Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island
Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Oxford University Press
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8727 https://academic.oup.com/femsle/article/362/22/fnv189/1816015 https://doi.org/10.1093/femsle/fnv189 |
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| author | Iribarren, Maria Josefina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_browse | Ayub, Nicolás Daniel Iribarren, Maria Josefina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia |
| author_facet | Iribarren, Maria Josefina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia Ayub, Nicolás Daniel |
| author_sort | Iribarren, Maria Josefina |
| collection | INTA Digital |
| description | Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora. |
| format | Artículo |
| id | INTA8727 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | INTA87272021-02-23T13:34:50Z Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island Iribarren, Maria Josefina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia Ayub, Nicolás Daniel Phytophthora capsici Hortalizas (plantas) Organismos Patógenos Efectores Moleculares Genómica Vegetable Crops Pathogens Effectors Genomics Phytophthora capsici is a virulent oomycete pathogen of many vegetable crops. Recently, it has been demonstrated that the recognition of the RXLR effector AVR3a1 of P. capsici (PcAVR3a1) triggers a hypersensitive response and plays a critical role in mediating non-host resistance. Here, we analyzed the occurrence of PcAVR3a1 in 57 isolates of P. capsici derived from globe squash, eggplant, tomato and bell pepper cocultivated in a small geographical area. The occurrence of PcAVR3a1 in environmental strains of P. capsici was confirmed by PCR in only 21 of these pathogen isolates. To understand the presence-absence pattern of PcAVR3a1 in environmental strains, the flanking region of this gene was sequenced. PcAVR3a1 was found within a genetic element that we named PcAVR3a1-GI (PcAVR3a1 genomic island). PcAVR3a1-GI was flanked by a 22-bp direct repeat, which is related to its site-specific recombination site. In addition to the PcAVR3a1 gene, PcAVR3a1-GI also encoded a phage integrase probably associated with the excision and integration of this mobile element. Exposure to plant induced the presence of an episomal circular intermediate of PcAVR3a1-GI, indicating that this mobile element is functional. Collectively, these findings provide evidence of PcAVR3a1 evolution via mobile elements in environmental strains of Phytophthora. Instituto de Genética Fil: Iribarren, Maria Josefina. Universidad Nacional de Luján; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pascuan, Cecilia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-02-23T13:25:34Z 2021-02-23T13:25:34Z 2015-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8727 https://academic.oup.com/femsle/article/362/22/fnv189/1816015 1574-6968 https://doi.org/10.1093/femsle/fnv189 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford University Press FEMS Microbiology Letters 362 (22) : fnv189 (Octubre 2015) |
| spellingShingle | Phytophthora capsici Hortalizas (plantas) Organismos Patógenos Efectores Moleculares Genómica Vegetable Crops Pathogens Effectors Genomics Iribarren, Maria Josefina Pascuan, Cecilia Gabriela Soto, Gabriela Cynthia Ayub, Nicolás Daniel Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title | Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title_full | Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title_fullStr | Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title_full_unstemmed | Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title_short | Genetic analysis of environmental strains of the plant pathogen Phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| title_sort | genetic analysis of environmental strains of the plant pathogen phytophthora capsici reveals heterogeneous repertoire of effectors and possible effector evolution via genomic island |
| topic | Phytophthora capsici Hortalizas (plantas) Organismos Patógenos Efectores Moleculares Genómica Vegetable Crops Pathogens Effectors Genomics |
| url | http://hdl.handle.net/20.500.12123/8727 https://academic.oup.com/femsle/article/362/22/fnv189/1816015 https://doi.org/10.1093/femsle/fnv189 |
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