Development of a duplex PCR for the identification of Fasciola hepatica in lymnaeid snails
Fasciola hepatica is a parasitic trematode that causes fascioliasis, a disease that affects domestic livestock and humans. The complex life cycle of F. hepatica involves lymnaeid snails as intermediate hosts. Detection of F. hepatica in snails is a useful tool for the control of fascioliasis in live...
| Main Authors: | , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Ediciones INTA
2020
|
| Subjects: | |
| Online Access: | http://ria.inta.gob.ar/contenido/ria-46-no-3-diciembre-2020 http://hdl.handle.net/20.500.12123/8522 |
| Summary: | Fasciola hepatica is a parasitic trematode that causes fascioliasis, a disease that affects domestic livestock and humans. The complex life cycle of F. hepatica involves lymnaeid snails as intermediate hosts. Detection of F. hepatica in snails is a useful tool for the control of fascioliasis in livestock. Detection methods involve crushing the snails and microscopic observation, but have low sensitivity and are time-consuming. To overcome these disadvantages, researchers are developing molecular methods. In this work, we developed a duplex PCR that allows the detection of F. hepatica in snails as two single and bright bands: one corresponding to the parasite and one to the snail, the latter of which works as an internal control to detect PCR inhibitors. To avoid false-positive results, we also evaluated the method of disinfection of the material used for snail collection. The duplex PCR developed showed a sensitivity high enough to detect a single miracidium per snail, and significantly shortened the time required to analyze a large number of snails. |
|---|