Spiraea sp. new natural host of Plum pox virus (Sharka)
Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8777 https://link.springer.com/article/10.1007/s10658-021-02206-x https://doi.org/10.1007/s10658-021-02206-x |
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| author | Piglionico, Damián Gustavo Ojeda, Eva Maria Lucero, Vanesa Angelica Farrando, Roberto Jes Porcel, Laura Beatriz Picca, Cecilia Nelida Marini, Diana Beatriz |
| author_browse | Farrando, Roberto Jes Lucero, Vanesa Angelica Marini, Diana Beatriz Ojeda, Eva Maria Picca, Cecilia Nelida Piglionico, Damián Gustavo Porcel, Laura Beatriz |
| author_facet | Piglionico, Damián Gustavo Ojeda, Eva Maria Lucero, Vanesa Angelica Farrando, Roberto Jes Porcel, Laura Beatriz Picca, Cecilia Nelida Marini, Diana Beatriz |
| author_sort | Piglionico, Damián Gustavo |
| collection | INTA Digital |
| description | Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8777 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA87772021-03-01T18:08:15Z Spiraea sp. new natural host of Plum pox virus (Sharka) Piglionico, Damián Gustavo Ojeda, Eva Maria Lucero, Vanesa Angelica Farrando, Roberto Jes Porcel, Laura Beatriz Picca, Cecilia Nelida Marini, Diana Beatriz Virus Sharka del Ciruelo Virus de las Plantas Spiraea Huéspedes Identificación Plum Pox Virus Plant Viruses Hosts Identification Sharka Plum pox virus (PPV) was detected in Argentina for the first time in 2004. After eradication of the initial outbreaks, establishment of quarantine areas and strict control of nursery stock plants, no evidence of disease spread was recorded. During spring 2018, symptoms, consisting of chlorotic rings, were observed on leaves of an ornamental plant of the Rosaceae family “Bridal Crown” (Spiraea sp.) in the garden of a house within the PPV quarantine area in San Rafael, Mendoza province. Symptomatic leaves were analyzed for PPV by DAS-ELISA with positive results. The presence of the virus was confirmed by conventional and qRT-PCR and the use of “GF-305” biological indicator plants inoculated by T-bud grafting with buds from the Spiraea. A 243 bp PCR fragment amplified from the Spiraea samples, and corresponding to the carboxy-terminal region of PPV coat protein gene was isolated, sequenced, and deposited in GenBank (MN587037). It showed 99% identity to other PPV isolates. RT-qPCR, confirmed the virus as being an isolate of the PPV D strain previously found in the quarantine areas. Our results confirm PPV natural infection on a new ornamental host, “Bridal Crown”, which does not belong to the genus Prunus, warning of the potential danger of the species as a host of, and a source of, inoculum for PPV. EEA Junín Fil: Piglionico, Damián Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina Fil: Ojeda, Eva Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Lucero, Vanesa Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina Fil: Farrando, Roberto Jes. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina Fil: Porcel, Laura Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Picca, Cecilia Nelida. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rama Caída; Argentina Fil: Marini, Diana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Junín; Argentina 2021-03-01T18:03:51Z 2021-03-01T18:03:51Z 2021-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8777 https://link.springer.com/article/10.1007/s10658-021-02206-x 0929-1873 1573-8469 https://doi.org/10.1007/s10658-021-02206-x eng info:eu-repo/semantics/restrictedAccess application/pdf Springer European Journal of Plant Pathology (Published: 27 January 2021) |
| spellingShingle | Virus Sharka del Ciruelo Virus de las Plantas Spiraea Huéspedes Identificación Plum Pox Virus Plant Viruses Hosts Identification Sharka Piglionico, Damián Gustavo Ojeda, Eva Maria Lucero, Vanesa Angelica Farrando, Roberto Jes Porcel, Laura Beatriz Picca, Cecilia Nelida Marini, Diana Beatriz Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title | Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title_full | Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title_fullStr | Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title_full_unstemmed | Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title_short | Spiraea sp. new natural host of Plum pox virus (Sharka) |
| title_sort | spiraea sp new natural host of plum pox virus sharka |
| topic | Virus Sharka del Ciruelo Virus de las Plantas Spiraea Huéspedes Identificación Plum Pox Virus Plant Viruses Hosts Identification Sharka |
| url | http://hdl.handle.net/20.500.12123/8777 https://link.springer.com/article/10.1007/s10658-021-02206-x https://doi.org/10.1007/s10658-021-02206-x |
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