Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins
Aims: To study genomic and phenotypic changes in wine yeasts produced in short time periods analysing yeast strains possibly derived from commercial strains recently dispersed. Methods and Results: We conducted a genomic and phenotypic comparison between the commercial yeast strain EC1118 and two n...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8587 https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2010.04689.x https://doi.org/10.1111/j.1365-2672.2010.04689.x |
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| author | Salinas, F. Mandaković, D. Urzua, U. Massera, Ariel Fernando Miras, S. Combina, Mariana Ganga, M. Angélica Martinez, Claudio |
| author_browse | Combina, Mariana Ganga, M. Angélica Mandaković, D. Martinez, Claudio Massera, Ariel Fernando Miras, S. Salinas, F. Urzua, U. |
| author_facet | Salinas, F. Mandaković, D. Urzua, U. Massera, Ariel Fernando Miras, S. Combina, Mariana Ganga, M. Angélica Martinez, Claudio |
| author_sort | Salinas, F. |
| collection | INTA Digital |
| description | Aims: To study genomic and phenotypic changes in wine yeasts produced in short time periods analysing yeast strains possibly derived from commercial strains recently dispersed.
Methods and Results: We conducted a genomic and phenotypic comparison between the commercial yeast strain EC1118 and two novel strains (LV CB and L‐957) isolated from different wine areas industrially intervened <20 years ago. Molecular analysis by amplified fragment length polymorphism (AFLP) and RAPD‐PCR was not able to distinguish between these strains. However, comparative genomic hybridization (aCGH) showed discrete DNA gains and losses that allowed unequivocal identification of the strains. Furthermore, analysis of aCGH data supports the hypothesis that strains LV CB and L‐957 are derivatives from strain EC1118. Finally, scarce phenotypic differences in physiological and metabolic parameters were found among the strains.
Conclusion: The wine yeasts have a very dynamic genome that accumulates changes in short time periods. These changes permit the unique genomic identification of the strains.
Significance and Impact of the Study: This study permits the evaluation of microevolutive events in wine yeasts and its relationship with the phenotype in this species. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8587 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA85872021-01-08T18:19:26Z Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins Salinas, F. Mandaković, D. Urzua, U. Massera, Ariel Fernando Miras, S. Combina, Mariana Ganga, M. Angélica Martinez, Claudio Saccharomyces cerevisiae Levadura de Vino Fenotipos Genotipos Wine Yeast Phenotypes Genotypes Aims: To study genomic and phenotypic changes in wine yeasts produced in short time periods analysing yeast strains possibly derived from commercial strains recently dispersed. Methods and Results: We conducted a genomic and phenotypic comparison between the commercial yeast strain EC1118 and two novel strains (LV CB and L‐957) isolated from different wine areas industrially intervened <20 years ago. Molecular analysis by amplified fragment length polymorphism (AFLP) and RAPD‐PCR was not able to distinguish between these strains. However, comparative genomic hybridization (aCGH) showed discrete DNA gains and losses that allowed unequivocal identification of the strains. Furthermore, analysis of aCGH data supports the hypothesis that strains LV CB and L‐957 are derivatives from strain EC1118. Finally, scarce phenotypic differences in physiological and metabolic parameters were found among the strains. Conclusion: The wine yeasts have a very dynamic genome that accumulates changes in short time periods. These changes permit the unique genomic identification of the strains. Significance and Impact of the Study: This study permits the evaluation of microevolutive events in wine yeasts and its relationship with the phenotype in this species. EEA Mendoza Fil: Salinas, F. Universidad de Santiago de Chile. Facultad Tecnológica. Departamento de Ciencia y Tecnología de los Alimentos; Chile Fil: Mandaković, D. Universidad de Santiago de Chile. Facultad Tecnológica. Departamento de Ciencia y Tecnología de los Alimentos; Chile Fil: Urzua, U. Universidad de Santiago de Chile. Facultad Tecnológica.Laboratorio de Genómica Aplicada, Programa de Biología Celular y Molecular; Chile Fil: Massera, Ariel Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miras, S. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Combina, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ganga, M.A. Universidad de Santiago de Chile. Facultad Tecnológica. Departamento de Ciencia y Tecnología de los Alimentos; Chile Fil: Martínez, C. Universidad de Santiago de Chile. Facultad Tecnológica. Departamento de Ciencia y Tecnología de los Alimentos; Chile 2021-01-08T17:48:30Z 2021-01-08T17:48:30Z 2010-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8587 https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2010.04689.x 1365-2672 https://doi.org/10.1111/j.1365-2672.2010.04689.x eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Journal of Applied Microbiology 108 (5) : 1850-1858 (May 2010) |
| spellingShingle | Saccharomyces cerevisiae Levadura de Vino Fenotipos Genotipos Wine Yeast Phenotypes Genotypes Salinas, F. Mandaković, D. Urzua, U. Massera, Ariel Fernando Miras, S. Combina, Mariana Ganga, M. Angélica Martinez, Claudio Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title | Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title_full | Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title_fullStr | Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title_full_unstemmed | Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title_short | Genomic and phenotypic comparison between similar wine yeast strains of Saccharomyces cerevisiae from different geographic origins |
| title_sort | genomic and phenotypic comparison between similar wine yeast strains of saccharomyces cerevisiae from different geographic origins |
| topic | Saccharomyces cerevisiae Levadura de Vino Fenotipos Genotipos Wine Yeast Phenotypes Genotypes |
| url | http://hdl.handle.net/20.500.12123/8587 https://sfamjournals.onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2672.2010.04689.x https://doi.org/10.1111/j.1365-2672.2010.04689.x |
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