Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity
Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys vertic...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Elsevier
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8866 https://www.sciencedirect.com/science/article/pii/S2405844021000013 https://doi.org/10.1016/j.heliyon.2021.e05896 |
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| author | Cecchini, María Eugenia Paoloni, C. Campra, Noelia Picco, Natalia Grosso, M. Carolina Soriano Perez, María Laura Alustiza, Fabrisio Eduardo Cariddi, Laura Noelia Bellingeri, Romina |
| author_browse | Alustiza, Fabrisio Eduardo Bellingeri, Romina Campra, Noelia Cariddi, Laura Noelia Cecchini, María Eugenia Grosso, M. Carolina Paoloni, C. Picco, Natalia Soriano Perez, María Laura |
| author_facet | Cecchini, María Eugenia Paoloni, C. Campra, Noelia Picco, Natalia Grosso, M. Carolina Soriano Perez, María Laura Alustiza, Fabrisio Eduardo Cariddi, Laura Noelia Bellingeri, Romina |
| author_sort | Cecchini, María Eugenia |
| collection | INTA Digital |
| description | Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys verticillata (Griseb.) Epling (Lamiaceae) has demonstrated a strong antimicrobial activity. However, its instability and hydrophobicity under normal storage conditions are limitations to its use. Nanoemulsion technology is an excellent way to solubilize, microencapsulate, and protect this compound. This study aimed to obtain a nanoemulsion based on M. verticillata EO and evaluate its antibacterial activity against Staphylococcus aureus. The EO was obtained by steam distillation. Identification and quantification of their components were determined by GC-MS revealing that the dominated chemical group was oxygenated monoterpenes. Nanoemulsions (NE) were characterized by measuring pH, transmittance, separation percentage, release profile, and morphology. The effect of NE on the growth of S. aureus and cyto-compatibility was also evaluated. The results showed that NE containing a higher percentage of tween 20 exhibited higher stability with an approximated droplet size of 10 nm. The effect of encapsulation process was evaluated by GC-MS revealing that the volatile components in EO were no affected. After 24 h, 74.24 ± 0.75% of EO was released from NE and the antibacterial activity of EO was enhanced considerably by its encapsulation. The incubation of S. aureus with the NE and pure EO, show a bacterial growth inhibition of 58.87% ± 0.99 and 46.72% ± 3.32 (p < 0.05), respectively. In addition, nanoemulsión did not cause toxicity to porcine and equine red blood cells. The results obtained showed that NE could be a potential vehicle for M. verticillata EO with promissory properties to emerge as a tool for developing advanced therapies to control and combat infections. |
| format | Artículo |
| id | INTA8866 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA88662022-04-19T12:35:57Z Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity Cecchini, María Eugenia Paoloni, C. Campra, Noelia Picco, Natalia Grosso, M. Carolina Soriano Perez, María Laura Alustiza, Fabrisio Eduardo Cariddi, Laura Noelia Bellingeri, Romina Minthostachys Aceites Esenciales Propiedades Antimicrobianas Nanoemulsiones Essential Oils Antimicrobial Properties Nanoemulsions Minthostachys verticillata Muna Infectious diseases constitute a problem of great importance for animal and human health, as well as the increasing bacterial resistance to antibiotics. In this context, medicinal plants emerge as an effective alternative to replace the use antibiotics. The essential oil (EO) of Minthostachys verticillata (Griseb.) Epling (Lamiaceae) has demonstrated a strong antimicrobial activity. However, its instability and hydrophobicity under normal storage conditions are limitations to its use. Nanoemulsion technology is an excellent way to solubilize, microencapsulate, and protect this compound. This study aimed to obtain a nanoemulsion based on M. verticillata EO and evaluate its antibacterial activity against Staphylococcus aureus. The EO was obtained by steam distillation. Identification and quantification of their components were determined by GC-MS revealing that the dominated chemical group was oxygenated monoterpenes. Nanoemulsions (NE) were characterized by measuring pH, transmittance, separation percentage, release profile, and morphology. The effect of NE on the growth of S. aureus and cyto-compatibility was also evaluated. The results showed that NE containing a higher percentage of tween 20 exhibited higher stability with an approximated droplet size of 10 nm. The effect of encapsulation process was evaluated by GC-MS revealing that the volatile components in EO were no affected. After 24 h, 74.24 ± 0.75% of EO was released from NE and the antibacterial activity of EO was enhanced considerably by its encapsulation. The incubation of S. aureus with the NE and pure EO, show a bacterial growth inhibition of 58.87% ± 0.99 and 46.72% ± 3.32 (p < 0.05), respectively. In addition, nanoemulsión did not cause toxicity to porcine and equine red blood cells. The results obtained showed that NE could be a potential vehicle for M. verticillata EO with promissory properties to emerge as a tool for developing advanced therapies to control and combat infections. EEA Marcos Juárez Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Paoloni, C. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Campra, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Picco, Natalia. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Grosso, M. Carolina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Soriano Perez, María Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología. Laboratorio de Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina 2021-03-11T13:16:29Z 2021-03-11T13:16:29Z 2021 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8866 https://www.sciencedirect.com/science/article/pii/S2405844021000013 2405-8440 https://doi.org/10.1016/j.heliyon.2021.e05896 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 7 (1) : e05896 (January 2021) |
| spellingShingle | Minthostachys Aceites Esenciales Propiedades Antimicrobianas Nanoemulsiones Essential Oils Antimicrobial Properties Nanoemulsions Minthostachys verticillata Muna Cecchini, María Eugenia Paoloni, C. Campra, Noelia Picco, Natalia Grosso, M. Carolina Soriano Perez, María Laura Alustiza, Fabrisio Eduardo Cariddi, Laura Noelia Bellingeri, Romina Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_full | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_fullStr | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_full_unstemmed | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_short | Nanoemulsion of Minthostachys verticillata essential oil. In-vitro evaluation of its antibacterial activity |
| title_sort | nanoemulsion of minthostachys verticillata essential oil in vitro evaluation of its antibacterial activity |
| topic | Minthostachys Aceites Esenciales Propiedades Antimicrobianas Nanoemulsiones Essential Oils Antimicrobial Properties Nanoemulsions Minthostachys verticillata Muna |
| url | http://hdl.handle.net/20.500.12123/8866 https://www.sciencedirect.com/science/article/pii/S2405844021000013 https://doi.org/10.1016/j.heliyon.2021.e05896 |
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