Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome
Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8439 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12964 https://doi.org/10.1111/ppa.12964 |
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| author | Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Declerck, Stéphane Ducasse, Daniel Adrian |
| author_browse | Debat, Humberto Julio Declerck, Stéphane Ducasse, Daniel Adrian Gallou, Adrien Giachero, María Lorena Marquez, Nathalie |
| author_facet | Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Declerck, Stéphane Ducasse, Daniel Adrian |
| author_sort | Marquez, Nathalie |
| collection | INTA Digital |
| description | Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture
experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of
up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme. |
| format | Artículo |
| id | INTA8439 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | INTA84392020-12-16T16:25:42Z Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Declerck, Stéphane Ducasse, Daniel Adrian Mycorrhizae Fusarium Soja Soybeans Fusarium Virguliforme Mycorrhiza Sudden Death Syndrome Transcriptome Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome (SDS) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS. Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme, was observed in the presence of arbuscular mycorrhizal fungi (AMF), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme, grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF-colonized (+AMF+Fv) and noncolonized ( AMF+Fv) plants, respectively. Major transcriptional changes corresponded to defence response-related genes belonging to secondary metabolism, stress and signalling categories. The +AMF+Fv treatment showed the largest number of up-regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the +AMF+Fv treatment, such as those encoding a thaumatin-like protein (TLP) and a pleiotropic drug resistance (PDR) protein. Moreover, +AMF+Fv showed a significantly greater number of down-regulated genes related to cell wall modification and peroxidases than the –AMF+Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme. Instituto de Patología Vegetal Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México. Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology. Mycology. Louvain-la-Neuve; Bélgica Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina 2020-12-16T16:16:22Z 2020-12-16T16:16:22Z 2018-11-21 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8439 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12964 0032-0862 1365-3059 (online) https://doi.org/10.1111/ppa.12964 eng info:eu-repo/semantics/restrictedAccess application/pdf Plant Pathology 68 (3) : 470- 480 (2019) |
| spellingShingle | Mycorrhizae Fusarium Soja Soybeans Fusarium Virguliforme Mycorrhiza Sudden Death Syndrome Transcriptome Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Declerck, Stéphane Ducasse, Daniel Adrian Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title | Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title_full | Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title_fullStr | Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title_full_unstemmed | Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title_short | Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme, one causal agent of sudden death syndrome |
| title_sort | transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with fusarium virguliforme one causal agent of sudden death syndrome |
| topic | Mycorrhizae Fusarium Soja Soybeans Fusarium Virguliforme Mycorrhiza Sudden Death Syndrome Transcriptome |
| url | http://hdl.handle.net/20.500.12123/8439 https://bsppjournals.onlinelibrary.wiley.com/doi/abs/10.1111/ppa.12964 https://doi.org/10.1111/ppa.12964 |
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