Analysis of irradiated Argentinean fetal bovine serum for adventitious agents
Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Sage Publications
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8671 https://journals.sagepub.com/doi/abs/10.1177/1040638720951556 https://doi.org/10.1177/1040638720951556 |
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| author | Pecora, Andrea Perez Lopez, Jorgelina Jordan, Maximiliano Jesus Franco, Lautaro Nahuel Politzki, Romina Paula Ruiz, Vanesa Alvarez, Irene |
| author_browse | Alvarez, Irene Franco, Lautaro Nahuel Jordan, Maximiliano Jesus Pecora, Andrea Perez Lopez, Jorgelina Politzki, Romina Paula Ruiz, Vanesa |
| author_facet | Pecora, Andrea Perez Lopez, Jorgelina Jordan, Maximiliano Jesus Franco, Lautaro Nahuel Politzki, Romina Paula Ruiz, Vanesa Alvarez, Irene |
| author_sort | Pecora, Andrea |
| collection | INTA Digital |
| description | Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8671 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Sage Publications |
| publisherStr | Sage Publications |
| record_format | dspace |
| spelling | INTA86712021-02-17T16:44:47Z Analysis of irradiated Argentinean fetal bovine serum for adventitious agents Pecora, Andrea Perez Lopez, Jorgelina Jordan, Maximiliano Jesus Franco, Lautaro Nahuel Politzki, Romina Paula Ruiz, Vanesa Alvarez, Irene Argentina Contaminación Mycoplasma Virus Contamination Viruses Fetal Bovine Serum Adventitious Agents Pestiviruses Suero Fetal Bovino Agentes Adventicios Pestivirus Fetal bovine serum (FBS) used in cell culture may be contaminated with adventitious agents, which can affect the production of biologicals and the results of clinical laboratory tests. We carried out a retrospective study to determine the incidence of adventitious agent contamination of Argentinean irradiated FBS dating from 2015 to 2019. We analyzed FBS batches for mycoplasma and adventitious viruses (bovine pestiviruses, bovine adenovirus, bluetongue virus, bovine parainfluenza virus 3, rabies virus, bovine parvovirus, bovine herpesvirus 1, bovine respiratory syncytial virus, and reovirus). Cell passages followed by direct immunofluorescence were carried out to check viability of the mentioned adventitious agents. Also, molecular detection of mycoplasma and pestiviruses was performed on the FBS samples. The presence of neutralizing antibodies against pestiviruses was determined. Molecular analyses indicated that frequencies of mycoplasma and pestiviruses in FBS were 14% and 84%, respectively. All of the batches were seronegative for pestiviral antibodies. After cell passages, all FBS samples were negative for hemadsorbent agents and by immunofluorescence for all of the viral species analyzed; PCR assays were negative for mycoplasma and pestiviruses. Our results demonstrate that, of all adventitious agents tested, local FBS batches only had traces of mycoplasma and pestiviruses; gamma irradiation was effective in inactivating them. Instituto de Virología Fil: Pecora, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perez Lopez, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Jordan, Maximiliano Jesus. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Franco, Lautaro Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Politzki, Romina Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina 2021-02-17T16:31:29Z 2021-02-17T16:31:29Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8671 https://journals.sagepub.com/doi/abs/10.1177/1040638720951556 1943-4936 https://doi.org/10.1177/1040638720951556 eng info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. info:eu-repo/semantics/restrictedAccess application/pdf Sage Publications Journal of Veterinary Diagnostic Investigation 32 (6) : 892–897 (Noviembre 2020) |
| spellingShingle | Argentina Contaminación Mycoplasma Virus Contamination Viruses Fetal Bovine Serum Adventitious Agents Pestiviruses Suero Fetal Bovino Agentes Adventicios Pestivirus Pecora, Andrea Perez Lopez, Jorgelina Jordan, Maximiliano Jesus Franco, Lautaro Nahuel Politzki, Romina Paula Ruiz, Vanesa Alvarez, Irene Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title | Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title_full | Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title_fullStr | Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title_full_unstemmed | Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title_short | Analysis of irradiated Argentinean fetal bovine serum for adventitious agents |
| title_sort | analysis of irradiated argentinean fetal bovine serum for adventitious agents |
| topic | Argentina Contaminación Mycoplasma Virus Contamination Viruses Fetal Bovine Serum Adventitious Agents Pestiviruses Suero Fetal Bovino Agentes Adventicios Pestivirus |
| url | http://hdl.handle.net/20.500.12123/8671 https://journals.sagepub.com/doi/abs/10.1177/1040638720951556 https://doi.org/10.1177/1040638720951556 |
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