Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina
Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a comp...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8581 https://www.sciencedirect.com/science/article/pii/S0378111920310143 https://doi.org/10.1016/j.gene.2020.145345 |
| _version_ | 1855484228683169792 |
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| author | Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres |
| author_browse | Cetra, Bibiana Maria Donzelli, María Valeria Maizon, Daniel Omar Medus, Pablo Daniel Periasamy, Kathiravan Pichler, Rudolf Poli, Mario Andres Raschia, Maria Agustina Suarez, Victor Humberto |
| author_facet | Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres |
| author_sort | Raschia, Maria Agustina |
| collection | INTA Digital |
| description | Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed. |
| format | Artículo |
| id | INTA8581 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA85812021-01-08T14:54:09Z Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta Selective breeding of genetically resistant animals is considered a promising strategy to face the problem of nematode resistance to anthelmintics and mitigate concerns about the presence of chemical residues in animal food products and the environment. Gastrointestinal nematode resistance is a complex, multifactorial trait related to host immunity. However, the mechanisms underlying host resistance and response to infection remain to be fully elucidated. In this context, the objective of this study was to provide insight into the chromosomal regions determining nematode resistance and resilience in Corriedale and resistance in Pampinta sheep breeds. A total of 170 single nucleotide polymorphisms (SNP) from 76 candidate genes for immune response were studied in 624 Corriedale and 304 Pampinta animals. Lambs underwent artificial or natural challenges with infective larvae mainly from Haemonchus contortus. Fecal egg counts, estimated breeding values for fecal egg counts, and rate of packed cell volume change and FAMACHA© score change over the challenge were used, when available, as indicators of host parasite resistance or resilience. Phenotype-genotype association studies were conducted and significance values obtained were adjusted for multiple testing errors. Eight SNPs, located on OARs 3, 6, 12, and 20, reached significance in Corriedale sheep under artificial challenge. Those SNP represent allelic variants from the MHC-Ovine Lymphocyte Antigen-DRA, two C-type lectin domain families, the Interleukin 2 receptor β, the Toll-like receptor 10, the Mannan binding lectin serine peptidase 2, and the NLR family, CARD domain containing 4 genes. On Pampinta lambs under natural challenge, we found three significant SNPs, located in the TIMP metallopeptidase inhibitor 3, the FBJ murine osteosarcoma viral oncogene homolog, and the Interleukin 20 receptor alpha genes, on OARs 3, 7, and 8, respectively. The results obtained herein confirm genomic regions previously reported as associated with nematode resistance in other sheep breeds, reinforcing their role in host response to parasites. These findings contribute to gain knowledge on parasite resistance and resilience in Corriedale sheep and report for the first time SNPs associated with resistance to gastrointestinal parasite infections in Pampinta breed. Instituto de Genética Fil: Raschia, Maria Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Donzelli, María Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Medus, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Cetra, Bibiana Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina Fil: Maizon, Daniel Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Suarez, Victor Humberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Pichler, Rudolf. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria Fil: Periasamy, Kathiravan. Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency. Animal Production and Health Laboratory; Austria. Food and Agriculture Organization of the United Nations. Animal Production and Health Division. Animal Genetics Resources Branch; Italia Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina 2021-01-08T14:48:12Z 2021-01-08T14:48:12Z 2021-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8581 https://www.sciencedirect.com/science/article/pii/S0378111920310143 1879-0038 https://doi.org/10.1016/j.gene.2020.145345 eng info:eu-repograntAgreement/INTA/PNBIO-1131033/AR./Genómica y biotecnología aplicada a la cría animal. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Gene 770 : 145345 (Febrero 2021) |
| spellingShingle | Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta Raschia, Maria Agustina Donzelli, María Valeria Medus, Pablo Daniel Cetra, Bibiana Maria Maizon, Daniel Omar Suarez, Victor Humberto Pichler, Rudolf Periasamy, Kathiravan Poli, Mario Andres Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title | Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title_full | Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title_fullStr | Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title_full_unstemmed | Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title_short | Single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in Corriedale and Pampinta sheep in Argentina |
| title_sort | single nucleotide polymorphisms from candidate genes associated with nematode resistance and resilience in corriedale and pampinta sheep in argentina |
| topic | Genes Ovinos Mecanismos de Defensa Polimorfismo de un Solo Nucleótido Resiliencia Argentina Sheep Defence Mechanisms Single Nucleotide Polymorphism Resilience Candidate Genes Genes Candidatos Raza Corriedale Raza Pampinta |
| url | http://hdl.handle.net/20.500.12123/8581 https://www.sciencedirect.com/science/article/pii/S0378111920310143 https://doi.org/10.1016/j.gene.2020.145345 |
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