Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from P...

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Autores principales: Carneiro, Paulo A.M., Pasquatti, Taynara N., Takatani, Haruo, Zumarraga, Martin Jose, Marfil, Maria Jimena, Barnard, Christian, Fitzgerald, Scott D., Abramovitch, Robert B., Araujo, Flabio Ribeiro de, Kaneene, John B.
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Wiley 2021
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/8553
https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203
https://doi.org/10.1002/vms3.203
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author Carneiro, Paulo A.M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
author_browse Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Barnard, Christian
Carneiro, Paulo A.M.
Fitzgerald, Scott D.
Kaneene, John B.
Marfil, Maria Jimena
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
author_facet Carneiro, Paulo A.M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
author_sort Carneiro, Paulo A.M.
collection INTA Digital
description The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
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spelling INTA85532021-01-05T18:06:05Z Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil Carneiro, Paulo A.M. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Marfil, Maria Jimena Barnard, Christian Fitzgerald, Scott D. Abramovitch, Robert B. Araujo, Flabio Ribeiro de Kaneene, John B. Mycobacterium bovis Tuberculosis Ganado Bovino Razas de Búfalo Genotipos Brasil Cattle Buffalo Breeds Genotypes The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil. Instituto de Biotecnología Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil Fil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidos Fil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos Fil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasil Fil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos 2021-01-05T17:58:19Z 2021-01-05T17:58:19Z 2020-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8553 https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203 2053-1095 https://doi.org/10.1002/vms3.203 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)
spellingShingle Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
Carneiro, Paulo A.M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_fullStr Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full_unstemmed Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_short Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_sort molecular characterization of mycobacterium bovis infection in cattle and buffalo in amazon region brazil
topic Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
url http://hdl.handle.net/20.500.12123/8553
https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203
https://doi.org/10.1002/vms3.203
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