Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina
Macrophomina phaseolina is a soil-borne fungal pathogen with a wide host range that causes charcoal rot in soybean [Glycine max (L.) Merr.]. Control of the disease is a challenge, due to the absence of genetic resistance and effective chemical control. Alternative or complementary measures are neede...
| Main Authors: | , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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American Phytopathological Society
2020
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| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-17-0282-R http://hdl.handle.net/20.500.12123/8435 https://doi.org/10.1094/MPMI-11-17-0282-R |
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| author | Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Cranenbrouck, Sylvie Di Rienzo, Julio A. Pozo, María J. Ducasse, Daniel Adrian Declerck, Stéphane |
| author_browse | Cranenbrouck, Sylvie Debat, Humberto Julio Declerck, Stéphane Di Rienzo, Julio A. Ducasse, Daniel Adrian Gallou, Adrien Giachero, María Lorena Marquez, Nathalie Pozo, María J. |
| author_facet | Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Cranenbrouck, Sylvie Di Rienzo, Julio A. Pozo, María J. Ducasse, Daniel Adrian Declerck, Stéphane |
| author_sort | Marquez, Nathalie |
| collection | INTA Digital |
| description | Macrophomina phaseolina is a soil-borne fungal pathogen with a wide host range that causes charcoal rot in soybean [Glycine max (L.) Merr.]. Control of the disease is a challenge, due to the absence of genetic resistance and effective chemical control. Alternative or complementary measures are needed, such as the use of biological control agents, in an integrated approach. Several studies have demonstrated the role of arbuscular mycorrhizal fungi (AMF) in enhancing plant resistance or tolerance to biotic stresses, decreasing the symptoms and pressure caused by various pests and diseases, including M. phaseolina in soybean. However, the specific contribution of AMF in the regulation of the plant response to M. phaseolina remains unclear. Therefore, the objective of the present study was to investigate, under strict in-vitro culture conditions, the global transcriptional changes in roots of premycorrhized soybean plantlets challenged by M. phaseolina (+AMF+Mp) as compared with nonmycorrhizal soybean plantlets (-AMF+Mp). MapMan software was used to distinguish transcriptional changes, with special emphasis on those related to plant defense responses. Soybean genes identified as strongly upregulated during infection by the pathogen included pathogenesis-related proteins, disease-resistance proteins, transcription factors, and secondary metabolism-related genes, as well as those encoding for signaling hormones. Remarkably, the +AMF+Mp treatment displayed a lower number of upregulated genes as compared with the -AMF+Mp treatment. AMF seemed to counteract or balance costs upon M. phaseolina infection, which could be associated to a negative impact on biomass and seed production. These detailed insights in soybean-AMF interaction help us to understand the complex underlying mechanisms involved in AMF-mediated biocontrol and support the importance of preserving and stimulating the existing plant-AMF associates, via adequate agricultural practices, to optimize their agro-ecological potential. |
| format | Artículo |
| id | INTA8435 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA84352020-12-16T11:32:50Z Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Cranenbrouck, Sylvie Di Rienzo, Julio A. Pozo, María J. Ducasse, Daniel Adrian Declerck, Stéphane Soybeans Plants Mycorrhizal Infection Stress Macrophomina Phaseolina Soja Plantas Infección de Micorrizas Estres Mycorrhizal Macrophomina phaseolina is a soil-borne fungal pathogen with a wide host range that causes charcoal rot in soybean [Glycine max (L.) Merr.]. Control of the disease is a challenge, due to the absence of genetic resistance and effective chemical control. Alternative or complementary measures are needed, such as the use of biological control agents, in an integrated approach. Several studies have demonstrated the role of arbuscular mycorrhizal fungi (AMF) in enhancing plant resistance or tolerance to biotic stresses, decreasing the symptoms and pressure caused by various pests and diseases, including M. phaseolina in soybean. However, the specific contribution of AMF in the regulation of the plant response to M. phaseolina remains unclear. Therefore, the objective of the present study was to investigate, under strict in-vitro culture conditions, the global transcriptional changes in roots of premycorrhized soybean plantlets challenged by M. phaseolina (+AMF+Mp) as compared with nonmycorrhizal soybean plantlets (-AMF+Mp). MapMan software was used to distinguish transcriptional changes, with special emphasis on those related to plant defense responses. Soybean genes identified as strongly upregulated during infection by the pathogen included pathogenesis-related proteins, disease-resistance proteins, transcription factors, and secondary metabolism-related genes, as well as those encoding for signaling hormones. Remarkably, the +AMF+Mp treatment displayed a lower number of upregulated genes as compared with the -AMF+Mp treatment. AMF seemed to counteract or balance costs upon M. phaseolina infection, which could be associated to a negative impact on biomass and seed production. These detailed insights in soybean-AMF interaction help us to understand the complex underlying mechanisms involved in AMF-mediated biocontrol and support the importance of preserving and stimulating the existing plant-AMF associates, via adequate agricultural practices, to optimize their agro-ecological potential. Instituto de Patología Vegetal Fil: Marquez, Nathalie. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Giachero, María Lorena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Gallou, Adrien. Centro Nacional de Referencia de Control Biológico; México. Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Cranenbrouck, Sylvie. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology, Mycology. Mycothèque de l'Université Catholique de Louvain (MUCL). Belgian Coordinated Collections of Microorganisms (BCCM); Bélgica Fil: Di Rienzo, Julio A. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Estadística y Biometría; Argentina Fil: Pozo, María J. Estación Experimental del Zaidín. Department of Soil Microbiology and Symbiotic Systems; España Fil: Ducasse, Daniel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Declerck, Stéphane. Université Catholique de Louvain. Earth and Life Institute. Applied Microbiology. Mycology. Louvain-la-Neuve; Bélgica 2020-12-16T11:22:27Z 2020-12-16T11:22:27Z 2018-06-19 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-17-0282-R http://hdl.handle.net/20.500.12123/8435 0894-0282 https://doi.org/10.1094/MPMI-11-17-0282-R eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Molecular Plant-Microbe Interactions 31 (8) : 842-855 (2018) |
| spellingShingle | Soybeans Plants Mycorrhizal Infection Stress Macrophomina Phaseolina Soja Plantas Infección de Micorrizas Estres Mycorrhizal Marquez, Nathalie Giachero, María Lorena Gallou, Adrien Debat, Humberto Julio Cranenbrouck, Sylvie Di Rienzo, Julio A. Pozo, María J. Ducasse, Daniel Adrian Declerck, Stéphane Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title | Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title_full | Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title_fullStr | Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title_full_unstemmed | Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title_short | Transcriptional Changes in Mycorrhizal and Nonmycorrhizal Soybean Plants upon Infection with the Fungal Pathogen Macrophomina phaseolina |
| title_sort | transcriptional changes in mycorrhizal and nonmycorrhizal soybean plants upon infection with the fungal pathogen macrophomina phaseolina |
| topic | Soybeans Plants Mycorrhizal Infection Stress Macrophomina Phaseolina Soja Plantas Infección de Micorrizas Estres Mycorrhizal |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-11-17-0282-R http://hdl.handle.net/20.500.12123/8435 https://doi.org/10.1094/MPMI-11-17-0282-R |
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