Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum
Neospora caninum is a leading cause of abortion in cattle worldwide. The study of the immune response against N. caninum is critical to understand its epidemiology, pathogenesis, diagnosis and, ultimately, in preventing and controlling bovine neosporosis. Herein, we determined the gene expression o...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8761 https://www.sciencedirect.com/science/article/abs/pii/S0161589021000249 https://doi.org/10.1016/j.molimm.2021.01.016 |
| _version_ | 1855036094032117760 |
|---|---|
| author | Maldonado Rivera, J.E. Hecker, Yanina Burucúa, Mercedes María Cirone, Karina Mariela Cheuquepan Valenzuel, Felipe Andres Fiorani, Franco Dorsch, Matias Colque Caro, Luis Adrián Canton, German Jose Marin, Maia Solange Moore, Prando Dadin |
| author_browse | Burucúa, Mercedes María Canton, German Jose Cheuquepan Valenzuel, Felipe Andres Cirone, Karina Mariela Colque Caro, Luis Adrián Dorsch, Matias Fiorani, Franco Hecker, Yanina Maldonado Rivera, J.E. Marin, Maia Solange Moore, Prando Dadin |
| author_facet | Maldonado Rivera, J.E. Hecker, Yanina Burucúa, Mercedes María Cirone, Karina Mariela Cheuquepan Valenzuel, Felipe Andres Fiorani, Franco Dorsch, Matias Colque Caro, Luis Adrián Canton, German Jose Marin, Maia Solange Moore, Prando Dadin |
| author_sort | Maldonado Rivera, J.E. |
| collection | INTA Digital |
| description | Neospora caninum is a leading cause of abortion in cattle worldwide. The study of the immune response against
N. caninum is critical to understand its epidemiology, pathogenesis, diagnosis and, ultimately, in preventing and controlling bovine neosporosis. Herein, we determined the gene expression of innate immune components endosomal RNA-sensing TLRs, BMAP28 cathelicidin, TNF-α and IL-10 and characterized the variation in both IgG ratio and avidity at delivery in N. caninum-infected heifers challenged at day 210 of gestation, colostrum and their calves.
Increased BMAP28 expression was observed not only in colostrum but also in peripheral blood mononuclear cells (PBMC) and umbilical cord of calves from N. caninum-infected heifers in comparison with mock-infected control group. In addition, statistically significant decrease of TLR7 and IL-10 expression levels were observed in umbilical cord, suggesting an attempt to avoid an exacerbated immune response against the parasite. At
delivery, serum and colostrum samples from infected group evidenced specific IgG anti-N. caninum. Infected heifers showed IgG1/IgG2 ratios <1 and high avidity specific IgG. As expected, colostrum samples of these an- imals exhibited a high IgG1 concentration and elevated avidity values. Three out of four calves from N. caninum- infected heifers had specific IgG with IgG1/IgG2 ratios>1 and lower avidity values before colostrum intake. Interestingly, both IgG1/IgG2 ratios and avidity values increased in seropositive calves after colostrum intake.
Overall, this study provides novel information on neonatal immunity in congenitally infected calves, which is essential to understand how the immune pathways could be manipulated or immune components could be employed in order to improve protection against neosporosis. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8761 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA87612021-02-26T13:04:34Z Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum Maldonado Rivera, J.E. Hecker, Yanina Burucúa, Mercedes María Cirone, Karina Mariela Cheuquepan Valenzuel, Felipe Andres Fiorani, Franco Dorsch, Matias Colque Caro, Luis Adrián Canton, German Jose Marin, Maia Solange Moore, Prando Dadin Neospora Caninum Vaca Respuesta Inmunológica Calostro Enfermedades de los Animales Cows Immune Response Colostrum Animals Diseases Neospora caninum is a leading cause of abortion in cattle worldwide. The study of the immune response against N. caninum is critical to understand its epidemiology, pathogenesis, diagnosis and, ultimately, in preventing and controlling bovine neosporosis. Herein, we determined the gene expression of innate immune components endosomal RNA-sensing TLRs, BMAP28 cathelicidin, TNF-α and IL-10 and characterized the variation in both IgG ratio and avidity at delivery in N. caninum-infected heifers challenged at day 210 of gestation, colostrum and their calves. Increased BMAP28 expression was observed not only in colostrum but also in peripheral blood mononuclear cells (PBMC) and umbilical cord of calves from N. caninum-infected heifers in comparison with mock-infected control group. In addition, statistically significant decrease of TLR7 and IL-10 expression levels were observed in umbilical cord, suggesting an attempt to avoid an exacerbated immune response against the parasite. At delivery, serum and colostrum samples from infected group evidenced specific IgG anti-N. caninum. Infected heifers showed IgG1/IgG2 ratios <1 and high avidity specific IgG. As expected, colostrum samples of these an- imals exhibited a high IgG1 concentration and elevated avidity values. Three out of four calves from N. caninum- infected heifers had specific IgG with IgG1/IgG2 ratios>1 and lower avidity values before colostrum intake. Interestingly, both IgG1/IgG2 ratios and avidity values increased in seropositive calves after colostrum intake. Overall, this study provides novel information on neonatal immunity in congenitally infected calves, which is essential to understand how the immune pathways could be manipulated or immune components could be employed in order to improve protection against neosporosis. EEA Balcarce Fil: Maldonado Rivera, J.E. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Universidad de Cuenca. Facultad de Ciencias Agropecuarias; Ecuador. Fil: Hecker, Yanina Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina. Fil: Burucúa, Mercedes María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina. Fil: Cirone, Karina Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: Fiorani, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Dorsch, Matías. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Fil: Colque Caro, Luis Adrián. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina. Fil: Moore, Dadín Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina. 2021-02-26T12:55:24Z 2021-02-26T12:55:24Z 2021-02-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8761 https://www.sciencedirect.com/science/article/abs/pii/S0161589021000249 0161-5890 https://doi.org/10.1016/j.molimm.2021.01.016 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Molecular Immunology 132 : 53-59 (2021) |
| spellingShingle | Neospora Caninum Vaca Respuesta Inmunológica Calostro Enfermedades de los Animales Cows Immune Response Colostrum Animals Diseases Maldonado Rivera, J.E. Hecker, Yanina Burucúa, Mercedes María Cirone, Karina Mariela Cheuquepan Valenzuel, Felipe Andres Fiorani, Franco Dorsch, Matias Colque Caro, Luis Adrián Canton, German Jose Marin, Maia Solange Moore, Prando Dadin Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title | Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title_full | Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title_fullStr | Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title_full_unstemmed | Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title_short | Innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with Neospora caninum |
| title_sort | innate and humoral immune parameters at delivery in colostrum and calves from heifers experimentally infected with neospora caninum |
| topic | Neospora Caninum Vaca Respuesta Inmunológica Calostro Enfermedades de los Animales Cows Immune Response Colostrum Animals Diseases |
| url | http://hdl.handle.net/20.500.12123/8761 https://www.sciencedirect.com/science/article/abs/pii/S0161589021000249 https://doi.org/10.1016/j.molimm.2021.01.016 |
| work_keys_str_mv | AT maldonadoriveraje innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT heckeryanina innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT burucuamercedesmaria innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT cironekarinamariela innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT cheuquepanvalenzuelfelipeandres innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT fioranifranco innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT dorschmatias innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT colquecaroluisadrian innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT cantongermanjose innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT marinmaiasolange innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum AT mooreprandodadin innateandhumoralimmuneparametersatdeliveryincolostrumandcalvesfromheifersexperimentallyinfectedwithneosporacaninum |