The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Plos One
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/9040 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242526 https://doi.org/10.1371/journal.pone.0242526 |
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| author | Caspe, Sergio Gaston Livingstone, Morag Frew, David Aitchison, Kevin Wattegedera, Sean Ranjan Entrican, Gary Palarea-Albaladejo, Javier McNeilly, Tom Nathan Milne, Elspeth Sargison, Neil Donald Chianini, Francesca Longbottom, David |
| author_browse | Aitchison, Kevin Caspe, Sergio Gaston Chianini, Francesca Entrican, Gary Frew, David Livingstone, Morag Longbottom, David McNeilly, Tom Nathan Milne, Elspeth Palarea-Albaladejo, Javier Sargison, Neil Donald Wattegedera, Sean Ranjan |
| author_facet | Caspe, Sergio Gaston Livingstone, Morag Frew, David Aitchison, Kevin Wattegedera, Sean Ranjan Entrican, Gary Palarea-Albaladejo, Javier McNeilly, Tom Nathan Milne, Elspeth Sargison, Neil Donald Chianini, Francesca Longbottom, David |
| author_sort | Caspe, Sergio Gaston |
| collection | INTA Digital |
| description | Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9040 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Plos One |
| publisherStr | Plos One |
| record_format | dspace |
| spelling | INTA90402024-08-05T11:25:14Z The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection Caspe, Sergio Gaston Livingstone, Morag Frew, David Aitchison, Kevin Wattegedera, Sean Ranjan Entrican, Gary Palarea-Albaladejo, Javier McNeilly, Tom Nathan Milne, Elspeth Sargison, Neil Donald Chianini, Francesca Longbottom, David Ganado Bovino Enfermedades de los Animales Chlamydia Chlamydophila abortus Control de Enfermedades Vacuna Placenta Cattle Animal Diseases Diseases Control Vaccines Chlamydia abortus Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals. EEA Mercedes Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido Fil: Caspe, Sergio Gaston. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido Fil: Frew, David. Moredun Research Institute; Reino Unido Fil: Aitchison, Kevin. Moredun Research Institute; Reino Unido Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido Fil: Entrican, Gary. Moredun Research Institute; Reino Unido Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido Fil: Longbottom, David. Moredun Research Institute; Reino Unido 2021-04-07T13:26:09Z 2021-04-07T13:26:09Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9040 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242526 1932-6203 https://doi.org/10.1371/journal.pone.0242526 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos One PLoS ONE 15 (11) : e0242526. (2020) |
| spellingShingle | Ganado Bovino Enfermedades de los Animales Chlamydia Chlamydophila abortus Control de Enfermedades Vacuna Placenta Cattle Animal Diseases Diseases Control Vaccines Chlamydia abortus Caspe, Sergio Gaston Livingstone, Morag Frew, David Aitchison, Kevin Wattegedera, Sean Ranjan Entrican, Gary Palarea-Albaladejo, Javier McNeilly, Tom Nathan Milne, Elspeth Sargison, Neil Donald Chianini, Francesca Longbottom, David The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title | The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title_full | The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title_fullStr | The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title_full_unstemmed | The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title_short | The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| title_sort | 1b vaccine strain of chlamydia abortus produces placental pathology indistinguishable from a wild type infection |
| topic | Ganado Bovino Enfermedades de los Animales Chlamydia Chlamydophila abortus Control de Enfermedades Vacuna Placenta Cattle Animal Diseases Diseases Control Vaccines Chlamydia abortus |
| url | http://hdl.handle.net/20.500.12123/9040 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242526 https://doi.org/10.1371/journal.pone.0242526 |
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