Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 i...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 https://doi.org/10.1016/j.idairyj.2021.105027 |
| _version_ | 1855036137887760384 |
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| author | Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego |
| author_browse | Amadio, Ariel Galliani, Valentina Irazoqui, Jose Matias Mercanti, Diego Pujato, Silvina A. Quiberoni, Andrea |
| author_facet | Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego |
| author_sort | Pujato, Silvina A. |
| collection | INTA Digital |
| description | A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9014 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA90142021-03-31T16:43:29Z Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections. EEA Rafaela Fil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina 2021-03-31T16:33:53Z 2021-03-31T16:33:53Z 2021-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 0958-6946 https://doi.org/10.1016/j.idairyj.2021.105027 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier International Dairy Journal 118 : 105027 (July 2021) |
| spellingShingle | Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title | Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title_full | Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title_fullStr | Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title_full_unstemmed | Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title_short | Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
| title_sort | analysis of crispr systems of types ii a i e and i c in strains of lacticaseibacillus |
| topic | Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems |
| url | http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 https://doi.org/10.1016/j.idairyj.2021.105027 |
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