New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera
Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily we...
| Main Authors: | , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8652 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000011 https://doi.org/10.1016/j.cimid.2021.101609 |
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| author | Martinez, Mara Leila Rodriguez, Marcelo A. Irazu, Lucía E. Romero, Graciela Noemi Saraullo, Vanina Rosa Watanabe, Olivia Hamer, Micaela Grune Loffler, Sylvia Samartino, Luis Ernesto Brihuega, Bibiana Felicitas |
| author_browse | Brihuega, Bibiana Felicitas Grune Loffler, Sylvia Hamer, Micaela Irazu, Lucía E. Martinez, Mara Leila Rodriguez, Marcelo A. Romero, Graciela Noemi Samartino, Luis Ernesto Saraullo, Vanina Rosa Watanabe, Olivia |
| author_facet | Martinez, Mara Leila Rodriguez, Marcelo A. Irazu, Lucía E. Romero, Graciela Noemi Saraullo, Vanina Rosa Watanabe, Olivia Hamer, Micaela Grune Loffler, Sylvia Samartino, Luis Ernesto Brihuega, Bibiana Felicitas |
| author_sort | Martinez, Mara Leila |
| collection | INTA Digital |
| description | Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine. |
| format | Artículo |
| id | INTA8652 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA86522021-01-26T15:33:50Z New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera Martinez, Mara Leila Rodriguez, Marcelo A. Irazu, Lucía E. Romero, Graciela Noemi Saraullo, Vanina Rosa Watanabe, Olivia Hamer, Micaela Grune Loffler, Sylvia Samartino, Luis Ernesto Brihuega, Bibiana Felicitas ELISA Bovinae Leptospira Leptospirosis Diagnóstico Aborto Enzimas Técnicas Inmunológicas Anticuerpos Diagnosis Abortion Enzymes Immunological Techniques Antibodies Leptospirosis is a zoonotic disease with worldwide endemicity in Argentina, it is a significant public health problem in low-income populations. Bovine leptospirosis is a serious economic problem for cattle production, causing abortions, reduced milk yield, mortality in calves and decreased daily weight gain. We developed an enzyme-linked immunosorbent assay (ELISA) with sonicated Leptospira interrogans serogroup Icterohaemorrhagiae serovar Copenhageni M 20. We evaluated its performance for the detection of specific antibodies against multiple Leptospira serogroups in bovine. The microscopic agglutination test (MAT) was used as the gold standard. The performance of this ELISA was evaluated with a panel of sera (118 MAT confirmed positive and 97 MAT negative). The overall sensitivity was close to 85.6 % and the specificity was 83.5 %, according to the MAT reference method. Analytical specificity of the IgG-ELISA was evaluated using 50 bovine serum samples from animals showing serum antibodies against other pathogens that cause abortion in bovine, such as Brucella sp., Neospora sp. and Bovine Viral Diarrhea (BVD), and no cross-reaction was observed. This IgG-ELISA can be an alternative to the MAT for diagnosis of leptospiral infection in bovine. Instituto de Patobiología Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Irazu, Lucía E. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina Fil: Romero, Graciela Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Watanabe, Olivia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Grune Loffler, Sylvia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Samartino, Luis Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero, Graciela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Watanabe, Olivia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Grune Loffler, Sylvia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Samartino, Luis Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-01-26T15:01:16Z 2021-01-26T15:01:16Z 2021-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8652 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000011 1878-1667 https://doi.org/10.1016/j.cimid.2021.101609 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Comparative immunology, microbiology and infectious ddiseases 75 : 101609. (Abril 2021) |
| spellingShingle | ELISA Bovinae Leptospira Leptospirosis Diagnóstico Aborto Enzimas Técnicas Inmunológicas Anticuerpos Diagnosis Abortion Enzymes Immunological Techniques Antibodies Martinez, Mara Leila Rodriguez, Marcelo A. Irazu, Lucía E. Romero, Graciela Noemi Saraullo, Vanina Rosa Watanabe, Olivia Hamer, Micaela Grune Loffler, Sylvia Samartino, Luis Ernesto Brihuega, Bibiana Felicitas New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title | New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title_full | New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title_fullStr | New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title_full_unstemmed | New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title_short | New enzyme-linked immunoassay for the detection of specific antibodies against multiple Leptospira serogroups in bovine sera |
| title_sort | new enzyme linked immunoassay for the detection of specific antibodies against multiple leptospira serogroups in bovine sera |
| topic | ELISA Bovinae Leptospira Leptospirosis Diagnóstico Aborto Enzimas Técnicas Inmunológicas Anticuerpos Diagnosis Abortion Enzymes Immunological Techniques Antibodies |
| url | http://hdl.handle.net/20.500.12123/8652 https://www.sciencedirect.com/science/article/abs/pii/S0147957121000011 https://doi.org/10.1016/j.cimid.2021.101609 |
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