Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding
Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Pontificia Universidad Catolica de Valparaiso
2021
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8640 https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso |
| _version_ | 1855484239215067136 |
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| author | Almasia, Natalia Ines Nahirñak, Vanesa Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_browse | Almasia, Natalia Ines Hopp, Horacio Esteban Nahirñak, Vanesa Vazquez Rovere, Cecilia |
| author_facet | Almasia, Natalia Ines Nahirñak, Vanesa Hopp, Horacio Esteban Vazquez Rovere, Cecilia |
| author_sort | Almasia, Natalia Ines |
| collection | INTA Digital |
| description | Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering. |
| format | Artículo |
| id | INTA8640 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Pontificia Universidad Catolica de Valparaiso |
| publisherStr | Pontificia Universidad Catolica de Valparaiso |
| record_format | dspace |
| spelling | INTA86402021-01-22T18:06:17Z Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding Almasia, Natalia Ines Nahirñak, Vanesa Hopp, Horacio Esteban Vazquez Rovere, Cecilia Peptides Antimicrobials Potatoes Genetic Isolation Temperature Transgenic Plants Péptidos Antimicrobianos Papa Solanum Tuberosum Aislamiento Genético Temperatura Plantas Transgénicas Snakin-1 (StSN1) is a broad-spectrum antimicrobial peptide isolated from Solanum tuberosum. Homologous proteins have been identified in a wide range of species but there is no apparent consensus in the roles they play. A 1394 bp fragment of the 5’upstream region of StSN1 gene, designated PStSN1, was isolated from the potato genome and sequenced. Bioinformatics analyses revealed a total of 55 potential regulatory motifs related to tissue-specificity, stress, defence and hormone responsiveness, among others. PStSN1 spatial and temporal activity was studied in transgenic Arabidopsis plants expressing a reporter gene under this promoter control (PStSN1::GUS). Histochemical staining revealed PStSN1::GUS expression in the root vasculature, cotyledons, young leaves and floral organs. Moreover, GUS staining was detected in young developmental stages gradually decreasing as the plant aged. Stress treatments on transgenic plants showed that PStSN1 activity was induced by high/low temperature and wounding. The characterization of PStSN1 in a model plant establishes a framework for the understanding of its possible biological functions and provides a potential tool for plant modification through genetic engineering. Instituto de Biotecnología Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Nahirñak, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Vazquez Rovere, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2021-01-22T18:01:10Z 2021-01-22T18:01:10Z 2010-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8640 https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso 0717-3458 10.2225/vol13-issue5-fulltext-12 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Pontificia Universidad Catolica de Valparaiso Electronic journal of biotechnology 13 (5) : 1-21. (Septiembre 2010) |
| spellingShingle | Peptides Antimicrobials Potatoes Genetic Isolation Temperature Transgenic Plants Péptidos Antimicrobianos Papa Solanum Tuberosum Aislamiento Genético Temperatura Plantas Transgénicas Almasia, Natalia Ines Nahirñak, Vanesa Hopp, Horacio Esteban Vazquez Rovere, Cecilia Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title | Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title_full | Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title_fullStr | Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title_full_unstemmed | Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title_short | Isolation and characterization of the tissue and development-specific potato snakin-1 promoter inducible by temperature and wounding |
| title_sort | isolation and characterization of the tissue and development specific potato snakin 1 promoter inducible by temperature and wounding |
| topic | Peptides Antimicrobials Potatoes Genetic Isolation Temperature Transgenic Plants Péptidos Antimicrobianos Papa Solanum Tuberosum Aislamiento Genético Temperatura Plantas Transgénicas |
| url | http://hdl.handle.net/20.500.12123/8640 https://scielo.conicyt.cl/scielo.php?script=sci_abstract&pid=S0717-34582010000500008&lng=es&nrm=iso |
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