Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure
The effects of 17α-ethinylestradiol (EE2) on sex ratio, gonopodium morphology, and gonadal histology of C. decemmaculatus were assessed by a full-lifecycle exposure experiment. Newborn fish were waterborne exposed to 30, 100, and 300 ng EE2/L for 90 d, using 50 fish per treatment. Additionally, in D...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Elsevier
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/8401 https://www.sciencedirect.com/science/article/pii/S0147651320310150 https://doi.org/10.1016/j.ecoenv.2020.111176 |
| _version_ | 1855484191565676544 |
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| author | Young, Brian Jonathan Cristos, Diego Sebastian Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro |
| author_browse | Carriquiriborde, Pedro Crespo, Diana Cristina Cristos, Diego Sebastian Somoza, Gustavo Manuel Young, Brian Jonathan |
| author_facet | Young, Brian Jonathan Cristos, Diego Sebastian Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro |
| author_sort | Young, Brian Jonathan |
| collection | INTA Digital |
| description | The effects of 17α-ethinylestradiol (EE2) on sex ratio, gonopodium morphology, and gonadal histology of C. decemmaculatus were assessed by a full-lifecycle exposure experiment. Newborn fish were waterborne exposed to 30, 100, and 300 ng EE2/L for 90 d, using 50 fish per treatment. Additionally, in December of 2016, a field survey was conducted on a C. decemmaculatus population inhabiting the Girado Creek downstream of the Chascomus city wastewater effluent discharge. After 90 d of exposure, EE2 was able to histologically skew the sex ratio toward females and inhibit the full gonopodium development since the lowest tested concentration (LOEC = 30 ng/L). At higher concentrations, EE2 was toxic, inducing mortality in a concentration-dependent fashion (90 d-LC50 = 109.9 ng/L) and altering the gonadal histoarchitecture, causing neither testes nor ovaries discernible histologically (LOEC = 100 ng/L). In addition, a novel response, perianal hyperpigmentation, was discovered been induced by the EE2 exposure in a concentration-dependent fashion (90 d-EC50 = 39.3 ng/L). A higher proportion of females and perianal hyperpigmentation were observed in wild fish collected from the Girado Creek. The major reached conclusions are: i) EE2 induce different effects on the sexual traits of C. decemmaculatus when exposed from early-life or adult stages. ii) The most sensitive effects observed in the laboratory occur in a creek receiving wastewater effluent. iii) The perianal hyperpigmentation comes-up as a promising biomarker of exposure to estrogenic compounds. |
| format | Artículo |
| id | INTA8401 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA84012020-12-11T20:05:09Z Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure Young, Brian Jonathan Cristos, Diego Sebastian Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro Melanins Genetic Markers Melaninas Marcadores Genéticos Endocrine Disruption Xenoestrogen Secondary Sexual Characteristics Biomarker Alteración Endocrina Xenoestrógeno Características Sexuales Secundarias Biomarcador Teleostei The effects of 17α-ethinylestradiol (EE2) on sex ratio, gonopodium morphology, and gonadal histology of C. decemmaculatus were assessed by a full-lifecycle exposure experiment. Newborn fish were waterborne exposed to 30, 100, and 300 ng EE2/L for 90 d, using 50 fish per treatment. Additionally, in December of 2016, a field survey was conducted on a C. decemmaculatus population inhabiting the Girado Creek downstream of the Chascomus city wastewater effluent discharge. After 90 d of exposure, EE2 was able to histologically skew the sex ratio toward females and inhibit the full gonopodium development since the lowest tested concentration (LOEC = 30 ng/L). At higher concentrations, EE2 was toxic, inducing mortality in a concentration-dependent fashion (90 d-LC50 = 109.9 ng/L) and altering the gonadal histoarchitecture, causing neither testes nor ovaries discernible histologically (LOEC = 100 ng/L). In addition, a novel response, perianal hyperpigmentation, was discovered been induced by the EE2 exposure in a concentration-dependent fashion (90 d-EC50 = 39.3 ng/L). A higher proportion of females and perianal hyperpigmentation were observed in wild fish collected from the Girado Creek. The major reached conclusions are: i) EE2 induce different effects on the sexual traits of C. decemmaculatus when exposed from early-life or adult stages. ii) The most sensitive effects observed in the laboratory occur in a creek receiving wastewater effluent. iii) The perianal hyperpigmentation comes-up as a promising biomarker of exposure to estrogenic compounds. IMyZA Fil: Young, Brian Jonathan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Fil: Cristos, Diego Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Tecnología de Alimentos; Argentina. Fil: Crespo, Diana Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Fil: Somoza, Gustavo Manuel. Instituto Tecnológico de Chascomús (CONICET-UNSAM); Argentina. Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones Del Medioambiente. Facultad de Ciencias Exactas; Argentina. 2020-12-11T19:45:31Z 2020-12-11T19:45:31Z 2020-08-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8401 https://www.sciencedirect.com/science/article/pii/S0147651320310150 0147-6513 https://doi.org/10.1016/j.ecoenv.2020.111176 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Ecotoxicology and Environmental Safety 205 : art. 111176. (2020). |
| spellingShingle | Melanins Genetic Markers Melaninas Marcadores Genéticos Endocrine Disruption Xenoestrogen Secondary Sexual Characteristics Biomarker Alteración Endocrina Xenoestrógeno Características Sexuales Secundarias Biomarcador Teleostei Young, Brian Jonathan Cristos, Diego Sebastian Crespo, Diana Cristina Somoza, Gustavo Manuel Carriquiriborde, Pedro Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title | Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title_full | Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title_fullStr | Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title_full_unstemmed | Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title_short | Effects of 17α-ethinylestradiol on sex ratio, gonadal histology and perianal hyperpigmentation of Cnesterodon decemmaculatus (Pisces, Poeciliidae) during a full-lifecycle exposure |
| title_sort | effects of 17α ethinylestradiol on sex ratio gonadal histology and perianal hyperpigmentation of cnesterodon decemmaculatus pisces poeciliidae during a full lifecycle exposure |
| topic | Melanins Genetic Markers Melaninas Marcadores Genéticos Endocrine Disruption Xenoestrogen Secondary Sexual Characteristics Biomarker Alteración Endocrina Xenoestrógeno Características Sexuales Secundarias Biomarcador Teleostei |
| url | http://hdl.handle.net/20.500.12123/8401 https://www.sciencedirect.com/science/article/pii/S0147651320310150 https://doi.org/10.1016/j.ecoenv.2020.111176 |
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