Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction
Anastrepha fraterculus sp. 1 is considered a quarantine pest in several American countries. Since chemical control applied in an integrated pest management program is the only strategy utilized against this pest, the development of pesticide-free methods, such as the Sterile Insect Technique, is bei...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BioMed Central
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8644 https://bmcgenomdata.biomedcentral.com/articles/10.1186/s12863-020-00943-2 https://doi.org/10.1186/s12863-020-00943-2 |
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| author | Scannapieco, Alejandra Carla Conte, Claudia Alejandra Rivarola, Maximo Lisandro Wulff, Juan Pedro Muntaabski, Irina Ribone, Andrés Ignacio Milla, Fabian Horacio Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_browse | Cladera, Jorge Luis Conte, Claudia Alejandra Lanzavecchia, Silvia Beatriz Milla, Fabian Horacio Muntaabski, Irina Ribone, Andrés Ignacio Rivarola, Maximo Lisandro Scannapieco, Alejandra Carla Wulff, Juan Pedro |
| author_facet | Scannapieco, Alejandra Carla Conte, Claudia Alejandra Rivarola, Maximo Lisandro Wulff, Juan Pedro Muntaabski, Irina Ribone, Andrés Ignacio Milla, Fabian Horacio Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz |
| author_sort | Scannapieco, Alejandra Carla |
| collection | INTA Digital |
| description | Anastrepha fraterculus sp. 1 is considered a quarantine pest in several American countries. Since chemical control applied in an integrated pest management program is the only strategy utilized against this pest, the development of pesticide-free methods, such as the Sterile Insect Technique, is being considered. The search for genes involved in sex-determination and differentiation, and in metabolic pathways associated with communication and mating behaviour, contributes with key information to the development of genetic control strategies. The aims of this work were to perform a comprehensive analysis of A. fraterculus sp. 1 transcriptome and to obtain an initial evaluation of genes associated with main metabolic pathways by the expression analysis of specific transcripts identified in embryos and adults.
Results
Sexually mature adults of both sexes and 72 h embryos were considered for transcriptome analysis. The de novo transcriptome assembly was fairly complete (62.9% complete BUSCO orthologs detected) with a total of 86,925 transcripts assembled and 28,756 GO annotated sequences. Paired-comparisons between libraries showed 319 transcripts differently expressed between embryos and females, 1242 between embryos and males, and 464 between sexes. Using this information and genes searches based on published studies from other tephritid species, we evaluated a set of transcripts involved in development, courtship and metabolic pathways. The qPCR analysis evidenced that the early genes serendipity alpha and transformer-2 displayed similar expression levels in the analyzed stages, while heat shock protein 27 is over-expressed in embryos and females in comparison to males. The expression of genes associated with courtship (takeout-like, odorant-binding protein 50a1) differed between males and females, independently of their reproductive status (virgin vs mated individuals). Genes associated with metabolic pathways (maltase 2-like, androgen-induced gene 1) showed differential expression between embryos and adults. Furthermore, 14,262 microsatellite motifs were identified, with 11,208 transcripts containing at least one simple sequence repeat, including 48% of di/trinucleotide motifs.
Conclusion
Our results significantly expand the available gene space of A. fraterculus sp. 1, contributing with a fairly complete transcript database of embryos and adults. The expression analysis of the selected candidate genes, along with a set of microsatellite markers, provides a valuable resource for further genetic characterization of A. fraterculus sp. 1 and supports the development of specific genetic control strategies. |
| format | Artículo |
| id | INTA8644 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA86442021-01-25T17:13:39Z Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction Scannapieco, Alejandra Carla Conte, Claudia Alejandra Rivarola, Maximo Lisandro Wulff, Juan Pedro Muntaabski, Irina Ribone, Andrés Ignacio Milla, Fabian Horacio Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Tephritidae Secuencia de ARN Transcripción Expresión Génica Microsatélites Anastrepha Fraterculus RNA Sequence Transcription Gene Expression Microsatellites Anastrepha fraterculus sp. 1 is considered a quarantine pest in several American countries. Since chemical control applied in an integrated pest management program is the only strategy utilized against this pest, the development of pesticide-free methods, such as the Sterile Insect Technique, is being considered. The search for genes involved in sex-determination and differentiation, and in metabolic pathways associated with communication and mating behaviour, contributes with key information to the development of genetic control strategies. The aims of this work were to perform a comprehensive analysis of A. fraterculus sp. 1 transcriptome and to obtain an initial evaluation of genes associated with main metabolic pathways by the expression analysis of specific transcripts identified in embryos and adults. Results Sexually mature adults of both sexes and 72 h embryos were considered for transcriptome analysis. The de novo transcriptome assembly was fairly complete (62.9% complete BUSCO orthologs detected) with a total of 86,925 transcripts assembled and 28,756 GO annotated sequences. Paired-comparisons between libraries showed 319 transcripts differently expressed between embryos and females, 1242 between embryos and males, and 464 between sexes. Using this information and genes searches based on published studies from other tephritid species, we evaluated a set of transcripts involved in development, courtship and metabolic pathways. The qPCR analysis evidenced that the early genes serendipity alpha and transformer-2 displayed similar expression levels in the analyzed stages, while heat shock protein 27 is over-expressed in embryos and females in comparison to males. The expression of genes associated with courtship (takeout-like, odorant-binding protein 50a1) differed between males and females, independently of their reproductive status (virgin vs mated individuals). Genes associated with metabolic pathways (maltase 2-like, androgen-induced gene 1) showed differential expression between embryos and adults. Furthermore, 14,262 microsatellite motifs were identified, with 11,208 transcripts containing at least one simple sequence repeat, including 48% of di/trinucleotide motifs. Conclusion Our results significantly expand the available gene space of A. fraterculus sp. 1, contributing with a fairly complete transcript database of embryos and adults. The expression analysis of the selected candidate genes, along with a set of microsatellite markers, provides a valuable resource for further genetic characterization of A. fraterculus sp. 1 and supports the development of specific genetic control strategies. Instituto de Genética Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Rivarola, Maximo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Wulff, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Económica; Argentina. Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Ribone, Andrés Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Genética de Insectos de Importancia Económica; Argentina. Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina. Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Maximo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Wulff, Juan Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Wulff, Juan Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ribone, Andrés Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cladera, Jorge Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Cladera, Jorge Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-01-25T16:55:17Z 2021-01-25T16:55:17Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8644 https://bmcgenomdata.biomedcentral.com/articles/10.1186/s12863-020-00943-2 1471-2156 https://doi.org/10.1186/s12863-020-00943-2 eng info:eu-repograntAgreement/INTA/PNBIO-1131044/AR./Genómica aplicada a estudios de ecología molecular y diversidad genética. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC Genetics 21 (Suppl 2) : 136. (Diciembre 2020) |
| spellingShingle | Tephritidae Secuencia de ARN Transcripción Expresión Génica Microsatélites Anastrepha Fraterculus RNA Sequence Transcription Gene Expression Microsatellites Scannapieco, Alejandra Carla Conte, Claudia Alejandra Rivarola, Maximo Lisandro Wulff, Juan Pedro Muntaabski, Irina Ribone, Andrés Ignacio Milla, Fabian Horacio Cladera, Jorge Luis Lanzavecchia, Silvia Beatriz Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title | Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title_full | Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title_fullStr | Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title_full_unstemmed | Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title_short | Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction |
| title_sort | transcriptome analysis of anastrepha fraterculus sp 1 males females and embryos insights into development courtship and reproduction |
| topic | Tephritidae Secuencia de ARN Transcripción Expresión Génica Microsatélites Anastrepha Fraterculus RNA Sequence Transcription Gene Expression Microsatellites |
| url | http://hdl.handle.net/20.500.12123/8644 https://bmcgenomdata.biomedcentral.com/articles/10.1186/s12863-020-00943-2 https://doi.org/10.1186/s12863-020-00943-2 |
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