Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina

Varroa destructor is a serious ectoparasite of the western honey bee, Apis mellifera, which negatively impacts on colonies health and survival worldwide. Drone-mediated movement and the presence of the mite in Drone Congregation Areas (DCA) may play a relevant role in Varroa dispersal. The objective...

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Autores principales: Galindo Cardona, Alberto, Scannapieco, Alejandra Carla, Russo, Romina Maria, Escalante, Karen, Geria, Martín, Lepori, Nicolás, Ayup, María Marta, Muntaabski, Irina, Liendo, María Clara, Landi, Lucas, Giray, Tugrul, Monmany-Garzia, A. Carolina
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Frontiers Media 2021
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/8621
https://www.frontiersin.org/articles/10.3389/fevo.2020.590345/full
https://doi.org/10.3389/fevo.2020.590345
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author Galindo Cardona, Alberto
Scannapieco, Alejandra Carla
Russo, Romina Maria
Escalante, Karen
Geria, Martín
Lepori, Nicolás
Ayup, María Marta
Muntaabski, Irina
Liendo, María Clara
Landi, Lucas
Giray, Tugrul
Monmany-Garzia, A. Carolina
author_browse Ayup, María Marta
Escalante, Karen
Galindo Cardona, Alberto
Geria, Martín
Giray, Tugrul
Landi, Lucas
Lepori, Nicolás
Liendo, María Clara
Monmany-Garzia, A. Carolina
Muntaabski, Irina
Russo, Romina Maria
Scannapieco, Alejandra Carla
author_facet Galindo Cardona, Alberto
Scannapieco, Alejandra Carla
Russo, Romina Maria
Escalante, Karen
Geria, Martín
Lepori, Nicolás
Ayup, María Marta
Muntaabski, Irina
Liendo, María Clara
Landi, Lucas
Giray, Tugrul
Monmany-Garzia, A. Carolina
author_sort Galindo Cardona, Alberto
collection INTA Digital
description Varroa destructor is a serious ectoparasite of the western honey bee, Apis mellifera, which negatively impacts on colonies health and survival worldwide. Drone-mediated movement and the presence of the mite in Drone Congregation Areas (DCA) may play a relevant role in Varroa dispersal. The objectives of this study were to characterize mite infestation levels and genetic diversity in DCAs and surrounding apiaries and to explore putative environmental variables associated to Varroa infestation in two eco-climatic regions of Argentina (temperate, and subtropical). Phoretic mite proportions in DCAs and apiaries were estimated during spring. Landscape, topographic, and climate variables were described using satellite image classifications and data from public databases. The genetic composition of drones at the DCAs and workers from the surrounding apiaries was assessed using mitochondrial markers. In total, eleven DCAs were identified in both regions during 2017 and 2018. The mean proportion of Varroa was ca. 3 in 1,000 (0.0028 ± 0.0046) at the apiaries, and ca. 2 in 100 (0.0168 ± 0.0227) at the DCAs. No statistical differences were observed between apiaries and DCAs or between ecoregions, but the proportion of infested males at the DCAs was positively correlated to the distance to the apiary and a trend was observed toward higher mite loads in DCAs. Landscape and topography were not determinant for Varroa infestation at the DCAs but relative humidity and precipitation in the previous week of sampling, positively influenced infestation. More haplotypic diversity was detected in the DCAs compared to the surrounding apiaries, particularly in the subtropical region. While in this region high prevalence of Africanized (A1, A4) mitochondrial lineages was detected, European lineages (C1, C2j) were mostly found in apiaries and DCA in the temperate region. Our results provide valuable information on the dynamics of Varroa parasitism in apiaries and DCAs, and highlight the role of drones in mite dispersion and genetic variability of new colonies. The study of DCAs emerges as a tool for investigating not only honey bee reproduction and conservation, but also the impact of the environment on bee epidemiology.
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spelling INTA86212021-01-20T13:08:08Z Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina Galindo Cardona, Alberto Scannapieco, Alejandra Carla Russo, Romina Maria Escalante, Karen Geria, Martín Lepori, Nicolás Ayup, María Marta Muntaabski, Irina Liendo, María Clara Landi, Lucas Giray, Tugrul Monmany-Garzia, A. Carolina Drones (Insects) Zánganos Argentina Variación genética Parasitismo Abeja Melífera Apis Mellifera Genetic variation Parasitism Varroa Destructor Honey Bees Drone Congregation Areas Área de Congregación de Drones Ecoclimatic Regions Regiones Ecoclimáticas Varroa destructor is a serious ectoparasite of the western honey bee, Apis mellifera, which negatively impacts on colonies health and survival worldwide. Drone-mediated movement and the presence of the mite in Drone Congregation Areas (DCA) may play a relevant role in Varroa dispersal. The objectives of this study were to characterize mite infestation levels and genetic diversity in DCAs and surrounding apiaries and to explore putative environmental variables associated to Varroa infestation in two eco-climatic regions of Argentina (temperate, and subtropical). Phoretic mite proportions in DCAs and apiaries were estimated during spring. Landscape, topographic, and climate variables were described using satellite image classifications and data from public databases. The genetic composition of drones at the DCAs and workers from the surrounding apiaries was assessed using mitochondrial markers. In total, eleven DCAs were identified in both regions during 2017 and 2018. The mean proportion of Varroa was ca. 3 in 1,000 (0.0028 ± 0.0046) at the apiaries, and ca. 2 in 100 (0.0168 ± 0.0227) at the DCAs. No statistical differences were observed between apiaries and DCAs or between ecoregions, but the proportion of infested males at the DCAs was positively correlated to the distance to the apiary and a trend was observed toward higher mite loads in DCAs. Landscape and topography were not determinant for Varroa infestation at the DCAs but relative humidity and precipitation in the previous week of sampling, positively influenced infestation. More haplotypic diversity was detected in the DCAs compared to the surrounding apiaries, particularly in the subtropical region. While in this region high prevalence of Africanized (A1, A4) mitochondrial lineages was detected, European lineages (C1, C2j) were mostly found in apiaries and DCA in the temperate region. Our results provide valuable information on the dynamics of Varroa parasitism in apiaries and DCAs, and highlight the role of drones in mite dispersion and genetic variability of new colonies. The study of DCAs emerges as a tool for investigating not only honey bee reproduction and conservation, but also the impact of the environment on bee epidemiology. Instituto de Genética Fil: Galindo Cardona, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Russo, Romina María. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Escalante, Karen. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina Fil: Geria, Martín. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina Fil: Lepori, Nicolás. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina Fil: Ayup, María Marta. Universidad Nacional de Tucumán. Instituto de Ecología Regional; Argentina. Fil: Muntaabski, Irina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Fil: Landi, Lucas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Giray, Tugrul. Universidad de Puerto Rico. Departamento de Biología; Puerto Rico Fil: Monmany-Garzia, A. Carolina. Universidad Nacional de Tucumán. Instituto de Ecología Regional; Argentina Fil: Galindo Cardona, Alberto. Fundación Miguel Lillo; Argentina. Fil: Galindo Cardona, Alberto. Universidad Nacional de Tucumán. Instituto de Ecología Regional; Argentina Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ayup, María Marta. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales; Argentina Fil: Muntaabski, Irina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-01-20T12:52:42Z 2021-01-20T12:52:42Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8621 https://www.frontiersin.org/articles/10.3389/fevo.2020.590345/full 2296-701X https://doi.org/10.3389/fevo.2020.590345 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 Frontiers Media Frontiers in ecology and evolution 8 : 590345. (Noviembre 2020)
spellingShingle Drones (Insects)
Zánganos
Argentina
Variación genética
Parasitismo
Abeja Melífera
Apis Mellifera
Genetic variation
Parasitism
Varroa Destructor
Honey Bees
Drone Congregation Areas
Área de Congregación de Drones
Ecoclimatic Regions
Regiones Ecoclimáticas
Galindo Cardona, Alberto
Scannapieco, Alejandra Carla
Russo, Romina Maria
Escalante, Karen
Geria, Martín
Lepori, Nicolás
Ayup, María Marta
Muntaabski, Irina
Liendo, María Clara
Landi, Lucas
Giray, Tugrul
Monmany-Garzia, A. Carolina
Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title_full Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title_fullStr Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title_full_unstemmed Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title_short Varroa destructor parasitism and genetic variability at honey bee (Apis mellifera) drone congregation areas and their associations with environmental variables in Argentina
title_sort varroa destructor parasitism and genetic variability at honey bee apis mellifera drone congregation areas and their associations with environmental variables in argentina
topic Drones (Insects)
Zánganos
Argentina
Variación genética
Parasitismo
Abeja Melífera
Apis Mellifera
Genetic variation
Parasitism
Varroa Destructor
Honey Bees
Drone Congregation Areas
Área de Congregación de Drones
Ecoclimatic Regions
Regiones Ecoclimáticas
url http://hdl.handle.net/20.500.12123/8621
https://www.frontiersin.org/articles/10.3389/fevo.2020.590345/full
https://doi.org/10.3389/fevo.2020.590345
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