Gene expression and in vitro replication of bovine gammaherpesvirus type 4
In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions rem...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Springer Verlag
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9066 https://link.springer.com/article/10.1007/s00705-020-04898-8 https://doi.org/10.1007/s00705-020-04898-8 |
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| author | Romeo, Florencia Louge Uriarte, Enrique Leopoldo González Altamiranda, Erika Delgado, Santiago Germán Pereyra, Susana Beatriz Moran, P. Odeon, Anselmo Carlos Perez, Sandra Verna, Andrea Elizabeth |
| author_browse | Delgado, Santiago Germán González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Moran, P. Odeon, Anselmo Carlos Pereyra, Susana Beatriz Perez, Sandra Romeo, Florencia Verna, Andrea Elizabeth |
| author_facet | Romeo, Florencia Louge Uriarte, Enrique Leopoldo González Altamiranda, Erika Delgado, Santiago Germán Pereyra, Susana Beatriz Moran, P. Odeon, Anselmo Carlos Perez, Sandra Verna, Andrea Elizabeth |
| author_sort | Romeo, Florencia |
| collection | INTA Digital |
| description | In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies. |
| format | Artículo |
| id | INTA9066 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Verlag |
| publisherStr | Springer Verlag |
| record_format | dspace |
| spelling | INTA90662021-04-12T16:09:19Z Gene expression and in vitro replication of bovine gammaherpesvirus type 4 Romeo, Florencia Louge Uriarte, Enrique Leopoldo González Altamiranda, Erika Delgado, Santiago Germán Pereyra, Susana Beatriz Moran, P. Odeon, Anselmo Carlos Perez, Sandra Verna, Andrea Elizabeth Herpes Virus Bovino Replicación Viral Expresión Génica In Vitro Enfermedades de los Animales Bovine Herpesvirus Gene Expression Animal Diseases Viral Replication In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies. EEA Balcarce Fil: Romeo, Florencia. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Fil: Romeo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Gonzalez Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Morán, P. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. 2021-04-12T15:57:55Z 2021-04-12T15:57:55Z 2021-02-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9066 https://link.springer.com/article/10.1007/s00705-020-04898-8 1432-8798 (online) 0304-8608 (print) https://doi.org/10.1007/s00705-020-04898-8 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Verlag Archives of Virology 166 : 535–544 (2021) |
| spellingShingle | Herpes Virus Bovino Replicación Viral Expresión Génica In Vitro Enfermedades de los Animales Bovine Herpesvirus Gene Expression Animal Diseases Viral Replication Romeo, Florencia Louge Uriarte, Enrique Leopoldo González Altamiranda, Erika Delgado, Santiago Germán Pereyra, Susana Beatriz Moran, P. Odeon, Anselmo Carlos Perez, Sandra Verna, Andrea Elizabeth Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title | Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title_full | Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title_fullStr | Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title_full_unstemmed | Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title_short | Gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| title_sort | gene expression and in vitro replication of bovine gammaherpesvirus type 4 |
| topic | Herpes Virus Bovino Replicación Viral Expresión Génica In Vitro Enfermedades de los Animales Bovine Herpesvirus Gene Expression Animal Diseases Viral Replication |
| url | http://hdl.handle.net/20.500.12123/9066 https://link.springer.com/article/10.1007/s00705-020-04898-8 https://doi.org/10.1007/s00705-020-04898-8 |
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