MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV...
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| Format: | Artículo |
| Language: | Inglés |
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Wiley
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/8603 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13718 https://doi.org/10.1111/tbed.13718 |
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| author | Kornuta, Claudia Alejandra Bidart, , Juan Esteban Soria, Ivana Gammella, Mariela Quattrocchi, Valeria Pappalardo, Juan Sebastián Salmaso, Stefano Torchilin, Vladimir P. Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Dadin P. Zamorano, Patricia Ines Langellotti, Cecilia Ana |
| author_browse | Bidart, , Juan Esteban Cheuquepan Valenzuel, Felipe Andres Gammella, Mariela Hecker, Yanina Kornuta, Claudia Alejandra Langellotti, Cecilia Ana Moore, Dadin P. Pappalardo, Juan Sebastián Quattrocchi, Valeria Salmaso, Stefano Soria, Ivana Torchilin, Vladimir P. Zamorano, Patricia Ines |
| author_facet | Kornuta, Claudia Alejandra Bidart, , Juan Esteban Soria, Ivana Gammella, Mariela Quattrocchi, Valeria Pappalardo, Juan Sebastián Salmaso, Stefano Torchilin, Vladimir P. Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Dadin P. Zamorano, Patricia Ines Langellotti, Cecilia Ana |
| author_sort | Kornuta, Claudia Alejandra |
| collection | INTA Digital |
| description | New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis. |
| format | Artículo |
| id | INTA8603 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA86032023-01-13T13:56:12Z MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 Kornuta, Claudia Alejandra Bidart, , Juan Esteban Soria, Ivana Gammella, Mariela Quattrocchi, Valeria Pappalardo, Juan Sebastián Salmaso, Stefano Torchilin, Vladimir P. Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Dadin P. Zamorano, Patricia Ines Langellotti, Cecilia Ana Liposomes (Organelles) Liposomas (Organulos) Inmunogenética Vacuna Herpesviridae Immunogenetics Vaccines New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis. Instituto de Virología Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina Fil: Salmaso, Stefano. Universita degli Studi di Padova. Department of Pharmaceutical and Pharmacological Sciences; Italia Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Kornuta, Claudia Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Gammella, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina Fil: Zamorano, Patricia Ines. Universidad del Salvador; Argentina Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina 2021-01-15T13:48:31Z 2021-01-15T13:48:31Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8603 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13718 1865-1674 https://doi.org/10.1111/tbed.13718 eng info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. info:eu-repo/semantics/restrictedAccess application/pdf Wiley Transboundary and emerging diseases 68 (2) : 587-597 (March 2021) |
| spellingShingle | Liposomes (Organelles) Liposomas (Organulos) Inmunogenética Vacuna Herpesviridae Immunogenetics Vaccines Kornuta, Claudia Alejandra Bidart, , Juan Esteban Soria, Ivana Gammella, Mariela Quattrocchi, Valeria Pappalardo, Juan Sebastián Salmaso, Stefano Torchilin, Vladimir P. Cheuquepan Valenzuel, Felipe Andres Hecker, Yanina Moore, Dadin P. Zamorano, Patricia Ines Langellotti, Cecilia Ana MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title | MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title_full | MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title_fullStr | MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title_full_unstemmed | MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title_short | MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1 |
| title_sort | manα1 2man decorated liposomes enhance the immunogenicity induced by a dna vaccine against bohv 1 |
| topic | Liposomes (Organelles) Liposomas (Organulos) Inmunogenética Vacuna Herpesviridae Immunogenetics Vaccines |
| url | http://hdl.handle.net/20.500.12123/8603 https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13718 https://doi.org/10.1111/tbed.13718 |
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