Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization
Microbial communities associated to insect species are involved in essential biological functions such as host nutrition, reproduction and survivability. Main factors have been described as modulators of gut bacterial community, such as diet, habit, developmental stage and taxonomy of the host. The...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Frontiers Media
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/8706 https://www.frontiersin.org/articles/10.3389/fmicb.2020.570960/full https://doi.org/10.3389/fmicb.2020.570960 |
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| author | Salgueiro, Julieta Pimper, Lidia Elena Segura, Diego Fernando Milla, Fabian Horacio Russo, Romina Maria Asimakis, Elias D. Stathopoulou, Panagiota Bourtzis, Kostas Cladera, Jorge Luis Tsiamis, George Lanzavecchia, Silvia Beatriz |
| author_browse | Asimakis, Elias D. Bourtzis, Kostas Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Milla, Fabian Horacio Pimper, Lidia Elena Russo, Romina Maria Salgueiro, Julieta Segura, Diego Fernando Stathopoulou, Panagiota Tsiamis, George |
| author_facet | Salgueiro, Julieta Pimper, Lidia Elena Segura, Diego Fernando Milla, Fabian Horacio Russo, Romina Maria Asimakis, Elias D. Stathopoulou, Panagiota Bourtzis, Kostas Cladera, Jorge Luis Tsiamis, George Lanzavecchia, Silvia Beatriz |
| author_sort | Salgueiro, Julieta |
| collection | INTA Digital |
| description | Microbial communities associated to insect species are involved in essential biological functions such as host nutrition, reproduction and survivability. Main factors have been described as modulators of gut bacterial community, such as diet, habit, developmental stage and taxonomy of the host. The present work focuses on the complex changes that gut microbial communities go through when wild insects are introduced to artificial rearing conditions. Specifically, we analyzed the effect of the laboratory colonization on the richness and diversity of the gut bacteriome hosted by the fruit fly pest Anastrepha fraterculus sp. 1. Bacterial profiles were studied by amplicon sequencing of the 16S rRNA V3–V4 hypervariable region in gut samples of males and females, in teneral (1-day-old, unfed) and post-teneral (15-day-old, fed) flies. A total of 3,147,665 sequence reads were obtained and 32 bacterial operational taxonomic units (OTUs) were identified. Proteobacteria was the most abundant phylum (93.3% of the total reads) and, Wolbachia and Enterobacter were the most represented taxa at the genus level (29.9% and 27.7%, respectively, of the total read counts). Wild and laboratory flies showed highly significant differences in the relative abundances of bacteria. The analysis of the core bacteriome showed the presence of five OTUs in all samples grouped by origin, while nine and five OTUs were exclusively detected in laboratory and wild flies, respectively. Irrespective of fly origin or sex, a dominant presence of Wolbachia was observed in teneral flies, whereas Enterobacter was highly abundant in post-teneral individuals. We evidenced significant differences in bacterial richness and diversity among generations under laboratory colonization (F0, F1, F3 and F6) and compared to laboratory and wild flies, displaying also differential patterns between teneral and post-teneral flies. Laboratory and wild A. fraterculus sp. 1 harbor different gut bacterial communities. Laboratory colonization has an important effect on the microbiota, most likely associated to the combined effects of insect physiology and environmental conditions (e.g., diet and colony management). |
| format | Artículo |
| id | INTA8706 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA87062021-02-22T11:40:57Z Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization Salgueiro, Julieta Pimper, Lidia Elena Segura, Diego Fernando Milla, Fabian Horacio Russo, Romina Maria Asimakis, Elias D. Stathopoulou, Panagiota Bourtzis, Kostas Cladera, Jorge Luis Tsiamis, George Lanzavecchia, Silvia Beatriz Taxonomy Enterobacter Taxonomía Anastrepha fraterculus Sterile Insect Technique Bacterial Diversity Bacterial Richness Técnica del Insecto Estéril Diversidad Bacteriana Riqueza Bacteriana Microbial communities associated to insect species are involved in essential biological functions such as host nutrition, reproduction and survivability. Main factors have been described as modulators of gut bacterial community, such as diet, habit, developmental stage and taxonomy of the host. The present work focuses on the complex changes that gut microbial communities go through when wild insects are introduced to artificial rearing conditions. Specifically, we analyzed the effect of the laboratory colonization on the richness and diversity of the gut bacteriome hosted by the fruit fly pest Anastrepha fraterculus sp. 1. Bacterial profiles were studied by amplicon sequencing of the 16S rRNA V3–V4 hypervariable region in gut samples of males and females, in teneral (1-day-old, unfed) and post-teneral (15-day-old, fed) flies. A total of 3,147,665 sequence reads were obtained and 32 bacterial operational taxonomic units (OTUs) were identified. Proteobacteria was the most abundant phylum (93.3% of the total reads) and, Wolbachia and Enterobacter were the most represented taxa at the genus level (29.9% and 27.7%, respectively, of the total read counts). Wild and laboratory flies showed highly significant differences in the relative abundances of bacteria. The analysis of the core bacteriome showed the presence of five OTUs in all samples grouped by origin, while nine and five OTUs were exclusively detected in laboratory and wild flies, respectively. Irrespective of fly origin or sex, a dominant presence of Wolbachia was observed in teneral flies, whereas Enterobacter was highly abundant in post-teneral individuals. We evidenced significant differences in bacterial richness and diversity among generations under laboratory colonization (F0, F1, F3 and F6) and compared to laboratory and wild flies, displaying also differential patterns between teneral and post-teneral flies. Laboratory and wild A. fraterculus sp. 1 harbor different gut bacterial communities. Laboratory colonization has an important effect on the microbiota, most likely associated to the combined effects of insect physiology and environmental conditions (e.g., diet and colony management). Instituto de Genética Fil: Salgueiro, Julieta. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pimper, Lidia Elena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asimakis, Elias D. University of Patras. Department of Environmental Engineering; Grecia Fil: Stathopoulou, Panagiota. University of Patras. Department of Environmental Engineering; Grecia Fil: Bourtzis, Kostas. Vienna International Centre. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture. Insect Pest Control Laboratory; Austria Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tsiamis, George. University of Patras. Department of Environmental Engineering; Grecia Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret". Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-02-22T11:35:37Z 2021-02-22T11:35:37Z 2020-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8706 https://www.frontiersin.org/articles/10.3389/fmicb.2020.570960/full 1664-302X https://doi.org/10.3389/fmicb.2020.570960 eng info:eu-repograntAgreement/INTA/AEBIO-242411/AR./Epidemiología molecular agropecuaria de patógenos y plagas de importancia económica info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Microbiology 11 : 570960 (Octubre 2020) |
| spellingShingle | Taxonomy Enterobacter Taxonomía Anastrepha fraterculus Sterile Insect Technique Bacterial Diversity Bacterial Richness Técnica del Insecto Estéril Diversidad Bacteriana Riqueza Bacteriana Salgueiro, Julieta Pimper, Lidia Elena Segura, Diego Fernando Milla, Fabian Horacio Russo, Romina Maria Asimakis, Elias D. Stathopoulou, Panagiota Bourtzis, Kostas Cladera, Jorge Luis Tsiamis, George Lanzavecchia, Silvia Beatriz Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title | Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title_full | Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title_fullStr | Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title_full_unstemmed | Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title_short | Gut bacteriome analysis of Anastrepha fraterculus sp. 1 during the early steps of laboratory colonization |
| title_sort | gut bacteriome analysis of anastrepha fraterculus sp 1 during the early steps of laboratory colonization |
| topic | Taxonomy Enterobacter Taxonomía Anastrepha fraterculus Sterile Insect Technique Bacterial Diversity Bacterial Richness Técnica del Insecto Estéril Diversidad Bacteriana Riqueza Bacteriana |
| url | http://hdl.handle.net/20.500.12123/8706 https://www.frontiersin.org/articles/10.3389/fmicb.2020.570960/full https://doi.org/10.3389/fmicb.2020.570960 |
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