Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth
Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/9079 https://www.sciencedirect.com/science/article/abs/pii/S0165242721000416 https://doi.org/10.1016/j.vetimm.2021.110223 |
| _version_ | 1855484325522309120 |
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| author | Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto |
| author_browse | Colato, Claudio Dunleavy, Mariana Victoria Maurizio, Estefanía Rossetti, Carlos Alberto Rossi, Ursula Trangoni, Marcos David |
| author_facet | Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto |
| author_sort | Maurizio, Estefanía |
| collection | INTA Digital |
| description | Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats. |
| format | Artículo |
| id | INTA9079 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA90792021-04-13T15:44:11Z Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats. Instituto de Patobiología Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dunleavy, Mariana Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Dunleavy, Mariana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colato, Claudio. Cabaña Nuevo Milenium; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-04-13T15:33:04Z 2021-04-13T15:33:04Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9079 https://www.sciencedirect.com/science/article/abs/pii/S0165242721000416 0165-2427 https://doi.org/10.1016/j.vetimm.2021.110223 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 234 : 110223 (Abril 2021) |
| spellingShingle | Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title | Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title_full | Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title_fullStr | Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title_full_unstemmed | Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title_short | Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
| title_sort | characterization of innate immune response to brucella melitensis infection in goats with permissive or restrictive phenotype for brucella intramacrophagic growth |
| topic | Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders |
| url | http://hdl.handle.net/20.500.12123/9079 https://www.sciencedirect.com/science/article/abs/pii/S0165242721000416 https://doi.org/10.1016/j.vetimm.2021.110223 |
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