Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study
Bovine viral diarrhea (BVD) is a major worldwide disease with negative economic impact on cattle production. Successful control programs of BVD require the identification and culling of persistently infected (PI) animals with bovine viral diarrhea virus (BVDV). A variety of diagnostic tests are avai...
| Autores principales: | , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8463 https://link.springer.com/article/10.1007/s42770-020-00410-1 https://doi.org/10.1007/s42770-020-00410-1 |
| _version_ | 1855036039968587776 |
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| author | Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Alvarez, Ignacio Norero, Natalia Sigrid Storani, Leonardo Pereyra, Susana Beatriz Verna, Andrea Elizabeth Odeon, Anselmo Carlos Gonzalez Altamiranda, Erika |
| author_browse | Alvarez, Ignacio Armendano, Joaquín Ignacio Gonzalez Altamiranda, Erika Louge Uriarte, Enrique Leopoldo Norero, Natalia Sigrid Odeon, Anselmo Carlos Pereyra, Susana Beatriz Spetter, Lucas Maximiliano Storani, Leonardo Verna, Andrea Elizabeth |
| author_facet | Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Alvarez, Ignacio Norero, Natalia Sigrid Storani, Leonardo Pereyra, Susana Beatriz Verna, Andrea Elizabeth Odeon, Anselmo Carlos Gonzalez Altamiranda, Erika |
| author_sort | Spetter, Lucas Maximiliano |
| collection | INTA Digital |
| description | Bovine viral diarrhea (BVD) is a major worldwide disease with negative economic impact on cattle production. Successful control programs of BVD require the identification and culling of persistently infected (PI) animals with bovine viral diarrhea virus (BVDV). A variety of diagnostic tests are available to detect BVDV, but no comparison has been performed among those tests in Argentina. Sera collected from 2864 cattle, belonging to 55 herds from three Argentinean provinces, were analyzed by nested RT-PCR (RT-nPCR) to detect BVDV for diagnostic purposes. Additionally, this study evaluated the agreement of the RT-nPCR along with virus isolation, antigen-capture ELISA, and real-time RT-PCR for BVDV detection in archived bovine serum samples (n = 90). The RT-nPCR was useful for BVDV detection in pooled and individual serum samples. BVDV was detected in 1% (29/2864) of the cattle and in 20% (11/55) of the herds. The proportion of BVDV-positive sera was not statistically different among the tests. In addition, comparisons showed high agreement levels, with the highest values between both RT-PCR protocols. The frequency of BVDV infection at individual and herd level was lower than the reported values worldwide. Since follow-up testing was not performed, the frequency of PI cattle was unknown. Also, this study demonstrated that the four diagnostic tests can be used reliably for BVDV identification in individual serum samples. Further epidemiologically designed studies that address prevalence, risk factors, and economic impact of BVDV in Argentina will be necessary to implement effective control programs. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8463 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA84632020-12-21T11:20:35Z Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Alvarez, Ignacio Norero, Natalia Sigrid Storani, Leonardo Pereyra, Susana Beatriz Verna, Andrea Elizabeth Odeon, Anselmo Carlos Gonzalez Altamiranda, Erika Ganado Bovino Diarrea Viral Bovina Diagnóstico Pestivirus Enfermedades de los Animales Argentina Cattle Bovine Viral Diarrhoea Diagnosis Animals Diseases Bovine viral diarrhea (BVD) is a major worldwide disease with negative economic impact on cattle production. Successful control programs of BVD require the identification and culling of persistently infected (PI) animals with bovine viral diarrhea virus (BVDV). A variety of diagnostic tests are available to detect BVDV, but no comparison has been performed among those tests in Argentina. Sera collected from 2864 cattle, belonging to 55 herds from three Argentinean provinces, were analyzed by nested RT-PCR (RT-nPCR) to detect BVDV for diagnostic purposes. Additionally, this study evaluated the agreement of the RT-nPCR along with virus isolation, antigen-capture ELISA, and real-time RT-PCR for BVDV detection in archived bovine serum samples (n = 90). The RT-nPCR was useful for BVDV detection in pooled and individual serum samples. BVDV was detected in 1% (29/2864) of the cattle and in 20% (11/55) of the herds. The proportion of BVDV-positive sera was not statistically different among the tests. In addition, comparisons showed high agreement levels, with the highest values between both RT-PCR protocols. The frequency of BVDV infection at individual and herd level was lower than the reported values worldwide. Since follow-up testing was not performed, the frequency of PI cattle was unknown. Also, this study demonstrated that the four diagnostic tests can be used reliably for BVDV identification in individual serum samples. Further epidemiologically designed studies that address prevalence, risk factors, and economic impact of BVDV in Argentina will be necessary to implement effective control programs. EEA Balcarce Fil: Spetter, Lucas Maximiliano. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Louge Uriarte Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: Armendano Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina Fil: Álvarez Ignacio, Laboratorio Álvarez; Argentina Fil: Norero Natalia Sigrid. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: Storani, Leonardo. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pereyra, Susana Beatriz, Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: Odeón, Anselmo Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina Fil: González Altamiranda EA Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible Balcarce; Argentina 2020-12-21T11:11:20Z 2020-12-21T11:11:20Z 2020-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8463 https://link.springer.com/article/10.1007/s42770-020-00410-1 1678-4405 (online) 1517-8382 (print) https://doi.org/10.1007/s42770-020-00410-1 eng info:eu-repograntAgreement/INTA/PNSA-1115053/AR./Biotecnologías reproductivas y desarrollo de metodologías de diagnóstico, control y prevención de las enfermedades infecciosas y parasitarias que afectan la concepción, gestación y período neonatal en especies de interés zootécnico. info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Brazilian Journal of Microbiology (2020) |
| spellingShingle | Ganado Bovino Diarrea Viral Bovina Diagnóstico Pestivirus Enfermedades de los Animales Argentina Cattle Bovine Viral Diarrhoea Diagnosis Animals Diseases Spetter, Lucas Maximiliano Louge Uriarte, Enrique Leopoldo Armendano, Joaquín Ignacio Alvarez, Ignacio Norero, Natalia Sigrid Storani, Leonardo Pereyra, Susana Beatriz Verna, Andrea Elizabeth Odeon, Anselmo Carlos Gonzalez Altamiranda, Erika Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title | Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title_full | Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title_fullStr | Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title_full_unstemmed | Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title_short | Frequency of bovine viral diarrhea virus (BVDV) in Argentinean bovine herds and comparison of diagnostic tests for BVDV detection in bovine serum samples: a preliminary study |
| title_sort | frequency of bovine viral diarrhea virus bvdv in argentinean bovine herds and comparison of diagnostic tests for bvdv detection in bovine serum samples a preliminary study |
| topic | Ganado Bovino Diarrea Viral Bovina Diagnóstico Pestivirus Enfermedades de los Animales Argentina Cattle Bovine Viral Diarrhoea Diagnosis Animals Diseases |
| url | http://hdl.handle.net/20.500.12123/8463 https://link.springer.com/article/10.1007/s42770-020-00410-1 https://doi.org/10.1007/s42770-020-00410-1 |
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