Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR
Ascochyta blight is the major disease affecting chickpea (Cicer arietinum) around the world. Since the first report of Ascochyta rabiei's isolation in Argentina in 2012, the pathogen has caused severe economic losses in crop production; so, the detection and rapid identification of the pathogen in e...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8639 https://www.sciencedirect.com/science/article/pii/S0261219420303963 https://doi.org/10.1016/j.cropro.2020.105463 |
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| author | Valetti, Lucio Cazon, Luis Ignacio Crociara, Clara Sonia Pastor, Silvina Estela |
| author_browse | Cazon, Luis Ignacio Crociara, Clara Sonia Pastor, Silvina Estela Valetti, Lucio |
| author_facet | Valetti, Lucio Cazon, Luis Ignacio Crociara, Clara Sonia Pastor, Silvina Estela |
| author_sort | Valetti, Lucio |
| collection | INTA Digital |
| description | Ascochyta blight is the major disease affecting chickpea (Cicer arietinum) around the world. Since the first report of Ascochyta rabiei's isolation in Argentina in 2012, the pathogen has caused severe economic losses in crop production; so, the detection and rapid identification of the pathogen in early stages is key for the management of the disease. In this work, a traditional PCR procedure for detection of A. rabiei directly from plant tissues has been described based on beta-tubulin gene. The TP-6/TP-9 specific primers designed, amplified only a single PCR band of 770 bp from A. rabiei. The specificity of the primers was checked using 12 isolates of A. rabiei and DNA from 10 other different fungi including common pathogens of chickpea as Alternaria alternata, Botrytis cinerea, Sclerotinia sclerotiorum and Phoma medicaginis that cause similar symptoms. The detection sensitivity with primers was 2 × 104 ng μl−1 genomic DNA. In inoculated plant material, PCR amplification gave a band of the expected size and no amplification was observed when DNA was from healthy and uninoculated plants. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods. The PCR-based method developed here can simplify both plant disease diagnosis, and pathogen monitoring in an early phase, as well as aid in effective management practices that avoid the disease advance and minimize losses. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8639 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA86392021-01-22T17:59:07Z Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR Valetti, Lucio Cazon, Luis Ignacio Crociara, Clara Sonia Pastor, Silvina Estela Ascochyta Rabiei Garbanzo Diagnostic Techniques Ascochyta Blight on Peas Chickpeas Técnicas de Diagnosis PCR Molecular Diagnostic Ascochyta Blight Chickpea Ascochyta blight is the major disease affecting chickpea (Cicer arietinum) around the world. Since the first report of Ascochyta rabiei's isolation in Argentina in 2012, the pathogen has caused severe economic losses in crop production; so, the detection and rapid identification of the pathogen in early stages is key for the management of the disease. In this work, a traditional PCR procedure for detection of A. rabiei directly from plant tissues has been described based on beta-tubulin gene. The TP-6/TP-9 specific primers designed, amplified only a single PCR band of 770 bp from A. rabiei. The specificity of the primers was checked using 12 isolates of A. rabiei and DNA from 10 other different fungi including common pathogens of chickpea as Alternaria alternata, Botrytis cinerea, Sclerotinia sclerotiorum and Phoma medicaginis that cause similar symptoms. The detection sensitivity with primers was 2 × 104 ng μl−1 genomic DNA. In inoculated plant material, PCR amplification gave a band of the expected size and no amplification was observed when DNA was from healthy and uninoculated plants. The results suggested that the assay detected the pathogen more rapidly and accurately than standard isolation methods. The PCR-based method developed here can simplify both plant disease diagnosis, and pathogen monitoring in an early phase, as well as aid in effective management practices that avoid the disease advance and minimize losses. Instituto de Patología Vegetal Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Patología Vegetal (IPAVE). Córdoba; Argentina. Fil: Cazón, Luis Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Patología Vegetal (IPAVE). Córdoba; Argentina. Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFYMA).Córdoba; Argentina Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigaciones Agropecuarias (CIAP). Instituto de Patología Vegetal (IPAVE). Córdoba; Argentina. Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET). Unidad de Fitopatología y Modelización Agrícola (UFYMA).Córdoba; Argentina. Fil: Cazón, Luis Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFYMA).Córdoba; Argentina. Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFYMA).Córdoba; Argentina. 2021-01-22T17:44:39Z 2021-01-22T17:44:39Z 2020-11-16 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8639 https://www.sciencedirect.com/science/article/pii/S0261219420303963 0261-2194 https://doi.org/10.1016/j.cropro.2020.105463 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Crop Protection : 105463. (16 November 2020) |
| spellingShingle | Ascochyta Rabiei Garbanzo Diagnostic Techniques Ascochyta Blight on Peas Chickpeas Técnicas de Diagnosis PCR Molecular Diagnostic Ascochyta Blight Chickpea Valetti, Lucio Cazon, Luis Ignacio Crociara, Clara Sonia Pastor, Silvina Estela Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title | Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title_full | Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title_fullStr | Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title_full_unstemmed | Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title_short | Early detection of Ascochyta blight (Ascochyta rabiei) of chickpea by traditional PCR |
| title_sort | early detection of ascochyta blight ascochyta rabiei of chickpea by traditional pcr |
| topic | Ascochyta Rabiei Garbanzo Diagnostic Techniques Ascochyta Blight on Peas Chickpeas Técnicas de Diagnosis PCR Molecular Diagnostic Ascochyta Blight Chickpea |
| url | http://hdl.handle.net/20.500.12123/8639 https://www.sciencedirect.com/science/article/pii/S0261219420303963 https://doi.org/10.1016/j.cropro.2020.105463 |
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