Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8837 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13341 https://doi.org/10.1111/ppa.13341 |
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| author | Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura |
| author_browse | Bertalmío, Ana García, María Laura Godoy Suarez, Laura Magdalena Gomez, Claudio Andres Hauteville, Claudia Ruiz, Esperanza Simeone, Melina Tito, Blas Alfredo |
| author_facet | Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura |
| author_sort | Simeone, Melina |
| collection | INTA Digital |
| description | Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8837 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA88372021-03-08T16:30:41Z Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees. EEA Concordia Fil: Simeone, M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Gomez, Claudio Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Bertalmío, Ana. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental Salto Grande; Uruguay Fil: Ruiz, Esperanza. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Laboratorio de Investigación y Desarrollo de Bioactivos; Argentina Fil: Hauteville, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Godoy Suarez, Laura Magdalena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Tito, Blas Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concordia; Argentina Fil: Garcia, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina 2021-03-08T16:24:01Z 2021-03-08T16:24:01Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8837 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13341 1365-3059 https://doi.org/10.1111/ppa.13341 eng info:eu-repograntAgreement/INTA/ERIOS-630081/AR./Incremento en la calidad, cantidad y diversidad de la producción frutícola de Entre Ríos info:eu-repograntAgreement/INTA/2019-PD-E4-I081-001/2019-PD-E4-I081-001/AR./Generación de reactivos, desarrollo de metodologías, validación y acreditación de ensayos para el diagnóstico de patógenos vegetales. info:eu-repograntAgreement/INTA/2019-RIST-E4-I091-001/2019-RIST-E4-I091-001/AR./Multiplicación de propágulos de sanidad controlada info:eu-repo/semantics/restrictedAccess application/pdf Wiley Plant Pathology (First published: 18 January 2021) |
| spellingShingle | Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus Simeone, Melina Gomez, Claudio Andres Bertalmío, Ana Ruiz, Esperanza Hauteville, Claudia Godoy Suarez, Laura Magdalena Tito, Blas Alfredo García, María Laura Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title | Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_full | Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_fullStr | Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_full_unstemmed | Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_short | Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters |
| title_sort | detection of citrus psorosis virus by rt qpcr validated by diagnostic parameters |
| topic | Citrus Enfermedades de las Plantas Diagnóstico PCR Virus de las Plantas Plant Diseases Diagnosis Plant Viruses Virus de la Psoriasis de los Cítricos Citrus Psorosis Virus |
| url | http://hdl.handle.net/20.500.12123/8837 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.13341 https://doi.org/10.1111/ppa.13341 |
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