Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates
Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was t...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Wolters Kluwer Medknow
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9081 https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano https://doi.org/10.4103/ijmy.ijmy_229_20 |
| _version_ | 1855036149169389568 |
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| author | Moyano, Roberto Damián Imperiale, Belen Rocio Romero, Magalí Santangelo, María De La Paz Alvarado Pinedo, Maria Fiorella Travería, Gabriel Romano, Maria Isabel |
| author_browse | Alvarado Pinedo, Maria Fiorella Imperiale, Belen Rocio Moyano, Roberto Damián Romano, Maria Isabel Romero, Magalí Santangelo, María De La Paz Travería, Gabriel |
| author_facet | Moyano, Roberto Damián Imperiale, Belen Rocio Romero, Magalí Santangelo, María De La Paz Alvarado Pinedo, Maria Fiorella Travería, Gabriel Romano, Maria Isabel |
| author_sort | Moyano, Roberto Damián |
| collection | INTA Digital |
| description | Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9081 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wolters Kluwer Medknow |
| publisherStr | Wolters Kluwer Medknow |
| record_format | dspace |
| spelling | INTA90812021-04-14T10:58:46Z Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates Moyano, Roberto Damián Imperiale, Belen Rocio Romero, Magalí Santangelo, María De La Paz Alvarado Pinedo, Maria Fiorella Travería, Gabriel Romano, Maria Isabel Mycobacterium avium subsp. paratuberculosis Infección por Mycobacterium avium Variación Genética Loci de Rasgos Cuantitativos Aislamiento Genético Mycobacterium avium Infections Genetic Variation Quantitative Trait Loci Genetic Isolation Background: Paratuberculosis is an enteric disease caused by Mycobacterium avium sp. paratuberculosis (MAP) that affects mainly ruminant producing losses to the livestock industry. Many molecular epidemiological methods have been used to discriminate MAP isolates. Method: The aim of this study was to describe the genetic diversity of the Argentinean MAP isolates using a combination of two molecular systems, the mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) (“automated and “non-automated”) and the multi-locus short-sequence repeat (MLSSR) system. Results: Thirty-two isolates were identified as MAP of C type by IS900 polymerase chain reaction (PCA) and IS1311 PCA-restriction enzyme analysis. The main patterns found by both MIRU-VNTR systems were INMV1 (54.5%), INMV2 (24.2%) and INMV11 (9.1%). The INMV5, INMV8 and INMV16 were represented with one isolate each (3.0%). Only 4 MIRU-VNTR loci were polymorphic. Conclusion: Those isolates sharing the same INMV patterns were analyzed by MLSSR, being locus 2 the most polymorphic one showing isolates with 9, 10, 11, and more than 11 “G” repeats. Besides, the global discriminatory power among isolates could be increased using both techniques. Based on these results, a short version of the “automated” MIRU-VNTR could be used as a screening tool to group isolates genetically related and subsequently perform the SSR using locus 2 on those isolates sharing the same INMV pattern. Instituto de Biotecnología Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Imperiale, Belen Rocio. Academia Nacional de Medicina. Instituto de Medicina Experimental (IMEX); Argentina Fil: Imperiale, Belen Rocio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Magalí. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarado Pinedo, Maria Fiorella. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Travería, Gabriel. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigación Veterinaria (CEDIVE); Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-04-14T10:45:47Z 2021-04-14T10:45:47Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9081 https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano 2212-5531 https://doi.org/10.4103/ijmy.ijmy_229_20 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wolters Kluwer Medknow International Journal of Mycobacteriology 10 (1) : 51-59 (Marzo 2021) |
| spellingShingle | Mycobacterium avium subsp. paratuberculosis Infección por Mycobacterium avium Variación Genética Loci de Rasgos Cuantitativos Aislamiento Genético Mycobacterium avium Infections Genetic Variation Quantitative Trait Loci Genetic Isolation Moyano, Roberto Damián Imperiale, Belen Rocio Romero, Magalí Santangelo, María De La Paz Alvarado Pinedo, Maria Fiorella Travería, Gabriel Romano, Maria Isabel Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title | Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title_full | Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title_fullStr | Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title_full_unstemmed | Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title_short | Genetic diversity of Mycobacterium avium sp. Paratuberculosis by mycobacterial interspersed repetitive unit–variable number tandem repeat and multi‑locus short‑sequence repeat one‑sentence summary : genetic diversity of Mycobacterium avium sp. Paratuberculosis isolates |
| title_sort | genetic diversity of mycobacterium avium sp paratuberculosis by mycobacterial interspersed repetitive unit variable number tandem repeat and multi locus short sequence repeat one sentence summary genetic diversity of mycobacterium avium sp paratuberculosis isolates |
| topic | Mycobacterium avium subsp. paratuberculosis Infección por Mycobacterium avium Variación Genética Loci de Rasgos Cuantitativos Aislamiento Genético Mycobacterium avium Infections Genetic Variation Quantitative Trait Loci Genetic Isolation |
| url | http://hdl.handle.net/20.500.12123/9081 https://www.ijmyco.org/article.asp?issn=2212-5531;year=2021;volume=10;issue=1;spage=51;epage=59;aulast=Moyano https://doi.org/10.4103/ijmy.ijmy_229_20 |
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