Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species
Differential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/9008 https://link.springer.com/article/10.1007/s10265-021-01279-3 https://doi.org/10.1007/s10265-021-01279-3 |
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| author | Cascales, Jimena Acevedo, Raúl Maximiliano Paiva, Daniela Ivana Gottlieb, Alexandra Marina |
| author_browse | Acevedo, Raúl Maximiliano Cascales, Jimena Gottlieb, Alexandra Marina Paiva, Daniela Ivana |
| author_facet | Cascales, Jimena Acevedo, Raúl Maximiliano Paiva, Daniela Ivana Gottlieb, Alexandra Marina |
| author_sort | Cascales, Jimena |
| collection | INTA Digital |
| description | Differential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual differentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a first step towards the development of an early sexing system, we searched for promising molecular markers. This was assessed through Methylation Sensitive Amplified Polymorphism (MSAP) and Amplified Fragment Length Polymorphism on cDNA (cDNA-AFLP) techniques, applying discriminant multivariate analyses, and bioinformatic characterization of differential fragments. A significant positive correlation was found between epigenetic and indirect ‘genetic’ information for both species, indicating influence of the genetic background on the epigenetic variation. Higher epigenetic than genetic diversities were estimated. Our outcomes showed up to 1.86 times more representation of mCG subepiloci than mCCG in all organs sampled. Along the maturing stages of floral buds, the frequency of mCG evidenced an incremental trend, whereas mCCG and unmethylated conditions showed opposite tendencies. Reproductive and vegetative samples tended to cluster apart based on epigenetic patterns; at gene expression level, organs exhibited clear-cut distinctive patterns, nonetheless profiles of young leaves and floral primordia resemble. Epigenetic and expression data allowed discrimination of I. dumosa´s samples according to the gender of the donor; more elusive patterns were observed for I. paraguariensis. In total, 102 differentially methylated and expressed fragments were characterized bioinformatically. Forty-three were annotated in various functional categories; four candidate markers were validated through qPCR, finding statistical differences among organs but not among sexes. The methylation condition of epilocus C13m33 appears as indicative of gender in both species. Thirty-three organ-specific and 34 gender-specific methylated markers were discriminated and deserve further research, particularly those expressed in leaves. Our study contributes concrete candidate markers with potential for practical application. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9008 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA90082021-03-31T13:12:30Z Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species Cascales, Jimena Acevedo, Raúl Maximiliano Paiva, Daniela Ivana Gottlieb, Alexandra Marina Ilex Ilex paraguariensis Expresión Génica Marcadores Genéticos Análisis Multivariante Metilación Gene Expression Genetic Markers Multivariate Analysis Methylation Marcadores Moleculares Yerba Mate Differential epigenetic (DNA cytosine methylation) and gene expression patterns were investigated in reproductive and vegetative organs from Ilex paraguariensis and I. dumosa, at distinct developmental stages. We aimed at contributing towards elucidating major molecular changes underlying the sexual differentiation processes which, in these dioecious species, are completely unknown. Simultaneously, as a first step towards the development of an early sexing system, we searched for promising molecular markers. This was assessed through Methylation Sensitive Amplified Polymorphism (MSAP) and Amplified Fragment Length Polymorphism on cDNA (cDNA-AFLP) techniques, applying discriminant multivariate analyses, and bioinformatic characterization of differential fragments. A significant positive correlation was found between epigenetic and indirect ‘genetic’ information for both species, indicating influence of the genetic background on the epigenetic variation. Higher epigenetic than genetic diversities were estimated. Our outcomes showed up to 1.86 times more representation of mCG subepiloci than mCCG in all organs sampled. Along the maturing stages of floral buds, the frequency of mCG evidenced an incremental trend, whereas mCCG and unmethylated conditions showed opposite tendencies. Reproductive and vegetative samples tended to cluster apart based on epigenetic patterns; at gene expression level, organs exhibited clear-cut distinctive patterns, nonetheless profiles of young leaves and floral primordia resemble. Epigenetic and expression data allowed discrimination of I. dumosa´s samples according to the gender of the donor; more elusive patterns were observed for I. paraguariensis. In total, 102 differentially methylated and expressed fragments were characterized bioinformatically. Forty-three were annotated in various functional categories; four candidate markers were validated through qPCR, finding statistical differences among organs but not among sexes. The methylation condition of epilocus C13m33 appears as indicative of gender in both species. Thirty-three organ-specific and 34 gender-specific methylated markers were discriminated and deserve further research, particularly those expressed in leaves. Our study contributes concrete candidate markers with potential for practical application. EEA Montecarlo Fil: Cascales, Jimena. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Cascales, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Acevedo, Raúl M. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste. Laboratorio de Biotecnología Aplicada y Genómica Funcional; Argentina Fil: Acevedo, Raúl M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste; Argentina Fil: Paiva, Daniela Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Paiva, Daniela Ivana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gottlieb, Alexandra Marina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Gottlieb, Alexandra Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-03-31T13:06:46Z 2021-03-31T13:06:46Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9008 https://link.springer.com/article/10.1007/s10265-021-01279-3 0918-9440 1618-0860 https://doi.org/10.1007/s10265-021-01279-3 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Journal of Plant Research ( Published: 23 March 2021) |
| spellingShingle | Ilex Ilex paraguariensis Expresión Génica Marcadores Genéticos Análisis Multivariante Metilación Gene Expression Genetic Markers Multivariate Analysis Methylation Marcadores Moleculares Yerba Mate Cascales, Jimena Acevedo, Raúl Maximiliano Paiva, Daniela Ivana Gottlieb, Alexandra Marina Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title | Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title_full | Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title_fullStr | Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title_full_unstemmed | Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title_short | Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species |
| title_sort | differential dna methylation and gene expression during development of reproductive and vegetative organs in ilex species |
| topic | Ilex Ilex paraguariensis Expresión Génica Marcadores Genéticos Análisis Multivariante Metilación Gene Expression Genetic Markers Multivariate Analysis Methylation Marcadores Moleculares Yerba Mate |
| url | http://hdl.handle.net/20.500.12123/9008 https://link.springer.com/article/10.1007/s10265-021-01279-3 https://doi.org/10.1007/s10265-021-01279-3 |
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