Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants
Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highl...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley-Blackwell
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8655 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640289/ https://doi.org/10.1111/j.1364-3703.2008.00469.x |
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| author | Almasia, Natalia Ines Bazzini, Ariel Alejandro Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Bazzini, Ariel Alejandro Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_facet | Almasia, Natalia Ines Bazzini, Ariel Alejandro Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_sort | Almasia, Natalia Ines |
| collection | INTA Digital |
| description | Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping‐off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild‐type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8655 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley-Blackwell |
| publisherStr | Wiley-Blackwell |
| record_format | dspace |
| spelling | INTA86552021-01-27T13:34:36Z Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants Almasia, Natalia Ines Bazzini, Ariel Alejandro Hopp, Horacio Esteban Vazquez Rovere, Cecilia Disease Resistance Transgenic Plants Potatoes Resistencia a la Enfermedad Rhizoctonia Solani Pectobacterium Carotovorum Plantas Transgénicas Papa Solanum Erwinia Carotovora Snakin‐1 (SN1), a cysteine‐rich peptide with broad‐spectrum antimicrobial activity in vitro, was evaluated for its ability to confer resistance to pathogens in transgenic potatoes. Genetic variants of this gene were cloned from wild and cultivated Solanum species. Nucleotide sequences revealed highly evolutionary conservation with 91–98% identity values. Potato plants (S. tuberosum subsp. tuberosum cv. Kennebec) were transformed via Agrobacterium tumefaciens with a construct encoding the S. chacoense SN1 gene under the regulation of the ubiquitous CaMV 35S promoter. Transgenic lines were molecularly characterized and challenged with either Rhizoctonia solani or Erwinia carotovora to analyse whether constitutive in vivo overexpression of the SN1 gene may lead to disease resistance. Only transgenic lines that accumulated high levels of SN1 mRNA exhibited significant symptom reductions of R. solani infection such as stem cankers and damping‐off. Furthermore, these overexpressing lines showed significantly higher survival rates throughout the fungal resistance bioassays. In addition, the same lines showed significant protection against E. carotovora measured as: a reduction of lesion areas (from 46.5 to 88.1% with respect to the wild‐type), number of fallen leaves and thickened or necrotic stems. Enhanced resistance to these two important potato pathogens suggests in vivo antifungal and antibacterial activity of SN1 and thus its possible biotechnological application. Instituto de Biotecnología Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Almasia, Natalia Ines. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Fisiología, Biología Molecular y Celular. Área de Biotecnología; Argentina 2021-01-27T13:23:19Z 2021-01-27T13:23:19Z 2008-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8655 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640289/ 1364-3703 https://doi.org/10.1111/j.1364-3703.2008.00469.x eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley-Blackwell Molecular plant pathology 9 (3) : 329-338. (Mayo 2008) |
| spellingShingle | Disease Resistance Transgenic Plants Potatoes Resistencia a la Enfermedad Rhizoctonia Solani Pectobacterium Carotovorum Plantas Transgénicas Papa Solanum Erwinia Carotovora Almasia, Natalia Ines Bazzini, Ariel Alejandro Hopp, Horacio Esteban Vazquez Rovere, Cecilia Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title | Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title_full | Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title_fullStr | Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title_full_unstemmed | Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title_short | Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants |
| title_sort | overexpression of snakin 1 gene enhances resistance to rhizoctonia solani and erwinia carotovora in transgenic potato plants |
| topic | Disease Resistance Transgenic Plants Potatoes Resistencia a la Enfermedad Rhizoctonia Solani Pectobacterium Carotovorum Plantas Transgénicas Papa Solanum Erwinia Carotovora |
| url | http://hdl.handle.net/20.500.12123/8655 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6640289/ https://doi.org/10.1111/j.1364-3703.2008.00469.x |
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