Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus s...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8779 https://www.sciencedirect.com/science/article/pii/S2451943X20300764 https://doi.org/10.1016/j.vas.2020.100163 |
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| author | Delpiazzo, Rafael Barcellos, Maila Barros, Sofia Bentacor, Laura Fraga, Martin Gil, Jorge Iraola, Gregorio Morsella, Claudia Graciela Paolicchi, Fernando Perez, Ruben Riet Correa, Franklin Sanguinetti, Margarita Silva Silveira, Caroline da Caballeros, Lucía |
| author_browse | Barcellos, Maila Barros, Sofia Bentacor, Laura Caballeros, Lucía Delpiazzo, Rafael Fraga, Martin Gil, Jorge Iraola, Gregorio Morsella, Claudia Graciela Paolicchi, Fernando Perez, Ruben Riet Correa, Franklin Sanguinetti, Margarita Silva Silveira, Caroline da |
| author_facet | Delpiazzo, Rafael Barcellos, Maila Barros, Sofia Bentacor, Laura Fraga, Martin Gil, Jorge Iraola, Gregorio Morsella, Claudia Graciela Paolicchi, Fernando Perez, Ruben Riet Correa, Franklin Sanguinetti, Margarita Silva Silveira, Caroline da Caballeros, Lucía |
| author_sort | Delpiazzo, Rafael |
| collection | INTA Digital |
| description | Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
| format | Artículo |
| id | INTA8779 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA87792021-03-02T11:28:38Z Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence Delpiazzo, Rafael Barcellos, Maila Barros, Sofia Bentacor, Laura Fraga, Martin Gil, Jorge Iraola, Gregorio Morsella, Claudia Graciela Paolicchi, Fernando Perez, Ruben Riet Correa, Franklin Sanguinetti, Margarita Silva Silveira, Caroline da Caballeros, Lucía Ganado Bovino Toro Diagnóstico PCR Campylobacter Fetus Campilobacteriosis Cattle Bulls Diagnosis Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. EEA Balcarce Fil: Delpiazzo, Rafael. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay. Fil: Barcellos, Maila. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. Fil: Barros, Sofía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. Fil: Bentacor, Laura. Universidad de la República. Facultad de Medicina; Uruguay. Fil: Fraga, Martín. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay. Fil: Gil, Jorge. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay. Fil: Iraola, Gregorio. Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana; Uruguay. Universidad Mayor. Facultad de Ciencias; Chile. Wellcome Genome Campus, Wellcome Sanger Institute; United Kingdom. Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Pérez, Ruben. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. Fil: Riet-Correa, Franklin. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay. Fil: Sanguinetti, Margarita. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. Fil: Silva, Alfonso. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. Fil: Silva Silveira, Caroline da. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay. Fil: Caballeros, Lucía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay. 2021-03-02T11:23:34Z 2021-03-02T11:23:34Z 2020-12-24 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8779 https://www.sciencedirect.com/science/article/pii/S2451943X20300764 2451-943X https://doi.org/10.1016/j.vas.2020.100163 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Veterinary and Animal Science 11 : 100163 (March 2021) |
| spellingShingle | Ganado Bovino Toro Diagnóstico PCR Campylobacter Fetus Campilobacteriosis Cattle Bulls Diagnosis Delpiazzo, Rafael Barcellos, Maila Barros, Sofia Bentacor, Laura Fraga, Martin Gil, Jorge Iraola, Gregorio Morsella, Claudia Graciela Paolicchi, Fernando Perez, Ruben Riet Correa, Franklin Sanguinetti, Margarita Silva Silveira, Caroline da Caballeros, Lucía Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_full | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_fullStr | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_full_unstemmed | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_short | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_sort | accurate and fast identification of campylobacter fetus in bulls by real time pcr targeting a 16s rrna gene sequence |
| topic | Ganado Bovino Toro Diagnóstico PCR Campylobacter Fetus Campilobacteriosis Cattle Bulls Diagnosis |
| url | http://hdl.handle.net/20.500.12123/8779 https://www.sciencedirect.com/science/article/pii/S2451943X20300764 https://doi.org/10.1016/j.vas.2020.100163 |
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