Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum
Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of c...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8601 https://www.sciencedirect.com/science/article/abs/pii/S0022030220303490 https://doi.org/10.3168/jds.2019-17854 |
| _version_ | 1855036065645068288 |
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| author | Lomonaco, Marina Sowul, Mariana Gutierrez, Gerónimo Malacari, Darío Amilcar Alvarez, Irene Porta, Natalia Gabriela Zabal, Osvaldo Alfredo Trono, Karina Gabriela |
| author_browse | Alvarez, Irene Gutierrez, Gerónimo Lomonaco, Marina Malacari, Darío Amilcar Porta, Natalia Gabriela Sowul, Mariana Trono, Karina Gabriela Zabal, Osvaldo Alfredo |
| author_facet | Lomonaco, Marina Sowul, Mariana Gutierrez, Gerónimo Malacari, Darío Amilcar Alvarez, Irene Porta, Natalia Gabriela Zabal, Osvaldo Alfredo Trono, Karina Gabriela |
| author_sort | Lomonaco, Marina |
| collection | INTA Digital |
| description | Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8601 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA86012021-01-15T13:23:55Z Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum Lomonaco, Marina Sowul, Mariana Gutierrez, Gerónimo Malacari, Darío Amilcar Alvarez, Irene Porta, Natalia Gabriela Zabal, Osvaldo Alfredo Trono, Karina Gabriela Bovine Leukaemia Virus Colostrum Spray Drying Virus Leucemia Bovina Calostro Secado por Pulverización Previous studies have shown the presence of bovine leukemia virus (BLV) in colostrum and milk of naturally infected cows. The relationship between virus or provirus and specific antibodies in these secretions is particular to each infected cow and will probably determine whether the consumption of colostrum or milk from these naturally infected dams provides an infective or a protective effect in recipient calves. Our recent findings suggest that this issue is a key point in BLV transmission in very young calves. Based on this, the aim of the present study was to determine the effect of the spray-drying treatment of colostrum on BLV infectivity. The treatment was done on scale-down conditions, using fresh colostrum from BLV-negative cows spiked with infective BLV. Residual infectivity was tested in susceptible lambs. Lambs inoculated with colostrum spiked with BLV-infected cells or cell-free BLV showed evidence of infection 60 d after inoculation, whereas none of the lambs inoculated with spray-dried colostrum showed evidence of infection 60 d after inoculation. These results provide direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious BLV in colostrum. These findings suggest that the risk for BLV transmission could be reduced if milk and colostrum were treated by spray-drying prior to consumption in dairy facilities. The effect of spray-drying on the functional properties and stability of the antibodies present in colostrum under long-term storage should be further investigated. Instituto de Virología Fil: Lomonaco, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Sowul, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Malacari, Darío Amilcar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Porta, Natalia Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Zabal, Osvaldo Alfredo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Sowul, Mariana. Servicio Nacional de Sanidad y Calidad Agroalimentaria (SENASA); Argentina Fil: Alvarez, Irene. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Porta, Natalia Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina 2021-01-15T13:11:01Z 2021-01-15T13:11:01Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8601 https://www.sciencedirect.com/science/article/abs/pii/S0022030220303490 1525-3198 https://doi.org/10.3168/jds.2019-17854 eng info:eu-repograntAgreement/INTA/PNSA-1115054/AR./Enfermedades parasitarias, infecciosas y tóxico metabólicas que afectan la productividad de los bóvidos para producción de carne y leche. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of dairy science 103 (7) : 6504-6510. (Julio 2020) |
| spellingShingle | Bovine Leukaemia Virus Colostrum Spray Drying Virus Leucemia Bovina Calostro Secado por Pulverización Lomonaco, Marina Sowul, Mariana Gutierrez, Gerónimo Malacari, Darío Amilcar Alvarez, Irene Porta, Natalia Gabriela Zabal, Osvaldo Alfredo Trono, Karina Gabriela Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title | Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title_full | Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title_fullStr | Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title_full_unstemmed | Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title_short | Efficacy of the spray-drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| title_sort | efficacy of the spray drying treatment to inactivate the bovine leukemia virus in bovine colostrum |
| topic | Bovine Leukaemia Virus Colostrum Spray Drying Virus Leucemia Bovina Calostro Secado por Pulverización |
| url | http://hdl.handle.net/20.500.12123/8601 https://www.sciencedirect.com/science/article/abs/pii/S0022030220303490 https://doi.org/10.3168/jds.2019-17854 |
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