Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection
Bovine viral diarrhea virus (BVDV) and bovine gammaherpesvirus 4 (BoHV-4) infect the uterus of cattle, being responsible for huge economic losses. Most of the pathogenesis of BoHV-4 in the bovine reproductive tract has been elucidated by conducting tests on primary cultures. Thus, it is important to...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8787 https://www.sciencedirect.com/science/article/abs/pii/S0166093421000367 https://doi.org/10.1016/j.jviromet.2021.114097 |
| _version_ | 1855484267665031168 |
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| author | Romeo, Florencia Louge Uriarte, Enrique Leopoldo Delgado, Santiago Germán González Altamiranda, Erika Pereyra, Susana Beatriz Moran, Pedro Odeon, Anselmo Carlos Perez, S. Verna, Andrea Elizabeth |
| author_browse | Delgado, Santiago Germán González Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Moran, Pedro Odeon, Anselmo Carlos Pereyra, Susana Beatriz Perez, S. Romeo, Florencia Verna, Andrea Elizabeth |
| author_facet | Romeo, Florencia Louge Uriarte, Enrique Leopoldo Delgado, Santiago Germán González Altamiranda, Erika Pereyra, Susana Beatriz Moran, Pedro Odeon, Anselmo Carlos Perez, S. Verna, Andrea Elizabeth |
| author_sort | Romeo, Florencia |
| collection | INTA Digital |
| description | Bovine viral diarrhea virus (BVDV) and bovine gammaherpesvirus 4 (BoHV-4) infect the uterus of cattle, being responsible for huge economic losses. Most of the pathogenesis of BoHV-4 in the bovine reproductive tract has been elucidated by conducting tests on primary cultures. Thus, it is important to have optimal in vitro conditions, avoiding the presence of other pathogens that can alter the results. BVDV is one of the most frequent viral contaminants of cell cultures. Considering that non-cytopathic (NCP) BVDV biotype can generate persistently infected (PI) cattle, which are the major source for virus transmission in susceptible herds, it is important to check products derived from cattle that are intended to be used in research laboratories. The aim of this work was to evaluate how the natural infection of bovine endometrial cells (BEC) with a NCP BVDV strain (BEC + BVDV) affects BoHV-4 replication. We have demonstrated a delay in BoHV-4 gene expression and a decrease in viral load in the extracellular environment in BEC + BDVD cells compared to BEC (BVDV-free) cells. These results confirm that replication of BoHV-4 in BEC primary cultures is affected by previous infection with BVDV. This finding highlights the importance of ruling out BVDV infection in bovine primary cell cultures to avoid biological interference or misinterpretation of results at the time of performing in vitro studies with BoHV-4. |
| format | Artículo |
| id | INTA8787 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA87872021-03-02T16:28:38Z Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection Romeo, Florencia Louge Uriarte, Enrique Leopoldo Delgado, Santiago Germán González Altamiranda, Erika Pereyra, Susana Beatriz Moran, Pedro Odeon, Anselmo Carlos Perez, S. Verna, Andrea Elizabeth Pestivirus de la Diarrea Bovina Herpes Virus Bovino Células Replicación Viral Expresión Génica In Vitro Bovine Diarrhoa Pestivirus Bovine Herpesvirus Cells Viral Replication Gene Expression Bovine viral diarrhea virus (BVDV) and bovine gammaherpesvirus 4 (BoHV-4) infect the uterus of cattle, being responsible for huge economic losses. Most of the pathogenesis of BoHV-4 in the bovine reproductive tract has been elucidated by conducting tests on primary cultures. Thus, it is important to have optimal in vitro conditions, avoiding the presence of other pathogens that can alter the results. BVDV is one of the most frequent viral contaminants of cell cultures. Considering that non-cytopathic (NCP) BVDV biotype can generate persistently infected (PI) cattle, which are the major source for virus transmission in susceptible herds, it is important to check products derived from cattle that are intended to be used in research laboratories. The aim of this work was to evaluate how the natural infection of bovine endometrial cells (BEC) with a NCP BVDV strain (BEC + BVDV) affects BoHV-4 replication. We have demonstrated a delay in BoHV-4 gene expression and a decrease in viral load in the extracellular environment in BEC + BDVD cells compared to BEC (BVDV-free) cells. These results confirm that replication of BoHV-4 in BEC primary cultures is affected by previous infection with BVDV. This finding highlights the importance of ruling out BVDV infection in bovine primary cell cultures to avoid biological interference or misinterpretation of results at the time of performing in vitro studies with BoHV-4. EEA Balcarce Fil: Romeo, Florencia. Agencia Nacional de Promoción Científica y Tecnológica; Argentina. Universidad Nacional de Mar del Plata, Argentina. Facultad de Ciencias Agrarias; Argentina. Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: González Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Morán, Pedro. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Odeón, Anselmo Carlos. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Pérez, S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina. Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. 2021-03-02T16:11:59Z 2021-03-02T16:11:59Z 2021-02-15 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8787 https://www.sciencedirect.com/science/article/abs/pii/S0166093421000367 0166-0934 https://doi.org/10.1016/j.jviromet.2021.114097 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Virological Methods 291 : 114097 (2021) |
| spellingShingle | Pestivirus de la Diarrea Bovina Herpes Virus Bovino Células Replicación Viral Expresión Génica In Vitro Bovine Diarrhoa Pestivirus Bovine Herpesvirus Cells Viral Replication Gene Expression Romeo, Florencia Louge Uriarte, Enrique Leopoldo Delgado, Santiago Germán González Altamiranda, Erika Pereyra, Susana Beatriz Moran, Pedro Odeon, Anselmo Carlos Perez, S. Verna, Andrea Elizabeth Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title | Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title_full | Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title_fullStr | Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title_full_unstemmed | Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title_short | Effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture: An in vitro model of viral co-infection |
| title_sort | effect of bovine viral diarrhea virus on subsequent infectivity of bovine gammaherpesvirus 4 in endometrial cells in primary culture an in vitro model of viral co infection |
| topic | Pestivirus de la Diarrea Bovina Herpes Virus Bovino Células Replicación Viral Expresión Génica In Vitro Bovine Diarrhoa Pestivirus Bovine Herpesvirus Cells Viral Replication Gene Expression |
| url | http://hdl.handle.net/20.500.12123/8787 https://www.sciencedirect.com/science/article/abs/pii/S0166093421000367 https://doi.org/10.1016/j.jviromet.2021.114097 |
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