Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. = Transferencia cruzada de marcadores SSR en Setaria sphacelata y Trichloris crinita sp.
Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We perf...
| Main Authors: | , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Universidad Nacional de Colombia
2021
|
| Subjects: | |
| Online Access: | https://revistas.unal.edu.co/index.php/agrocol/article/view/78785 http://hdl.handle.net/20.500.12123/8708 https://doi.org/10.15446/agron.colomb.v37n2.78785 |
| Summary: | Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species. |
|---|