Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles
Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends,...
| Autores principales: | , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer Nature
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8762 https://link.springer.com/article/10.1007/s10722-020-01034-y https://doi.org/10.1007/s10722-020-01034-y |
| _version_ | 1855484262844727296 |
|---|---|
| author | Poulsen Hornum, Anabela Camadro, Elsa Lucila |
| author_browse | Camadro, Elsa Lucila Poulsen Hornum, Anabela |
| author_facet | Poulsen Hornum, Anabela Camadro, Elsa Lucila |
| author_sort | Poulsen Hornum, Anabela |
| collection | INTA Digital |
| description | Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises. |
| format | Artículo |
| id | INTA8762 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | INTA87622021-02-26T13:24:55Z Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles Poulsen Hornum, Anabela Camadro, Elsa Lucila Papa Potatoes Solanum Chacoense Banco de Germoplasma Germplasm Banks Varieties Variedades Papa Silvestre Wild potatoes (Solanum sp.) are important reservoirs of desirable genes for potato breeding. Most of them are diploid and obligate outcrossers, with both sexual and asexual reproduction. Samples of natural populations are ex situ conserved as accessions in germplasm banks for basic and applied ends, and are regenerated when seed number, seed viability or both decrease. The possible action of within accession internal reproductive barriers is not considered in the current seed regeneration protocols, in which 20 to 25 plants are cultivated for the crossing work. Notwithstanding, the effective number of progenitors (Ne) in every seed regeneration cycle might be reduced in relation to the real number (N) of plants if internal reproductive barriers were acting; this process can eventually lead to gene erosion. To ascertain if Ne = N in seed regeneration of one randomly provided accession of S. chacoense Bitter, pollen viability and size, plus pollen-pistil relationships and number and type (according to endosperm development) of seeds/fruit were studied, respectively, at the individual plant and genotypic combination levels. Two types of barriers, pre-zygotic (pollen-pistil self- and cross-incompatibility) and post-zygotic (male sterility and inviability and weakness of F1 hybrids) were detected in three growing seasons, indicating that not all genotypes in this accession made an equal allele contribution to the next generation. These results should be an alert for germplasm bank curators of wild potatoes and other crop wild relatives. Since the objective of germplasm banks is the ex situ maintenance of the natural genetic diversity, strategies need to be devised based on genetic premises. EEA Balcarce Fil: Poulsen Hornum, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Camadro, Elsa Lucila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-02-26T13:14:42Z 2021-02-26T13:14:42Z 2020-10-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8762 https://link.springer.com/article/10.1007/s10722-020-01034-y 1573-5109 (online) 0925-9864 (print) https://doi.org/10.1007/s10722-020-01034-y eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Nature Genetic Resources and Crop Evolution 68 : 915–938 (2021) |
| spellingShingle | Papa Potatoes Solanum Chacoense Banco de Germoplasma Germplasm Banks Varieties Variedades Papa Silvestre Poulsen Hornum, Anabela Camadro, Elsa Lucila Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title | Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title_full | Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title_fullStr | Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title_full_unstemmed | Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title_short | Expression of internal reproductive barriers in a germplasm bank accession of the wild potato Solanum chacoense Bitter in three ex situ regeneration cycles |
| title_sort | expression of internal reproductive barriers in a germplasm bank accession of the wild potato solanum chacoense bitter in three ex situ regeneration cycles |
| topic | Papa Potatoes Solanum Chacoense Banco de Germoplasma Germplasm Banks Varieties Variedades Papa Silvestre |
| url | http://hdl.handle.net/20.500.12123/8762 https://link.springer.com/article/10.1007/s10722-020-01034-y https://doi.org/10.1007/s10722-020-01034-y |
| work_keys_str_mv | AT poulsenhornumanabela expressionofinternalreproductivebarriersinagermplasmbankaccessionofthewildpotatosolanumchacoensebitterinthreeexsituregenerationcycles AT camadroelsalucila expressionofinternalreproductivebarriersinagermplasmbankaccessionofthewildpotatosolanumchacoensebitterinthreeexsituregenerationcycles |