Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro
Interferon lambda (IFN-λ) is an antiviral naturally produced in response to viral infections, with activity on cells of epithelial origin and located in the mucosal surfaces. This localized activity results in reduced toxicity compared to type I IFNs, whose receptors are ubiquitously expressed. IFN-...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Frontiers Media
2021
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2020.603622/full http://hdl.handle.net/20.500.12123/8588 https://doi.org/10.3389/fvets.2020.603622 |
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| author | Cardoso, Nancy Mansilla, Florencia Celeste Benedetti, Estefanía Turco, Cecilia Soledad Barone, Lucas Jose Iserte, Javier Alonso Soria, Ivana Baumeister, Elsa Capozzo, Alejandra |
| author_browse | Barone, Lucas Jose Baumeister, Elsa Benedetti, Estefanía Capozzo, Alejandra Cardoso, Nancy Iserte, Javier Alonso Mansilla, Florencia Celeste Soria, Ivana Turco, Cecilia Soledad |
| author_facet | Cardoso, Nancy Mansilla, Florencia Celeste Benedetti, Estefanía Turco, Cecilia Soledad Barone, Lucas Jose Iserte, Javier Alonso Soria, Ivana Baumeister, Elsa Capozzo, Alejandra |
| author_sort | Cardoso, Nancy |
| collection | INTA Digital |
| description | Interferon lambda (IFN-λ) is an antiviral naturally produced in response to viral infections, with activity on cells of epithelial origin and located in the mucosal surfaces. This localized activity results in reduced toxicity compared to type I IFNs, whose receptors are ubiquitously expressed. IFN-λ has been effective in the therapy of respiratory viral infections, playing a crucial role in potentiating adaptive immune responses that initiate at mucosal surfaces. Human IFN-λ has polymorphisms that may cause differences in the interaction with the specific receptor in the human population. Interestingly, bovine IFN-λ3 has an in silico-predicted higher affinity for the human receptor than its human counterparts, with high identity with different human IFN-λ variants, making it a suitable antiviral therapeutic candidate for human health. Here, we demonstrate that a recombinant bovine IFN-λ (rbIFN-λ) produced in HEK-293 cells is effective in preventing SARS-CoV-2 infection of VERO cells, with an inhibitory concentration 50% (IC50) between 30 and 50 times lower than that of human type I IFN tested here (α2b and β1a). We also demonstrated the absence of toxicity of rbIFN-λ in human PBMCs and the lack of proinflammatory activity on these cells. Altogether, our results show that rbIFN-λ is as an effective antiviral potentially suitable for COVID-19 therapy. Among other potential applications, rbIFN-λ could be useful to preclude virus dispersion to the lungs and/or to reduce transmission from infected people. Moreover, and due to the non-specific activity of this IFN, it can be potentially effective against other respiratory viruses that may be circulating together with SARS-CoV-2. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8588 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA85882023-07-05T17:40:05Z Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro Cardoso, Nancy Mansilla, Florencia Celeste Benedetti, Estefanía Turco, Cecilia Soledad Barone, Lucas Jose Iserte, Javier Alonso Soria, Ivana Baumeister, Elsa Capozzo, Alejandra COVID-19 Interferonas Viricidas Virus Interferons Antiviral Agents Viruses In vitro Interferon lambda (IFN-λ) is an antiviral naturally produced in response to viral infections, with activity on cells of epithelial origin and located in the mucosal surfaces. This localized activity results in reduced toxicity compared to type I IFNs, whose receptors are ubiquitously expressed. IFN-λ has been effective in the therapy of respiratory viral infections, playing a crucial role in potentiating adaptive immune responses that initiate at mucosal surfaces. Human IFN-λ has polymorphisms that may cause differences in the interaction with the specific receptor in the human population. Interestingly, bovine IFN-λ3 has an in silico-predicted higher affinity for the human receptor than its human counterparts, with high identity with different human IFN-λ variants, making it a suitable antiviral therapeutic candidate for human health. Here, we demonstrate that a recombinant bovine IFN-λ (rbIFN-λ) produced in HEK-293 cells is effective in preventing SARS-CoV-2 infection of VERO cells, with an inhibitory concentration 50% (IC50) between 30 and 50 times lower than that of human type I IFN tested here (α2b and β1a). We also demonstrated the absence of toxicity of rbIFN-λ in human PBMCs and the lack of proinflammatory activity on these cells. Altogether, our results show that rbIFN-λ is as an effective antiviral potentially suitable for COVID-19 therapy. Among other potential applications, rbIFN-λ could be useful to preclude virus dispersion to the lungs and/or to reduce transmission from infected people. Moreover, and due to the non-specific activity of this IFN, it can be potentially effective against other respiratory viruses that may be circulating together with SARS-CoV-2. Instituto de Virología Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Benedetti, Estefanía. Instituto Nacional de Enfermedades Infecciosas, INEI–ANLIS “Dr. Carlos G. Malbrán". Servicio Virosis Respiratorias; Argentina Fil: Turco Cecilia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Iserte, Javier Alonso. Fundación Instituto Leloir. Structural Bioinformatics Group; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Baumeister, Elsa. Instituto Nacional de Enfermedades Infecciosas, INEI–ANLIS “Dr. Carlos G. Malbrán". Servicio Virosis Respiratorias; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-01-08T18:28:54Z 2021-01-08T18:28:54Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.frontiersin.org/articles/10.3389/fvets.2020.603622/full http://hdl.handle.net/20.500.12123/8588 2297-1769 https://doi.org/10.3389/fvets.2020.603622 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Veterinary Science 7 : 603622 (Noviembre 2020) |
| spellingShingle | COVID-19 Interferonas Viricidas Virus Interferons Antiviral Agents Viruses In vitro Cardoso, Nancy Mansilla, Florencia Celeste Benedetti, Estefanía Turco, Cecilia Soledad Barone, Lucas Jose Iserte, Javier Alonso Soria, Ivana Baumeister, Elsa Capozzo, Alejandra Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title | Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title_full | Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title_fullStr | Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title_full_unstemmed | Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title_short | Bovine interferon lambda Is a potent antiviral against SARS-CoV-2 infection in vitro |
| title_sort | bovine interferon lambda is a potent antiviral against sars cov 2 infection in vitro |
| topic | COVID-19 Interferonas Viricidas Virus Interferons Antiviral Agents Viruses In vitro |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2020.603622/full http://hdl.handle.net/20.500.12123/8588 https://doi.org/10.3389/fvets.2020.603622 |
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