Interferon lambda protects cattle against bovine viral diarrhea virus infection
Interferon lambda (IFN-λ) plays an important role in inducing an antiviral state in mucosal surfaces and has been used as an effective biotherapeutic against several viral diseases. Here we performed a proof of concept study on the activity of a biologically active recombinant bovine IFN-λ (rIFN-λ)...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8582 https://www.sciencedirect.com/science/article/abs/pii/S0165242720301719 https://doi.org/10.1016/j.vetimm.2020.110145 |
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| author | Quintana, Maria Eugenia Cardoso, Nancy Pereyra, Walter Rodrigo Barone, Lucas Jose Barrionuevo, Florencia Mariel Mansilla, Florencia Celeste Turco, Cecilia Soledad Capozzo, Alejandra |
| author_browse | Barone, Lucas Jose Barrionuevo, Florencia Mariel Capozzo, Alejandra Cardoso, Nancy Mansilla, Florencia Celeste Pereyra, Walter Rodrigo Quintana, Maria Eugenia Turco, Cecilia Soledad |
| author_facet | Quintana, Maria Eugenia Cardoso, Nancy Pereyra, Walter Rodrigo Barone, Lucas Jose Barrionuevo, Florencia Mariel Mansilla, Florencia Celeste Turco, Cecilia Soledad Capozzo, Alejandra |
| author_sort | Quintana, Maria Eugenia |
| collection | INTA Digital |
| description | Interferon lambda (IFN-λ) plays an important role in inducing an antiviral state in mucosal surfaces and has been used as an effective biotherapeutic against several viral diseases. Here we performed a proof of concept study on the activity of a biologically active recombinant bovine IFN-λ (rIFN-λ) produced in eukaryotic cells against Bovine Viral Diarrhea Virus (BVDV) in cattle. We first confirmed the lack of toxicity of different concentrations of rIFN-λ in bovine peripheral blood cells and the safety of its subcutaneous application in calves in doses up to 12 IU/kg. The antiviral activity of the rIFN-λ against BVDV was assessed in calves that were inoculated with 6 IU/kg of rIFN-λ (n = 4) or mock-treated (n = 2) two days before and after challenge with a BVDV type-2 non-cytopathic strain. Mock-treated animals developed respiratory disease, shedded the virus from 4 to 7 days post-infection (dpi) and had viremia between 4 and 14 dpi. Conversely, calves treated with rIFN-λ did not develop clinical symptoms. The virus was not found in nasal secretions or sera. Only one animal had a positive viral RNA detection in serum at 7 dpi. All infected animals treated with rIFN-λ increased systemic type-I IFNs levels at 4 dpi. The antiviral treatment induced an earlier onset of the anti-BVDV neutralizing antibodies. Altogether, these results constitute the proof-of-principle of bovine IFN-λ as an antiviral biotherapeutic to protect cattle against the clinical disease caused by BVDV. |
| format | Artículo |
| id | INTA8582 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA85822021-03-09T15:11:19Z Interferon lambda protects cattle against bovine viral diarrhea virus infection Quintana, Maria Eugenia Cardoso, Nancy Pereyra, Walter Rodrigo Barone, Lucas Jose Barrionuevo, Florencia Mariel Mansilla, Florencia Celeste Turco, Cecilia Soledad Capozzo, Alejandra Interferons Bovine Viral Diarrhoea Disease Control Antiviral Agents Cattle Interferonas Diarrea Viral Bovina Control de Enfermedades Viricidas Ganado Bovino Interferon lambda (IFN-λ) plays an important role in inducing an antiviral state in mucosal surfaces and has been used as an effective biotherapeutic against several viral diseases. Here we performed a proof of concept study on the activity of a biologically active recombinant bovine IFN-λ (rIFN-λ) produced in eukaryotic cells against Bovine Viral Diarrhea Virus (BVDV) in cattle. We first confirmed the lack of toxicity of different concentrations of rIFN-λ in bovine peripheral blood cells and the safety of its subcutaneous application in calves in doses up to 12 IU/kg. The antiviral activity of the rIFN-λ against BVDV was assessed in calves that were inoculated with 6 IU/kg of rIFN-λ (n = 4) or mock-treated (n = 2) two days before and after challenge with a BVDV type-2 non-cytopathic strain. Mock-treated animals developed respiratory disease, shedded the virus from 4 to 7 days post-infection (dpi) and had viremia between 4 and 14 dpi. Conversely, calves treated with rIFN-λ did not develop clinical symptoms. The virus was not found in nasal secretions or sera. Only one animal had a positive viral RNA detection in serum at 7 dpi. All infected animals treated with rIFN-λ increased systemic type-I IFNs levels at 4 dpi. The antiviral treatment induced an earlier onset of the anti-BVDV neutralizing antibodies. Altogether, these results constitute the proof-of-principle of bovine IFN-λ as an antiviral biotherapeutic to protect cattle against the clinical disease caused by BVDV. Instituto de Virología Fil: Quintana, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Cardoso, Nancy Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Barone, Lucas Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Barrionuevo, Florencia Mariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Mansilla, Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Turco Cecilia Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-01-08T16:14:54Z 2021-01-08T16:14:54Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8582 https://www.sciencedirect.com/science/article/abs/pii/S0165242720301719 0165-2427 https://doi.org/10.1016/j.vetimm.2020.110145 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Veterinary Immunology and Immunopathology 230 : 110145 (Diciembre 2020) |
| spellingShingle | Interferons Bovine Viral Diarrhoea Disease Control Antiviral Agents Cattle Interferonas Diarrea Viral Bovina Control de Enfermedades Viricidas Ganado Bovino Quintana, Maria Eugenia Cardoso, Nancy Pereyra, Walter Rodrigo Barone, Lucas Jose Barrionuevo, Florencia Mariel Mansilla, Florencia Celeste Turco, Cecilia Soledad Capozzo, Alejandra Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title | Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title_full | Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title_fullStr | Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title_full_unstemmed | Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title_short | Interferon lambda protects cattle against bovine viral diarrhea virus infection |
| title_sort | interferon lambda protects cattle against bovine viral diarrhea virus infection |
| topic | Interferons Bovine Viral Diarrhoea Disease Control Antiviral Agents Cattle Interferonas Diarrea Viral Bovina Control de Enfermedades Viricidas Ganado Bovino |
| url | http://hdl.handle.net/20.500.12123/8582 https://www.sciencedirect.com/science/article/abs/pii/S0165242720301719 https://doi.org/10.1016/j.vetimm.2020.110145 |
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