A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine
Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model to predict their effectiveness in cattle. Immunostimulant Particle Adjuvant (ISP...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Frontiers Media
2021
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/8692 https://www.frontiersin.org/articles/10.3389/fvets.2020.00396/full https://doi.org/10.3389/fvets.2020.00396 |
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| author | Bidart, Juan Esteban Kornuta, Claudia Alejandra Gammella, Mariela Gnazzo, Victoria Soria, Ivana Langellotti, Cecilia Ana Mongini, Claudia Galarza, Roxana Ivon Calvinho, Luis Fernando Lupi, Giuliana Quattrocchi, Valeria Marcipar, Ivan Zamorano, Patricia Ines |
| author_browse | Bidart, Juan Esteban Calvinho, Luis Fernando Galarza, Roxana Ivon Gammella, Mariela Gnazzo, Victoria Kornuta, Claudia Alejandra Langellotti, Cecilia Ana Lupi, Giuliana Marcipar, Ivan Mongini, Claudia Quattrocchi, Valeria Soria, Ivana Zamorano, Patricia Ines |
| author_facet | Bidart, Juan Esteban Kornuta, Claudia Alejandra Gammella, Mariela Gnazzo, Victoria Soria, Ivana Langellotti, Cecilia Ana Mongini, Claudia Galarza, Roxana Ivon Calvinho, Luis Fernando Lupi, Giuliana Quattrocchi, Valeria Marcipar, Ivan Zamorano, Patricia Ines |
| author_sort | Bidart, Juan Esteban |
| collection | INTA Digital |
| description | Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model to predict their effectiveness in cattle. Immunostimulant Particle Adjuvant (ISPA), also known as cage-like particle adjuvant, consisting of lipid boxes of dipalmitoyl-phosphatidylcholine, cholesterol, sterylamine, alpha-tocopherol, and QuilA saponin, was shown to enhance protection of a recombinant vaccine against Trypanosoma cruzi in a mouse model. Thus, in the present work, we studied the effects on the magnitude and type of immunity elicited in mice and cattle in response to a vaccine based on inactivated FMD virus (iFMDV) formulated with ISPA. It was demonstrated that iFMDV–ISPA induced protection in mice against challenge and elicited a specific antibody response in sera, characterized by a balanced Th1/Th2 profile. In cattle, the antibody titers reached corresponded to an expected percentage of protection (EPP) higher than 80%. EPP calculates the probability that livestock would be protected against a 10,000 bovine infectious doses challenge after vaccination. Moreover, in comparison with the non-adjuvanted iFMDV vaccine, iFMDV–ISPA elicited an increased specific T-cell response against the virus, including higher interferon gamma (IFNγ)+/CD8+ lymphocyte production in cattle. In this work, we report for first time that an inactivated FMDV serotype A vaccine adjuvanted with ISPA is capable of inducing protection against challenge in a murine model and of improving the specific immune responses against the virus in cattle. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8692 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA86922021-02-19T11:36:03Z A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine Bidart, Juan Esteban Kornuta, Claudia Alejandra Gammella, Mariela Gnazzo, Victoria Soria, Ivana Langellotti, Cecilia Ana Mongini, Claudia Galarza, Roxana Ivon Calvinho, Luis Fernando Lupi, Giuliana Quattrocchi, Valeria Marcipar, Ivan Zamorano, Patricia Ines Foot and Mouth Disease Aphthovirus Immunostimulants Vaccine Adjuvants Adjuvants Protection Immune Response Cattle Fiebre Aftosa Virus Fiebre Aftosa Inmunoestimulantes Coadyuvantes de Vacunas Coadyuvantes Protección Respuesta Inmunológica Ganado Bovino Mouse Model Modelo de Ratón Foot-and-Mouth Disease (FMD) is an acute viral disease that causes important economy losses. Vaccines with new low-cost adjuvants that stimulate protective immune responses are needed and can be assayed in a mouse model to predict their effectiveness in cattle. Immunostimulant Particle Adjuvant (ISPA), also known as cage-like particle adjuvant, consisting of lipid boxes of dipalmitoyl-phosphatidylcholine, cholesterol, sterylamine, alpha-tocopherol, and QuilA saponin, was shown to enhance protection of a recombinant vaccine against Trypanosoma cruzi in a mouse model. Thus, in the present work, we studied the effects on the magnitude and type of immunity elicited in mice and cattle in response to a vaccine based on inactivated FMD virus (iFMDV) formulated with ISPA. It was demonstrated that iFMDV–ISPA induced protection in mice against challenge and elicited a specific antibody response in sera, characterized by a balanced Th1/Th2 profile. In cattle, the antibody titers reached corresponded to an expected percentage of protection (EPP) higher than 80%. EPP calculates the probability that livestock would be protected against a 10,000 bovine infectious doses challenge after vaccination. Moreover, in comparison with the non-adjuvanted iFMDV vaccine, iFMDV–ISPA elicited an increased specific T-cell response against the virus, including higher interferon gamma (IFNγ)+/CD8+ lymphocyte production in cattle. In this work, we report for first time that an inactivated FMDV serotype A vaccine adjuvanted with ISPA is capable of inducing protection against challenge in a murine model and of improving the specific immune responses against the virus in cattle. Instituto de Virología Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gnazzo, Victoria. Instituto Nacional de Medicina Tropical; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cuenca del Salado. Agencia de Extensión Rural Chascomús; Argentina Fil: Calvinho, Luis Fernando. INTA. Estación Experimental Agropecuaria Rafaela; Argentina Fil: Lupi, Giuliana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina 2021-02-19T11:25:37Z 2021-02-19T11:25:37Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8692 https://www.frontiersin.org/articles/10.3389/fvets.2020.00396/full 2297-1769 https://doi.org/10.3389/fvets.2020.00396 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 7 : 396 (Julio 2020) |
| spellingShingle | Foot and Mouth Disease Aphthovirus Immunostimulants Vaccine Adjuvants Adjuvants Protection Immune Response Cattle Fiebre Aftosa Virus Fiebre Aftosa Inmunoestimulantes Coadyuvantes de Vacunas Coadyuvantes Protección Respuesta Inmunológica Ganado Bovino Mouse Model Modelo de Ratón Bidart, Juan Esteban Kornuta, Claudia Alejandra Gammella, Mariela Gnazzo, Victoria Soria, Ivana Langellotti, Cecilia Ana Mongini, Claudia Galarza, Roxana Ivon Calvinho, Luis Fernando Lupi, Giuliana Quattrocchi, Valeria Marcipar, Ivan Zamorano, Patricia Ines A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title | A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title_full | A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title_fullStr | A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title_full_unstemmed | A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title_short | A new cage-like particle adjuvant enhances protection of foot-and-mouth disease vaccine |
| title_sort | new cage like particle adjuvant enhances protection of foot and mouth disease vaccine |
| topic | Foot and Mouth Disease Aphthovirus Immunostimulants Vaccine Adjuvants Adjuvants Protection Immune Response Cattle Fiebre Aftosa Virus Fiebre Aftosa Inmunoestimulantes Coadyuvantes de Vacunas Coadyuvantes Protección Respuesta Inmunológica Ganado Bovino Mouse Model Modelo de Ratón |
| url | http://hdl.handle.net/20.500.12123/8692 https://www.frontiersin.org/articles/10.3389/fvets.2020.00396/full https://doi.org/10.3389/fvets.2020.00396 |
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