Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent
Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic ana...
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| Format: | Artículo |
| Language: | Inglés |
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Czech Academy of Sciences
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8725 https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php https://doi.org/10.14411/eje.2013.054 |
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| author | Carabajal Paladino, Leonale Suzel Papeschi, Alba Graciela Lanzavecchia, Silvia Beatriz Cladera, Jorge Luis Bressa, María José |
| author_browse | Bressa, María José Carabajal Paladino, Leonale Suzel Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Papeschi, Alba Graciela |
| author_facet | Carabajal Paladino, Leonale Suzel Papeschi, Alba Graciela Lanzavecchia, Silvia Beatriz Cladera, Jorge Luis Bressa, María José |
| author_sort | Carabajal Paladino, Leonale Suzel |
| collection | INTA Digital |
| description | Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies. |
| format | Artículo |
| id | INTA8725 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Czech Academy of Sciences |
| publisherStr | Czech Academy of Sciences |
| record_format | dspace |
| spelling | INTA87252021-02-23T13:05:47Z Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent Carabajal Paladino, Leonale Suzel Papeschi, Alba Graciela Lanzavecchia, Silvia Beatriz Cladera, Jorge Luis Bressa, María José Hymenoptera Braconidae Biosteres longicaudatus Cariotipos Heterocromatina ADN ribosomal Citogenética Parasitoides Agentes de Control Biológico Karyotypes Heterochromatin Ribosomal DNA Cytogenetics Parasitoids Biological Control Agents Diachasmimorpha longicaudata Diachasmimorpha longicaudata (Hymenoptera: Braconidae) is a parasitoid wasp widely used in the biological control of fruit flies. In this paper, we present a detailed analysis of the karyotype of this species based on the results of classical and molecular cytogenetic techniques. The cytogenetic analysis confirmed the male and female chromosome numbers previously reported (n = 20, 2n = 40). The entire short arm of most chromosomes is made up of a large constitutive heterochromatic segment. The high heterochromatin content differentiates D. longicaudata from other braconid species. Fluorescence in situ hybridization (FISH) using autologous 18S rDNA probes revealed six clusters of rDNA, i.e. six nucleolar organizer regions (NORs), in the heterochromatic short arms of different chromosomes in the haploid male karyotype. This number is exceptionally high for Hymenoptera, which usually have two NORs in the diploid complement. It is noteworthy that these rDNA-FISH experiments represent the first use of this technique on a braconid species using autologous probes. Since Ag-NOR-bands were coincident with C-positive bands on metaphase chromosomes, it was not possible to identify active nucleoli. The physical characteristics of the D. longicaudata karyotype, especially the content and distribution of heterochromatin and the number and location of rDNA clusters, contribute to a better understanding of the structure and organization of braconid chromosomes and provide a basis for genomic and evolutionary studies. Instituto de Genética Fil: Carabajal Paladino, Leonela Zusel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Papeschi, Alba Graciela. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina Fil: Bressa, Maria Jose. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Genética y Evolución. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina 2021-02-23T12:59:01Z 2021-02-23T12:59:01Z 2013-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8725 https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php 1802-8829 https://doi.org/10.14411/eje.2013.054 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Czech Academy of Sciences European Journal of Entomology 110 (3) : 401-409 (Julio 2013) |
| spellingShingle | Hymenoptera Braconidae Biosteres longicaudatus Cariotipos Heterocromatina ADN ribosomal Citogenética Parasitoides Agentes de Control Biológico Karyotypes Heterochromatin Ribosomal DNA Cytogenetics Parasitoids Biological Control Agents Diachasmimorpha longicaudata Carabajal Paladino, Leonale Suzel Papeschi, Alba Graciela Lanzavecchia, Silvia Beatriz Cladera, Jorge Luis Bressa, María José Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title | Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title_full | Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title_fullStr | Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title_full_unstemmed | Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title_short | Cytogenetic characterization of Diachasmimorpha longicaudata (Hymenoptera: Braconidae), a parasitoid wasp used as a biological control agent |
| title_sort | cytogenetic characterization of diachasmimorpha longicaudata hymenoptera braconidae a parasitoid wasp used as a biological control agent |
| topic | Hymenoptera Braconidae Biosteres longicaudatus Cariotipos Heterocromatina ADN ribosomal Citogenética Parasitoides Agentes de Control Biológico Karyotypes Heterochromatin Ribosomal DNA Cytogenetics Parasitoids Biological Control Agents Diachasmimorpha longicaudata |
| url | http://hdl.handle.net/20.500.12123/8725 https://www.eje.cz/artkey/eje-201303-0003_Cytogenetic_characterization_of_Diachasmimorpha_longicaudata_Hymenoptera_Braconidae_a_parasitoid_wasp_used.php https://doi.org/10.14411/eje.2013.054 |
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