reports 40 (1) : 111-125 (Enero 2021) |
| spellingShingle | Epigenetics Methylation Phenotypic Plasticity Vineyards Epigenético Metilación Plasticidad Fenotípica Vitis vinifera Viña Malbec Varela, Anabella Ibañez, Verónica Noé Alonso, Rodrigo Zavallo, Diego Asurmendi, Sebastian Gomez Talquenca, Gonzalo Sebastián Marfil, Carlos Federico Berli, Federico Javier Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title | Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title_full | Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title_fullStr | Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title_full_unstemmed | Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title_short | Vineyard environments influence Malbec grapevine phenotypic traits and DNA methylation patterns in a clone‑dependent way |
| title_sort | vineyard environments influence malbec grapevine phenotypic traits and dna methylation patterns in a clone dependent way |
| topic | Epigenetics Methylation Phenotypic Plasticity Vineyards Epigenético Metilación Plasticidad Fenotípica Vitis vinifera Viña Malbec |
| url | http://hdl.handle.net/20.500.12123/9082 https://link.springer.com/article/10.1007/s00299-020-02617-w https://doi.org/10.1007/s00299-020-02617-w |
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